ABSTRACT
RESUMEN El desarrollo masivo de cianobacterias y la contaminación por cianotoxinas constituyen una problemática ambiental de especial interés en los sistemas acuáticos, debido a los diversos impactos negativos que los florecimientos (blooms) de cianobacterias pueden ocasionar. Dada la importancia recreacional y de abastecimiento de los embalses Abreo - Malpaso, Peñol - Guatapé y Playas, ubicados en el oriente de Antioquia - Colombia, en este estudio, se estimó la densidad de cianobacterias planctónicas y otros organismos del fitoplancton, así como el biovolumen geométrico, la relación superficie volumen (S/V) y la dimensión lineal máxima (DLM), a través de cuatro muestreos, en tres estaciones, para cada uno de los embalses. Se identificaron, dentro del phylum Cyanobacteria, con potencial de producción de cianotoxinas, el complejo Microcystis, los géneros Woronichinia, Aphanocapsa y Oscillatoria y la especie Radiocystis fernandoi. Al aplicar la prueba no parámetrica Kruskal - Wallis, no se encontraron diferencias significativas entre los embalses estudiados (p>0,05); sin embargo, el embalse El Peñol - Guatapé presentó la mayor biomasa algal y densidad de cianobacterias, por lo que, según los niveles de riesgo planteados para los embalses estudiados y de acuerdo con las características morfológicas de estos organismos, este embalse presenta un nivel de riesgo Medio Alto con relación a la presencia y morfología de las cianobacterias, mientras que los embalses Abreo - Malpaso y Playas están asociados a un riesgo Medio Bajo. Se sugiere establecer medidas de control y de prevención, con el fin de evitar un desarrollo masivo de cianobacterias, una potencial presencia de cianotoxinas y afectaciones graves a seres humanos y animales.
ABSTRACT Cyanotoxin contamination is of special interest in water bodies, especially those for human use, due to the various negative effects that blooms of potentially toxic cyanobacteria can cause on human health. Given the recreational and supply importance of the Abreo - Malpaso, Peñol - Guatapé and Playas reservoirs in the Department of Antioquia, Colombia. This study has estimated the biomass of the planktonic cyanobacteria, their geometric biovolume, the surface volume ratio (S / V) and the maximum linear dimension (MLD), during four sampling seasons in three different stations for each of these bodies. Water. We have identified from the Cyanophyta division with the production of cyanotoxins, the Microcystis complex and generates Woronichinia, Aphanocapsa and Oscillatoria, as well as the species Radiocystis fernandoi. When applying the nonparametric Kruskal - Wallis test, no significant differences were found between the studied reservoirs (p> 0.05); however, the Peñol - Guatapé reservoir presented the highest algae biomass and density of cyanobacteria, therefore, according to the levels of risk posed for the reservoirs studied and according to the morphological characteristics of these organisms, this reservoir presents a High Medium risk level in relation to the presence and morphology of the cyanobacteria, while the Abreo-Malpaso and Playas reservoirs are associated with a Medium Low risk. It is suggested to establish control and prevention measures to avoid a massive development of cyanobacteria, a possible presence of cyanotoxins and serious affectations in humans and animals.
ABSTRACT
ResumenLa morfología funcional corresponde a las respuestas de adaptación a los cambios en el medio ambiente. En fitoplancton, rasgos como el desarrollo de mucílago, vesículas de aceite, filamentos y variación en la relación superficie/ volumen, permiten enfrentar la sedimentación, optimizar la captación lumínica y de nutrientes. El objetivo del presente estudio fue evaluar la relación entre los rasgos y la variación de las condiciones físicas y químicas en el lago de Tota (Boyacá, Colombia). Para esto, se realizaron diez muestreos mensuales entre octubre 2013 y julio 2014. Se midieron el pH, conductividad eléctrica, temperatura, oxígeno disuelto, saturación de oxígeno, alcalinidad y dureza total del agua. Además se recolectaron muestras para la cuantificación en laboratorio de Nitrógeno Total Kjeldahl y Fósforo Total. Las algas se obtuvieron en diferentes profundidades según la transparencia Secchi. Se caracterizó la composición taxonómica y se estimó la abundancia por el método de sedimentación en cámaras. Se midieron los rasgos morfológicos de cada taxa según la correspondencia a modelos geométricos, se clasificaron en grupos funcionales basados en morfología (GFBM) y se calculó su biomasa expresada como biovolumen. Las variables fueron evaluadas mediante Análisis de Componentes Principales, que incluyó el efecto espacio-temporal y fue interpretado según la variación en el nivel del agua. Mediante Análisis de Correspondencia Canónica se estableció la relación entre el biovolumen del fitoplancton acumulado en categorías de forma y las variables ambientales. La ordenación mostró que las condiciones limnológicas son influenciadas por cambios estacionales, principalmente representadas por el porcentaje de saturación de oxígeno, la transparencia Secchi, el NTK, la dureza y la alcalinidad. Se encontraron cuatro GFBM: IV, V, VI y VII, siendo los GFBM IV y VII, los más representativos. El ACC fue significativo (Test de Monte-Carlo, p<0.05). En conclusión, la respuesta morfológica y funcional del fitoplancton del lago de Tota esta condicionada principalmente por la variabilidad a corto plazo, en la transparencia, el fósforo, el nitrógeno, la dureza y la conductividad eléctrica, que son afectados por los cambios estacionales en el nivel del lago.
