ABSTRACT
Objective To study the protective effect of blueberry anthocyanins on H2O2 induced human umbilical vein endothelial cells,HUVECs.Methods The cultured cells were divided into 3 groups:normal control group,H2O2 model group,blueberry anthocyanins group.MTT method was used to observe cell viability.Annexin V-FITC/PI straining was used to observe cell apoptosis.Flow cytometry was used to detect cell cycle.Results H2O2could induce HUVECs oxidative stress injury.Cell survival rate was positive correlated with drug concentration and action time.In the certain concentration range blueberry anthocyanin had a protective effect.Conclusion Blueberry anthocyanin can protect H2O2induced oxidative stress injury in HUVECs.
ABSTRACT
Objective To investigate the anti-fatigue activity of fermented grain-containing blueberry anthocyanins ( LANHE,LH) in mice.Methods Experiments were conducted in two phases .In the first phase , forty mice were randomly divided into four groups:control group(distilled water,dw) and three LH administration groups (8.75,17.5 and 35.08 ml/kg body mass).In the second phase, mice were randomly divided into two groups:high-dose LH group (35.08 ml/kg body mass) and control group (dw 35.08 ml/kg body mass).After four weeks, a forced swimming test was performed and the biochemical parameters related to fatigue were examined .Results and Conclusion The administration groups showed a significant increase of swimming time to exhaustion compared with the control group , especially the high-dose group ( P0.05).LH could significantly increase the liver glycogen contents and decrease the serum contents (P<0.05).These data indicate that LH has anti-fatigue activity and can elevate the exercise tolerance in mice .