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There is considerable heterogeneity in the peripheral blood smear reports across different diagnostic laboratories, despite following the guidelines published by the International Council for Standardization in Haematology (ICSH). As standardization of reports can facilitate communication and consequently the diagnostic efficiency in both laboratories and clinics, the standardization committee of the Korean Society for Laboratory Hematology aimed to establish a detailed guideline for the standardization of peripheral blood smear reports. Based on the ICSH guidelines, additional issues on describing and grading the peripheral blood smear findings were discussed. In this report, the proposed guideline is briefly described.
Subject(s)
Blood Cells , Hematology , Population CharacteristicsABSTRACT
Two trials were conducted with proficiency tests for complete blood cell count (CBC) and blood cell morphology as part of the 2018 Routine Hematology Program of the Korean Association of External Quality Assessment Service. Three different control samples were sent for CBC testing and two blood cell morphology pictures were posted on the laboratory website during each trial. The mean response rates of the 1,719 participating laboratories were 97.4% and 37.2% for CBC and blood cell morphology, respectively. The distribution of equipment for CBC testing was comparable to that of the previous year. The coefficient of variation (CV) ranges were determined as 3.5%–4.1%, 1.9%–2.7%, 1.4%–2.8%, 4.5%–5.3%, and 5.4%–6.9% for white blood cell counts, red blood cell counts, hemoglobin, hematocrit, and platelet counts, respectively. The concordance rate ranged from 83.0% to 97.5% in blood cell morphology tests. We observed a continuous increase in the number of participating laboratories and a trend towards a decrease in the CVs of platelet counts compared to those in 2016. Values of the other assessed parameters were similar to those of the previous year.
Subject(s)
Blood Cell Count , Blood Cells , Erythrocyte Count , Hematocrit , Hematology , Laboratory Proficiency Testing , Leukocyte Count , Platelet CountABSTRACT
Under the Routine Hematology Program of the Korean Association of External Quality Assessment Service, complete blood count (CBC) and blood cell morphology proficiency tests were performed during 2017. We conducted two trials, sent three control materials for CBC testing, and posted two cell morphology pictures on the website for each trial. Of 1,646 laboratories, the mean response rates for CBC and blood cell morphology were 96.7% and 42.0%, respectively. The distribution of equipment for CBC testing was similar to that of the previous year. The coefficient of variations for white blood cell counts, red blood cell counts, hemoglobin, hematocrit, and platelet counts were 3.7%–4.7%, 2.2%–2.4%, 1.5%–3.0%, 5.4%–6.0%, and 7.2%–10.2%, respectively. More than 80% of the blood cell morphology tests were concordant among all of the trials, except for the HHI-17-03 trial. In 2017, the number of participating laboratories increased, and the performance was similar to that of the previous year.
Subject(s)
Blood Cell Count , Blood Cells , Erythrocyte Count , Hematocrit , Hematology , Korea , Laboratory Proficiency Testing , Leukocyte Count , Platelet CountABSTRACT
Under the Routine Hematology Program of Korean Association of External Quality Assessment Service, proficiency tests for complete blood count (CBC) and blood cell morphology were performed during 2016. We conducted two trials, and sent three level control materials for CBC testing and posted two cell morphology pictures on the website for each trial. Of 1,455 laboratories, the mean response rates for CBC and blood cell morphology were 96.3% and 40.4%, respectively. The distribution of equipment for CBC test was similar to that of previous year. The coefficient of variations for white blood cell counts, red blood cell counts, hemoglobin, hematocrit, and platelet counts, were 3.8%–4.5%, 1.9%–2.3%, 1.4%–2.8%, 4.8%–5.8%, and 6.2%–9.4%, respectively. More than 80% of the blood cell morphology tests were concordant in all trials. In 2016, the number of participating laboratories increased, and the performance was similar to that of the previous year.
Subject(s)
Blood Cell Count , Blood Cells , Erythrocyte Count , Hematocrit , Hematology , Korea , Laboratory Proficiency Testing , Leukocyte Count , Platelet CountABSTRACT
During 2015, the Diagnostic Hematology Subcommittee of Korean Association of External Quality Assessment Service performed laboratory proficiency testing for blood cell count, cell morphology, and coagulation tests. Four trials for blood cell count and cell morphology tests each and two trials for coagulation tests were performed. The trials for blood cell counts had a reply rate of 97.2% among 1,352 laboratories, compared to 99.0% among 503 laboratories for cell morphology and 98.6% among 574 laboratories for coagulation tests. The homogeneity of the external quality materials was stable (80% for most of the participating laboratories. The CVs for the coagulation tests varied according to the specific instruments or reagents that were used. An educational workshop was held in November to provide hands-on experience in diagnostic hematology. During 2015, the number of participating laboratories increased, while the performance of hematology tests was similar to that observed in the previous year.