AbstractFunctional morphology corresponds to adaptive responses to changes in the environment. In phytoplankton, traits such as the development of mucilage, oil vesicles, filaments and variation in the surface/volume ratio, allow algae to deal with sedimentation, optimizing light and nutrient uptake. The aim of this research was to evaluate the relationship between traits and variation in physical and chemical conditions in Tota Lake (Boyacá, Colombia). For this, ten samplings were undertaken in a monthly basis between October 2013 and July 2014. We measured water pH, conductivity, temperature, dissolved oxygen, oxygen saturation, alkalinity and total hardness; we also quantified Total Kjeldahl nitrogen and Total phosphorus. Algae were collected at different depths according to Secchi transparency. Taxonomic composition and abundance were estimated by the chamber sedimentation method. Morphological traits were measured for each taxa according to geometric models, allowing traits classification into morphologically based functional groups (MBFG) and having algae biomass expressed as biovolume. The variables were evaluated through Principal Components Analysis, which included time-space effect and it was interpreted according to variation in water level. Through Canonical Correspondence Analysis we established the relationship between phytoplankton biovolume accumulated in form categories and environmental variables. The ordination showed that limnological conditions are influenced by seasonal changes, which are mainly represented by oxygen saturation percentage, Secchi transparency, TKN, hardness and alkalinity. Four MBFG were found: IV, V, VI and VII, being MBFG IV and VII the most representatives. ACC was significant (Monte-Carlo Test, p<0.05). In conclusion, the morphological and functional response of phytoplankton in Tota Lake is driven by short-term variability in transparency, phosphorus, nitrogen, hardness, and electrical conductivity, which are all affected by seasonal changes in the lake level.
ABSTRACT
Abstract This study aimed to evaluate nutrients concentration and spatial-temporal changes in phytoplankton biovolume during an experimental fish culture in net cages in a lateral arm of Salto Caxias reservoir, Brazil. Two sampling stations were placed in the affected lateral arm and other two in a cageless lateral arm. Neither abiotic variables nor phytoplankton biovolume presented significant differences between the treatments. Only temporal changes were confirmed by the analysis performed. Both lateral arms were classified as oligotrophic, reflecting low influence of the net cages. Phytoplankton growth seems to be limited by nitrogen. Biovolume values were, in general, low and five major functional groups were recognized (E, F, G, K and P). In summer higher biovolume values were observed and representatives of Chlorophyceae and Cyanobacteria belonging to the functional groups F and K, respectively, were the most important. In winter phytoplankton was mainly composed by Bacillariophyceae taxa from P group. G group was also restricted to winter and E group occurred in winter and summer. The variations recorded in phytoplankton structure appear to have been mainly influenced by seasonal changes in temperature, precipitation and nutrients availability. The effects of net cages on the abiotic variables and phytoplankton biovolume appear to have been small, probably due to the small number of net cages employed and the system dilution capacity. However, a permanent monitoring of phytoplankton is recommended, since this environment has a carrying capacity, from which the trophic state may increase.
Resumo Este estudo objetivou avaliar alterações nas variáveis abióticas e no biovolume fitoplanctônico durante o cultivo experimental de peixes em tanques-rede em um braço lateral do reservatório de Salto Caxias, Brasil. Foram selecionadas duas estações de amostragem no braço com tanques-rede e outras duas em um braço sem tanques. As variáveis abióticas e o biovolume fitoplanctônico não apresentaram diferenças significativas entre os locais estudados. Apenas mudanças temporais foram confirmadas pelas análises utilizadas. Os dois braços laterais foram classificados como oligotróficos, refletindo a baixa influência dos tanques-rede. O crescimento do fitoplâncton parece ter sido limitado principalmente por nitrogênio. Os valores de biovolume foram, em geral, baixos e cinco principais grupos funcionais foram observados (E, F, G, K e P). No verão, os maiores valores de biovolume foram observados e representantes de Chlorophyceae e Cyanobacteria dos grupos funcionais F e K, respectivamente, se destacaram. No inverno, o fitoplâncton foi composto principalmente por táxons de Bacillariophyceae do grupo P. O grupo G também foi restrito ao inverno e o grupo E ocorreu no inverno e verão. As variações registradas na estrutura do fitoplâncton parecem ter sido principalmente influenciadas pelas mudanças sazonais de temperatura, precipitação e disponibilidade de nutrientes. Os efeitos dos tanques-rede sobre as variáveis abióticas e biovolume fitoplanctônico parecem ter sido pequenos, provavelmente devido ao pequeno número de tanques utilizados e a capacidade de diluição do sistema. Entretanto, o monitoramento permanente do fitoplâncton é recomendado, uma vez que este ambiente possui uma capacidade de suporte, a partir da qual o estado trófico pode aumentar.
