ABSTRACT
The present study was aimed to evaluate the antibacterial capability of fucoidan from Sargassum wightii against the chosen human bacterial pathogens. The major chemical constituents of the extracted fucoidan were analyzed by biochemical methods. It showed that the extracted fucoidan contains 52.86 ± 0.64% of fucose and 29.26 ± 0.83% of sulphate. The antibacterial efficacy was performed by agar well diffusion, minimum inhibitory concentration (MIC) and minimum inhibitory concentration (MBC) method. The maximum antibacterial activity 18.6 ± 0.32 mm was obtained for Vibrio cholera and the minimum activity 8.6 ± 0.26 mm was obtained for Salmonella typhi. Result of this manifested the considerable antibacterial potentiality of fucoidan against human bacterial pathogens. Toxicity of fucoidan was evaluated by brine shrimp toxicity assay. No toxic effect was observed in fucoidan. Our study concluded that fucoidan might be used as natural and safe antibiotics in curing many bacterial diseases. Further study is required to get the better understanding of mode of action of fucoidan against the bacterial pathogens.
ABSTRACT
The present study was aimed to evaluate the antibacterial capability of fucoidan from Sargassum wightii against the chosen human bacterial pathogens. The major chemical constituents of the extracted fucoidan were analyzed by biochemical methods. It showed that the extracted fucoidan contains 52.86 ± 0.64% of fucose and 29.26 ± 0.83% of sulphate. The antibacterial efficacy was performed by agar well diffusion, minimum inhibitory concentration (MIC) and minimum inhibitory concentration (MBC) method. The maximum antibacterial activity 18.6 ± 0.32 mm was obtained for Vibrio cholera and the minimum activity 8.6 ± 0.26 mm was obtained for Salmonella typhi. Result of this manifested the considerable antibacterial potentiality of fucoidan against human bacterial pathogens. Toxicity of fucoidan was evaluated by brine shrimp toxicity assay. No toxic effect was observed in fucoidan. Our study concluded that fucoidan might be used as natural and safe antibiotics in curing many bacterial diseases. Further study is required to get the better understanding of mode of action of fucoidan against the bacterial pathogens.
ABSTRACT
O presente trabalho descreve os resultados do estudo farmacognóstico, estudo fitoquímico preliminar, toxicidade em Artemia salina e atividade antiplasmódica contra cepa de Plasmodium falciparum resistente a cloroquina (W2) do extrato etanólico obtido do pó das folhas de Montrichardia linifera (Arruda) Schott, Araceae. Para realização dos estudos farmacognósticos utilizou-se as metodologias descritas na Farmacopéia Brasileira. A prospecção fitoquímica foi realizada pelos métodos descritos por Mattos. No ensaio antiplasmódico foi utilizado o microteste tradicional e no ensaio de Artemia salina. Os resultados obtidos nos estudos farmacognósticos do pó das folhas de M. linifera demonstraram tratar-se de um pó moderadamente grosso, com teores de água e cinzas totais dentro dos limites aceitáveis, baixa densidade (0,324 e 0,339 g/mL), pH próximo de neutro (6,69±0,02). A análise fitoquímica preliminar evidenciou a presença de alcaloides, flavonoides, taninos, triterpenos e esteroides. O extrato etanólico das folhas de M. linifera apresentou moderada atividade antiplasmódica e baixa toxicidade para a Artemia salina.
The present paper describes results of a pharmacognostic, a preliminary phytochemical study, brine shrimp toxicity and antiplasmodic activity in a strain of Plasmodium falciparum that is resistant to chloroquine (W2) using ethanolic extract obtained from powder of the leaves of Montrichardia linifera (Arruda) Schott, Araceae. To perform the pharmacognostic studies methodologies employed in the Brazilian Pharmacopoea were utilized. The methods described by Mattos were utilized for phytochemical prospecting. In the antiplasmodic test the traditional microtest was employed, as well as in the test with brine shrimp. The results obtained in the pharmacognostic studies with the powder from M. linifera leaves showed that it was a moderate thick powder, with water and ash totals within acceptable limits, low density (0.324 and 0.339 g/mL), pH close to neutral (6.69 ± 0.02). Preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins, triterpenes and esters. The ethanolic extract from M. linifera leaves presented moderate antiplasmodic activity and low brine shrimp toxicity. To summarize, the powder used in preparing the extract presented good quality in pharmacognostic terms.