Subject(s)
Blood Cell Count , Education , Erythrocyte Count , Hematocrit , Hematology , Indicators and Reagents , Korea , Laboratory Proficiency Testing , Leukocyte Count , Partial Thromboplastin Time , Platelet Count , Prothrombin TimeABSTRACT
Objective To investigate the morphological characteristics of peripheral blood red blood cell (RBC) in the patients with anemia .Methods The peripheral blood smears were selected from 93 cases of anemia and performed the morphological exami‐nation by the automatic reading instrument ,the heteromorphic red blood cells were examined by the manual microscopy .Results Among 93 patients of anemia analyzed by the DM‐96 automatic blood cell morphological analytical system ,anisocytosis accounted for 77 .4% ,in which 48 .4% were mainly microcytic ,heteromorphic RBC accounted for 55 .9% ,the detection rate of polychromato‐phil RBC had large difference between the manual microscopy and the instrument automatic reading ,the hypochromic RBC accoun‐ted for 11 .8% .The top three places of detection rates in heteromorphic RBC were elliptocyte ,RBC fragment and RBC rouleaux ar‐rangement .Conclusion Peripheral blood erythrocyte morphology analysis has an important significance in the RBC diseases ,espe‐cially in the diagnosis ,prognosis and treatment of anemia ,which provides the important basis of morphology for the reliability of la‐boratory report .
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Objective To evaluate the value of detecting abnormal lymphocyte morphology in peripheral blood and Epstein Barr Virus(EBV)DNA quantity through real-time quantitative PCR (RT-qPCR)in the initial diagnosis for infants patients with infectious mononucleosis (IM).Methods From Jan.2013 to Dec.2014 212 infants patients with IM were analysed ret-rospectively,which were all in-patients in the hospital.The abnormal lymphocyte morphology in peripheral blood and Epstein Barr Virus (EBV)DNA quantity were both detected in the initial fever period and a week later.The latter was detected by real-time quantitative PCR (RT-qPCR),combined with all the symptoms were all analysed comprehensively.The percentage of abnormal lymphocyte more than 10% was positive,and the EBV-DNA quantity more than 1.0×103 copy per ml was posi-tive,too.Results Of all the infants patients,in the initial fever period,82 patients had more than 10% positive abnormal lymphocyte and 100 patients had positive EBV-DNA quantity.But a week later,156 patients had more than 10% positive ab-normal lymphocyte,the maximum abnormal lymphocyte was 56%.And 180 patients had positive EBV-DNA quantity.When both abnormal lymphocyte morphology in peripheral blood and Epstein Barr Virus (EBV)DNA quantity were detected,in the initial fever period,125 patients were positive,it rose significantly more than that of abnormal lymphocyte morphology in peripheral blood (χ2=17.45,P0.05).Conclusion The detecting of peripheral blood cell morphology combined with EBV-DNA quantity are very important in the initial diagnosis for infants patients with infectious mononucleosis.Including all the symp-toms,they could improve the diagnosis timely and accurately.
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Objective To compare the difference of peripheral blood leucocyte percentages in malaria patients among Sysmex‐XE5000 ,CellaVisionDM96 and manual microscope classification ,and to verify the accuracy and reliability of the Sysmex‐XE5000 automatic blood corpuscle detection instrument for detecting the leukocyte classification in blood routine .Methods The leucocyte percentages data in 82 cases of malaria detected by using the Sysmex‐XE5000 in the Shenzhen Municipal Third People′s Hospital from January 2011 to December 2015 were retrospectively collected ;the peripheral blood smear in 82 cases of malaria obtained the percentages after the classification by the CellaVisionDM 96 ;then the peripheral blood smear was performed the leucocyte classifica‐tion by the manual microscopy for calculating the percentage .Results In the pairwise comparison of percentage obtained from the peripheral blood leucocyte classification by Sysmex‐XE5000 ,CellaVisionDM96 and manual microscopy ,only the monocytes percent‐age had statistical difference between CellaVisionDM 96 and manual microscopy (P0 .05) .Conclusion By comparing the peripheral blood leucocyte percentages data in malaria patients by Sysmex‐XE5000 ,CellaVisionDM96 and manual microscopic classification ,it is indicated that the leukocyte classification data by Sysmex‐XE5000 are accurate and reliable ,malaria parasite does not affect peripheral blood leukocyte classification ,but it is necessary to pay more attention to monocytes classification in CellaVision DM 96 classification .