Subject(s)
Animals , Phytoplankton/physiology , Fisheries , Fishes/physiology , Food , Phytoplankton/classification , Phytoplankton/chemistry , Seasons , Brazil , Cyanobacteria/classification , Cyanobacteria/physiology , Cyanobacteria/chemistry , Diatoms/classification , Diatoms/physiology , Diatoms/chemistry , Conservation of Natural Resources , Biodiversity , Chlorophyta , NitrogenABSTRACT
Along the horizontal axis of reservoirs are generally recognized three zones (fluvial, transition and lacustrine) with distinct hydrodynamic and physical, chemical and biological properties. Quarterly samplings were conducted in 2002, in the limnetic region from each zone, at different depths of a tropical reservoir. To test the hypothesis that highest biomass (biovolume) of phytoplankton are found in the transition zone, the PERMANOVA analysis was realized. 106 taxa were recorded. Significant differences between biovolume values of the three zones were not verified (pseudo F = 0,89; p = 0,55). Higher values of biomass were obtained in lacustrine and transition zones of the reservoir. The functional group (FG) N (Cosmarium spp.) was dominant in these zones, in rain period, and related to low phosphorus concentration, high transparency and water column stability. Low biovolume values in reservoir characterized oligotrophic conditions in all zones of the reservoir, most of the period. The dominance of FGs Y, Lo, E, P and A, as evidenced by the CCA, was associated with low light availability and higher nutrients concentrations.
Ao longo do eixo horizontal de reservatórios são, em geral, reconhecidas três zonas (fluvial, transição e lacustre) com distinta hidrodinâmica e propriedades físicas, químicas e biológicas. Foram realizadas amostragens trimestrais no ano de 2002, na região limnética de cada zona, em diferentes profundidades, em um reservatório tropical. Para testar a hipótese de que os maiores valores de biovolume fitoplanctônico ocorrem na zona de transição do reservatório, foi realizada a Análise PERMANOVA. Foram registrados 106 táxons. Não foram registradas diferenças significativas entre os valores de biovolume fitoplanctônico das zonas do reservatório (pseudo F = 0,89; p= 0,55). Maiores valores de biovolume foram obtidos na zona lacustre e na zona de transição do reservatório. O grupo funcional N (Cosmarium spp.), dominante nestas zonas, no período chuvoso, esteve relacionado às baixas concentrações de fósforo, alta transparência e estabilidade da coluna de água. Os baixos valores de biovolume caracterizaram condições oligotróficas em todo o reservatório, na maior parte do período de estudo. A dominância dos FGs Y, Lo, E, P e A, como evidenciado por meio da CCA, esteve associada à baixa disponibilidade de luz e concentrações mais altas de nutrientes.
Subject(s)
Phytoplankton , Water ReservoirsABSTRACT
This study evaluated the influence of physical, chemical and physico-chemical variables of water on the biomass of periphyton community and verified the differences between six sampling sites over the course of São Mateus river: two upstream of the city of São Mateus, Espírito Santo State (E1, E2), two along (E3, E4), and two downstream of the city (E5, E6). The periphyton was collected from roots of Eichhornia crassipes (Mart.) Solms. Samplings were undertaken every week in September and October 2010. The periphyton biomass was estimated through chlorophyll 'a', biovolume, dry mass, ash-free dry mass, and ash. Higher values of chlorophyll 'a' were found at E1, while the total biovolume featured greater values in E4 and E3. Regarding the values of periphyton dry mass, the inorganic fraction was higher at sites along and downstream of the city of São Mateus. The variation of periphyton biomass was influenced by the availability of nutrients (phosphorus and nitrogen) and turbidity, as evidenced by the CCA. The results suggest that the input of allochthonous material, especially from human activities (fish farming and discharge of domestic and industrial wastewater), has changed the water quality (as pointed out by the PCA), as well as the communities present.
