ABSTRACT
Background:Bixa orellanaLinn, (Family: Bixaceae) commonly known as “Lipstick tree” have been extensively used traditional medicine in India and others part of the world to cure laxative, cardiotonic, hypotensive,expectorant, antibiotic, antipyretic, aphrodisaic etc. It has been found that B. orellanacontains different phytochemical groups such as phenolic compounds, glycosides, tannins, steroids, alkaloids, saponins.The project work has been designed to investigate the phytochemical nature (group determination of plant constituents), analgesic and neuropharmacological activity of B.orellanaleaves extract. Methods:Analgesic activity of B. orellanawas determined by acetic acid induced writhing, hot plate, tail immersion and paw tickling test/formalin induced nociceptortest. Open field, hole cross, tail suspension and light/dark box test were employed for the assessment of neuropharmacological activity of B. orellana.Results:Phytochemical screening revealed the presence of reducing sugar, alkaloids, glycosides, gum,terpenoids, tannins, steroids and flavonoids. In analgesic activity test, the sample showed significant analgesic activity. The dose-dependent neuropharmacological activity is shown in neuropharmacological activity test.Conclusions:Our exploration suggests that B. orellanacontains bioactive compounds and it should be studied further for isolation and purification of such novel compounds
ABSTRACT
Bixa orellana is used in popular medicine against several diseases. Extracts of its fruit, root and leaf presented antimicrobial activity, while seed extract showed negative results. This study aimed at verifying the antimycobacterial activity of B. orellanalyophilized hydroalcoholic extracts over Mycobacterium tuberculosis, and determine the Minimum Inhibitory Concentration against five bacteria. Antimycobacterial activity was determined by diffusion technique, while MIC was assessed by diffusion and colorimetric analysis. MICs were 0.3, 0.5 and 0.2 mg/mL respectively, for leaf, root and stem extracts, against M. tuberculosis. Stem's extract showed 1.2 mg/mL for B. cereus, 1.53 mg/mL for S. aureusand S. typhimurium, 4.50 mg/mL for P. aeruginosaand 8.01 mg/mL for P. mirabillis.Leaf extracts showed 0.66 mg/mL for P. aeruginosa, 0.94 mg/mL for P. mirabillis, 1.88 mg/mL for S. aureus, 3.95 mg/mL for B. cereusand 8.37 mg/mL for S. typhimurium. Root's extracts showed 0.25 mg/mL for P. aeruginosa,0.31 mg/mL for S. aureus, 0.62 mg/mL for S. typhimuriumand 3.00 mg/mL for B. cereusand P. mirabillis. Leaf and stem extracts showed antimycobacterial activity. MICs were lower in colorimetric analysis than in agar diffusion. Extracts revealed bacteriostatic activity against the five bacterial.
Bixa orellana é usada na medicina popular em várias doenças. Extratos do fruto, raiz e folhas apresentaram atividade antimicrobiana, enquanto extratos do caule mostraram resultados negativos. Este estudo teve por objetivo verificar a atividade antimicobacteriana de extratos hidroalcoólicos liofilizados de B. orellanafrente Mycobacterium tuberculosis e determinar a Concentração Inibitória Mínima para cinco bactérias. A atividade antimicobacteriana foi determinada por difusão e a CIM por difusão e análise colorimétrica. As CIM foram 0,3, 0,5 e 0,2 mg/mL para os extratos de folhas, raiz e caule frente M. tuberculosis. Os extratos de caule demonstraram CIM de 1,12 mg/mL (B. cereus), 1,53 mg/mL (S. aureuse S. typhimurium), 4,50 mg/mL (P. aeruginosa) e 8,01 mg/mL (P. mirabillis); extratos de folhas mostraram 0,66 mg/mL (P. aeruginosa), 0,94 mg/mL (P. mirabillis), 1,88 mg/mL (S. aureus), 3,95 mg/mL (B. cereus) e 8,37 mg/mL (S. typhimurium); extratos de raiz demonstraram 0,25 mg/mL (P. aeruginosa), 0,31 mg/mL (S. aureus), 0,62 mg/mL (S. typhimurium) e 3,00 mg/mL (B. cereuse P. mirabillis). Extratos de folhas e caule mostraram atividade antimicobacteriana. As CIM por colorimetria foram menores que na difusão em agar. Os extratos revelaram atividade bacteriostática sobre as cinco espécies bacterianas.
ABSTRACT
Neste trabalho foi verificado o teor de Óleos, sólidos e bixina em sementes de quatro variedades de Bixa orellana L. cultivadas na Paraíba: "Casca verde", "Casca vermelha", Bico de calango" e "Grão preto". Os melhores resultados foram obtidos com os tipos "Casca verde" e "Casca vermelha" que apresentaram um rendimento em bixina pura, cristalina, de 1,3 e 1,1 por cento, respectivamente.
In this work it was verified the content of oils, solids and bixin in seeds of four varieties of Bixa orellana L. cultivated in Paraíba: "Casca verde", "Casca vermelha", Bico de calango" and "Grão preto". The best results were obtained with the types "Casca verde" and "Casca vermelha" that presented an yield in pure, crystalline bixina, of 1,3 and 1,1 percent, respectively.