ABSTRACT
Bupleuri Chinensis Radix, as traditional Chinese medicine, has great use amount in the past dynasties, and the amount of the existing wild resources is not enough to provide market demand. In order to protect the wild resources and expand the source of medicinal materials, the research on the chemical composition, pharmacological action, clinical application and other aspects of Bupleuri Chinensis Radix and other plants in the same genus have been widely carried out. The paper reviews the study of medicinal plant resources of Bupleurum L. in China from the distribution and species, chemical composition of medicinal parts, resources protection and utilization of Bupleurum L. medicinal plants, etc, to provide new clues for Bupleurum L. resources development.
ABSTRACT
Many species of genus Bupleurum L. have been pharmaceutically used mainly in Asia and Europe for thousand years. Their roots are the most popular ingredients in Chinese materia medica prescriptions for the treatment of inflammatory diseases and auto-immune diseases. A plenty of chemical constituents have been isolated and identified from the species in Bupleurum L., such as saikosaponins, polysaccharides, volatile oils, flavonoids, polyacetylenes, lignins, and coumarins, most of which possess a variety of biological activities, especially for the hepatoprotective effect, antitumor activity, immunoregulation, and febrifuge efficacy. Therefore, the species in genus Bupleurum L. could be potential herbs of immunomodulator, antineoplastic, anti-oxidant, etc. Meanwhile, as potential toxicities have been discovered in some constituents, it is urgent to establish a comprehensive quality evaluation system to ensure the safety and efficiency of herbs. This paper reviews on the phytochemical and pharmacological studies, especially for the toxicology and quality control of the species in Bupleurum L., which is a reference for the species in this genus for safe usage and further development. © 2013 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
Object To define the molecular characters of differentiating Bupleurum chinense DC., B. scorzonerifolium Willd. from their allied species B. marginatum Wall. ex DC., B. smithii Wolff var. parvifolium Shan et Y.Li. and B. longiradiatum Turoz. Methods General DNA was isolated from fresh leaves of Bupleurum L. species by CTAB. The random amplified polymorphic DNA (RAPD) was used to identify them. Results Five Bupleurum L. species can be identified by primer OPA-1(5'-CAGGCCCTTC-3'), OPD-8 (5'-GTGTGCCCCA-3'), OPD-11 (5'-AGCGCCATTG-3'). Conclusion The method of RAPD can be used to identify the Bupleurum L. species and its allied species.