ABSTRACT
Objective To study the chemical constituents of the fruits of Xylocarpus granatum. Methods The isolation and purification were carried out by silica gel column chromatography, Sephadex, preparative TLC, and preparative HPLC. The structures of the isolated compounds were identified with the help of HR-ESI-MS, 1H-NMR, 13C-NMR, H-H COSY, HMQC and HMBC techniques. Results Seventeen compounds were isolated and elucidated from the fruits of X. granatum, their structures were identified as: grantumin C (1), cipadesin A (2), xylocarpin G (3), febrifugin (4), tigloylseneganolide A (5), khaysin T (6), cipadesin (7), granatumin B (8), xyloccensin S (9), granaxylocarpin C (10), xylogranatin E2 (11), xyloccensin Q (12), xyloccensin P (13), cedrodorin (14), xylorumphiins D (15), hydroxydammarenone-II (16), and bis (2-ethylhexyl) phthalate (17). Conclusion Compounds 2, 7, 14, 16 and 17 are isolated from the plants of Xylocarpus Koenig for the first time. Compound 15 is obtained from X. granatum for the first time.
ABSTRACT
Objective To develop a method for the determination of DBP and DEHP in cosmetics by GC-MS. Methods The samples was extracted by methanol or acetic ether, DBP and DEHP were separated by GC and determined by MS. Results The linear range, regression equation of calibration curve and correlation coefficient were 0.5-100.0 ?g/ml, y=2?106 x-2?106, 0.999 6 for DBP respectively and 5.5-110.0 ?g/ml, y=988 223 x-7?106, 0.995 9 for DEHP respectively. Based on threefold ratio of signal and noise, as the sample was 1.0 and 0.2 g respectively, the volume was 10.0 ml, the detection limits were 1.0 and 5.0 mg/kg respectively for both DBP and DEHP. The recovery rates were 90.8%-119.0% for DBP and 90.4%-115.3% for DEHP. Relative standard deviations were 4.8%-9.8% for DBP and 6.0%-8.6% for DEHP. Conclusion This method is sensitive, accurate and high reproducible, and was applicable to the determination of DBP and DEHP in the cosmetics.