ABSTRACT
The present study was attempted to evaluate diuretic potency of total alcoholic extracts and its polar and non polar fractions of Priyala fruits (Buchanania angustifolia, Buchanania lanzan Fam: Anacardiaceae) in rats. Healthy adult Wistar albino rats weighing about 150-200 g were used for the study. Acute toxicity study was performed to determine therapeutic dose of the extracts and fractions. Lipschitz method was employed for the assessment of diuretic activity, the control group received normal saline (25ml/kg P.O), and standard group received Frusemide (20mg/ kg, P.O). The remaining groups were treated with two doses (250 and 500mg/kg) of Buchanania angustifolia and Buchanania lanzan total alcoholic extracts, n-hexane insoluble and n-hexane soluble fractions. Total alcoholic extracts, n-hexane insoluble and n-hexane soluble fractions of the both fruits have shown significant diuresis when compared with control at 5 hrs. Out of two doses 500mg/kg has shown significant diuretic property resulting in the superior urine excretions of Na+ and K+ ions which can be compared to that of standard. With the same dose Buchanania angustifolia was found to be better diuretic than Buchanania lanzan. Priyala fruit extracts and n-hexane fractions produced significant diuretic effect at a dose of 500 mg/kg which appeared to be comparable with that of the standard drug Frusemide. However, further studies are encouraged to isolate the active phytochemical constituent for exploring exact mechanism of diuresis.
ABSTRACT
Objective:To evaluate the wound healing activity of the methanolic root extract of Buchanania lanzan Spreng. (B. lanzan), with a focus on antimicrobial and anti-biofilm properties. Methods: The extract was evaluated for its wound healing properties (excision and incision models) as evident from the analysis of tensile strength and wound contraction. The extract was also screened for antibacterial properties against different Gram positive and Gram negative bacterial strains. B. lanzan was also studied for its effect on biofilm formation and disruption of preformed biofilms. The synergistic effect of B. lanzan was determined in combination with gentamicin. Results:Topical application of B. lanzan (10%w/w ointment) significantly increased (40.84%) the tensile strength in the incision wound model. B. lanzan also showed significant wound healing activity in excision model and such significant activity was observed from the 9th day. Whereas Soframycin displayed significant wound healing activity from the 6th day. It was found that root extracts of B. lanzan revealed significant inhibition against all tested pathogens. B. lanzan displayed antimicrobial activity against Gram positive (MIC 0.625 mg/mL) and Gram negative (MIC 0.625-1.25 mg/mL). B. lanzan was able to reduce biofilm formation and also caused disruption of preformed biofilms in a manner similar to ciprofloxacin. However, gentamicin was found to be ineffective against biofilms formed by Gram negative organism. According to the fractional inhibitory concentration index, B. lanzan displayed synergistic activity when it was combined with gentamicin. Conclusions:From this study it may be concluded that the root extract of B. lanzan revealed significant wound healing potential, which was supported and well correlated with pronounced antibacterial activity of the tested plant parts.