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Objective To study the effects of Lentinan on bleomycin A5’s inhibition effects in hemangioma-derived endothelial cell. Methods Hemangioma-derived endothelial cell line EOMA were divided into LTN group (only lentinan treatment), BLE group (only bleomycin A 5 treatment), L+B group (Lentinan and bleomycin A5 treatment) and CON group (no lentinan and bleomycin A5 treatment). Cell proliferation, cell cycle, PI and apoptosis were detected and compared among four groups. Results The differences of absorbance in LBE group was significantly higher than that in L+B group after treatment for d 1-d 7 (P<0.05), but both of them were significantly lower than in CON group and LTN group (P<0.05). The G 0/G 1 stage and apoptosis rate in L+B group was significantly higher than in BLE group(P<0.05), while it was significantly lower in S stage, G 2/M stage and PI(P<0.05). Conclusion Lentinan could significantly promote bleomycin A5’s inhibition effects on hemangioma-derived endothelial cell.
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ObjectiveTo evaluate and compare the curative effect and the safety of sclerotherapy of venous malformation in children using absolute ethanol and pingyangmycin.MethodsEighty children with venous malformation were treated by sclerotherapy with absolute ethanol or pingyangmycin under general anesthesia during February of 2009 to May of 2011. Diagnostic criteria included the presence of a bluecolored lesion at birth,with gradual increase in its size associated with a positive postural test.MRI showed characteristic imaging features.Eighty patients were divided into two groups by randomization.Forty patients were treated with absolute ethanol,and the remaining 40 patients with pingyangmycin. The therapeutic effects and side effects were observed and recorded during a follow-up period of 6 to 24 months ( average 15 months).Treatment results weredividedinto four categories: cure, basiccure, effective, and ineffective,and the former three categories were considered effective in treatment.Chi-square test was used to compare the rate of effective treatment and rate of complications in these two groups.ResultsThe effective patients treated with absolute ethanol was 38 (95.0％,38/40),and the effective patients with pingyangmycin was 26(65.0％,26/40),x2 test ( x2 =11.25,P ＜ 0.01 ) difference was statistically significant.Eight patients developed skin necrosis; 4 patients serious local swelling,2 patients muscle fibrosis,1 patient cerebral embolism in the absolute alcohol group.Two patients developed postoperative fever and vomiting and 2 skin necrosis in the pingyangmycin group,the difference was statistically significant (x2 =12.29,P ＜0.01 ). ConclusionsSclerotherapyiseffectiveforthetreatmentofvenousmalformationsin children.Absolute ethanol is more effective,but associated with more complications,such as skin necrosis or as serious as cerebral embolism,than pingyangmycin.
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Purpose:To study the methods of treatment of spinalcord implanation medulloblatoma with combined chemotherapy. Methods:Teniposide (VM 26) 100 mg/day (dissolving in 250 ml physiological saline(N.S.)) was injected intravenously in 3 consecutive days. And it was repeated after a 7 day interval, and bleomycin A 5(PYM) 4 mg (in 250 ml N.S.) was intravenously injected twice a week. Methotrexate (MTX) 10 mg (in 5 ml N.S.) and dexamethsone 3 mg were injected intrathically once a week. The whole treatment included 5 courses and lasted about one and half month.Results:The study involved 6 cases. MRI, whick were performed 2 weeks after the treatment, showed tumors disappeared in 2 cases and 80% shrunk in 4 cases. The tumors completely disappeared after repeated treatments in the 4 cases. Conclusions:Combined chemotherapy is an efficient alternative treatment for spinalcord implanation medulloblatoma.
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objective To evaluate the value of HRCT on differential diagnosis of a rat model of pulmonary fibrosis induced with bleomycin A5 and to replicate a rat model of pulmonary fibrosis for radio imaging study to investigate pathogenetic mechanism of pulmonary fibrosis. Methods In 50 of 60 male Sprague Dawley rats bleomycin A5 was given through the trachea, and the other 10 received same volume of normal saline. After 28 days, HRCT and pathological examination of the lungs were performed, and the changes were correlatively studied. Results HRCT showed pulmonary fibrosis in all the 50 rats treated with bleomyein, including ground glass opacity, nodular shadow, abnormality in bronchio vasarlar linings, pleural thickening, and in some with beehive like changes. Lung fibrosis was corroborated by pathological sections. Conclusion HRCT can be used to diagnose pulmonary fibrosis of living rats. It is a simple, accurate, reliable, and it can give good representation.