Este estudo avaliou a influência das variáveis físicas, químicas e físico-químicas da água sobre a biomassa da comunidade perifítica e verificou as diferenças entre seis estações amostrais ao longo do rio São Mateus: duas a montante da cidade de São Mateus, Estado do Espírito Santo (E1, E2), duas ao longo (E3, E4) e duas a jusante da cidade (E5, E6). O perifíton foi coletado de raízes de Eichhornia crassipes (Mart.) Solms. As amostragens foram realizadas em intervalos semanais, em setembro e outubro/2010. A biomassa perifítica foi estimada por meio da clorofila a, biovolume, massa seca, massa seca livre de cinzas e cinzas. Maiores valores de clorofila foram registrados em E1, enquanto o biovolume total apresentou valores mais elevados em E4 e E3. Quanto aos valores de massa seca perifítica, a parte inorgânica foi mais elevada, nas estações ao longo e a jusante da cidade. A variação da biomassa perifítica foi influenciada pela disponibilidade de nutrientes (fósforo e nitrogênio), assim como pela turbidez, como constatado pela CCA. Os resultados sugerem que a entrada de material alóctone, proveniente principalmente das atividades antrópicas (piscicultura e lançamento de efluentes domésticos e industriais) alteram a qualidade da água (como evidenciado na PCA), assim como as comunidades presentes.
Subject(s)
Biomass , Chlorophyll , PeriphytonABSTRACT
Biovolume calculation of micro algae is commonly used for the assessment in relative abundance (as biomass or carbon) of coexistent algae that vary in shape and/or size. Periphyton samples were obtained within a more general program, in fishery tanks and streams situated in the upper basin region of Tibagi River, nearby Londrina, Paraná state, south of Brazil. The present work proposes new algorithms for the determination of algal species volume. Results showed that Gyrosigma scalproides (Rabenhorst) Cleve 1894 and Tetraedron gracile (Reinsch) Hansgirg 1889 were exceptions inside their genus and the present new formula for biovolume and area gives more adequate results. In addition, new formulas applied for Phacus longicauda (Ehrenberg) Dujardin 1841 and Kirchneriella lunaris (Kirchner) Möbius 1894 also showed much more accurate results than in the previous works. The difference in the calculations of biovolume between the present method and others was not small and could not be neglected.
ABSTRACT
The resilience, ecological function and quality of both agricultural and natural systems were evaluated in the mountainous region of the Atlantic Rain Forest of Rio de Janeiro through Rapid Assessment Methods. For this goal new indicators were proposed, such as eco-volume, eco-height, bio-volume, volume efficiency, and resilience index. The following agricultural and natural systems have been compared according: (i) vegetables (leaf, fruit and mixed); (ii) citrus; (iii) ecological system; (iv) cattle, (v) silvo-pastoral system, (vi) forest fragment and (vii) forest in regeneration stage (1, 2 and 3 years old). An alternative measure (index) of resilience was proposed by considering the actual bio-volume as a function of the potential eco-volume. The objectives and hypotheses were fulfilled; it is shown that there does exist a high positive correlation between resilience index, biomass, energy efficiency and biodiversity. Cattle and vegetable systems have lowest resilience, whilst ecological and silvo-pastoral systems have greatest resilience. This new approach offers a rapid, though valuable assessment tool for ecological studies, agricultural development and landscape planning, particularly in tropical countries.
Foram avaliadas, em região montanhosa da Mata Atlântica do Rio de Janeiro a resiliência, função ecológica e qualidade tanto do sistema agrícola como natural, através dos métodos de avaliação rápida ("rapid assessment methods"). Para este fim, foram propostos novos indicadores como eco-volume, eco-altura, bio-volume, eficiência volumétrica e índice de resiliência. Os seguintes sistemas agrícolas e naturais foram comparados: (i) hortaliças (folhas, frutos e mistos); (ii) citros; (iii) sistema ecológico; (iv) gado; (v) sistema silvo-pastoral; (vi) fragmento florestal; (vii) floresta em estágio de recuperação (1, 2 e 3 anos de idade). Uma forma alternativa de resiliência foi proposta considerando o bio-volume real como uma função do eco-volume potencial. Os objetivos e hipóteses foram alcançados; demonstrou-se que existe uma correlação altamente positiva entre índice de resiliência, energia da biomassa, eficiência energética e biodiversidade. Pecuária e sistemas de hortaliças apresentaram as mais baixas resiliências enquanto sistemas ecológico e silvo-pastoral tiveram maiores resiliências. Esta nova estratégia oferece uma rápida e valiosa ferramenta de avaliação para estudos ecológicos, desenvolvimento agrícola e planejamento paisagístico, especialmente em países tropicais.