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To improve the effect of teaching practice of blood cell morphology,typical cases of peripheral blood erythrocyte,granulocyte and platelet were chosen,case show and problem-based teaching way were applied to encourage students to find information of autonomous learning.Then teacher organized the class discussion,and the information was analyzed and summarized.In the implementation of the case teaching,we should pay attention to improving students' learning initiative.At the same time,teachers need to have knowledge,comprehensive ability and ability in organization and leadership,to promote students' active participation in discussion in the teaching,pay attention to the information feedback,and improve the teaching details
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Hematology and blood cell cytochemistry of Rhinella fernandezae (Amphibia: Anura) from Espinal and Delta-Islands of Paraná River, Argentina. The description of amphibian hematology is scarce and most of these studies have been done in species from North America, Asia and Europe. With the purpose to obtain basic hematological information of Rhinella fernandezae, 23 blood samples from Santa Fe and Entre Ríos natural reserves were studied. Blood of each individual was extracted by cardiac puncture and hemograms were carried out. Morphological and cytochemical description of blood cells were analyzed in slides and were inspected for extra and intra cellular parasites. Five leucocytes types were observed, being lymphocytes the predominant ones followed by basophiles. Heterophils and eosinophils were positive to PAS, Sudan B and peroxidase. The erythrocytes and its precursors were negative for cytochemical reactions. Micronuclei and nuclear alterations frequencies were scarce. No significant differences (p>0.05) were observed between sexes neither in hemograms nor in blood cells morphology. Microfilarias were the only hemoparasites found with a relative low prevalence and infection intensity. The hematological characteristics studied were similar to those reported for other amphibians, suggesting that R. fernandezae individuals present optimal nutritional and immunological status. Rev. Biol. Trop. 59 (1): 17-28. Epub 2011 March 01.
La descripción de la hematología de los anfibios anuros es escasa, habiéndose realizado la mayoría de los trabajos en especies de Norteamérica, Asia y Europa. Con el propósito de obtener datos hematológicos para Rhinella fernandezae, fueron estudiados 23 especímenes provenientes de zonas protegidas de las provincias de Santa Fe y Entre Ríos. Se les extrajo sangre por punción cardíaca y se realizaron hemogramas. En los extendidos sanguíneos, se efectuaron la descripción morfológica y citoquímica de las células sanguíneas y búsqueda de parásitos. Se observaron cinco tipos de leucocitos, donde predominaron los linfocitos pequeños. Heterófilos y eosinófilos resultaron positivos para PAS, Sudan B y peroxidasa; contrariamente, los eritrocitos y sus precursores fueron negativos. Los puntajes de las reacciones citoquímicas fueron variables para basófilos, linfocitos, monocitos y trombocitos. Las frecuencias de micronúcleos y de alteraciones nucleares fueron escasas. No se observaron diferencias significativas (p>0.05) entre sexos en el hemograma ni en la morfología sanguínea. Los únicos hemoparásitos encontrados fueron microfilarias (Nematoda: Filaroidea), cuya prevalencia e intensidad de infección fueron bajas. Las características hematológicas estudiadas fueron semejantes a los valores reportados para otros anfibios, pudiendo inferir que los individuos de R. fernandezae estudiados se encuentran en un buen estado nutricional e inmunológico.
Subject(s)
Animals , Female , Male , Anura/blood , Blood Cells , Hematologic Tests/veterinary , Histocytochemistry/veterinary , Argentina , Anura/parasitology , Blood Cells/chemistry , Blood Cells/cytology , Blood Cells/parasitology , Reference Values , RiversABSTRACT
Morphological examination of blood cells is an important part of the hematology examination course. In order to enrich teaching resources, network of bilingual education resource was established and put into application by the Department of Hematology in Guangzhou Medical College. The repository improved teaching quality of cell morphology, and played a role in training personnel of hematology examination with solid basic skills.