ABSTRACT
Objective To evaluate Persicae Semen-Rhei Radix et Rhizoma (PS-RRR) of different compatibility of acute blood stasis rats blood rheology and blood coagulation function, and to reveale the effect of PS-RRR for promoting blood circulation to remove blood stasis effect scope, nature, and degree of interaction. Methods With ice water bath and injected adrenaline hydrochloride copy of acute blood stasis rats model, different ratio (0:1, 1:5, 2:5, 2:3, 1:1, 3:2, 5:2, 5:1, and 1:0) of different concentration of PS-RRR was given for later. Through the determination of whole blood viscosity (WBV), blood sedimentation (ESR), erythrocyte aggregation index (EAI), prothrombin time (PT), partial thrombin (APTT), fibrinogen (FIB) content of coagulation, thrombin time (TT), and the blood rheology of blood stasis rats was observed, the influence of the blood coagulation indexes. Then response surface analysis and multi-index comprehensive index method of PS-RRR different compatibility of promoting blood circulation was used to remove blood stasis effect comprehensive comparative analysis. Results The ratio of PS-RRR between 2:3 to 3:2 showed obvious synergy (strength of synergy: -0.8); In PS dose from 5.5-10 g and RRR dose from 2.1-5.8 g area showed the antagonism function (antagonism effect strength maximum: 0.6); While other percentage did not show obvious synergy or antagonism. Conclusion The results of reveal that scope, nature, and degree of PS-RRR for promoting blood circulation to remove blood stasis interaction effect, the and its prescriptions of traditional Chinese medicine (TCM) and clinical use of PS-RRR 1:1 is consistent with the highest frequency of conclusion. PS-RRR provides scientific basis for clinical applications.
ABSTRACT
Objective: To evaluate the activating blood circulation and dissipating blood stasis effects of the compatibility between two effective parts (DT, DF, DST, and DF + DST) of Salvia miltiorrhiza. with different doses on rats with acute blood stasis. Methods: Acute blood stasis rat model was induced by sc injection of adrenaline and ice water bath. The blood stasis mice were ig administered with different effective parts of S. miltiorrhiza. The whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentation rate (ESR), and haematocrit (HCT) were determined to observe the effects of two effective parts of Danshen on hemorheology of blood stasis rats. And the thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and plasma fibrinogen (FIB) were tested to observe the effects of different effective parts of S. miltiorrhiza on blood coagulation function of blood stasis rats. In addition, the maximum aggregation induced by adenosine diphosphate (ADP) was tested to observe the effect of different active parts of S. miltiorrhiza on platelet aggregation index of blood stasis rats. Then principal component analysis (PCA) and multi-attribute comprehensive index methods were both used to comprehensively evaluate the total activating blood circulation and dissipating blood stasis effects of different effective parts of S. miltiorrhiza. Results: The hemorheological indexes and coagulation parameters of model group had the significant differences with normal group. Compared with model group, the effective parts of S. miltiorrhiza with different doses could improve the blood hemorheological indexes and coagulation parameters in acute blood stasis rats with dose-effect relation. Based on the PCA and multi-attribute comprehensive index methods, the group of high-dose DF + DST had the best effect of activating blood circulation and dissipating blood stasis effect in all medication administration teams. Under the condition of same dose of the effective parts of S. miltiorrhiza, the DF + DST could obviously improve the hemorheology and blood coagulation function in acute blood stasis rats. Conclusion: The combination of DF and DST has the better effects of activating blood circulation and dissipating blood stasis in comparison with those of single part. It could provide the scientific basis for more effective application of the compatibility between salvianolic acids and tanshinones in modern clinic medicine.