ABSTRACT
This experiment was taken to study the changes of cell mediated immunity (CMT) after burn—blast combined injuries in rats and the regulatory effects of indomethacin and PGE_1 on CMT. Mitogen stimulated blastogenic transformation (MSBT) and interleukin—2 (IL—2) were measured in 54 rats before injury and on 1, 3, 5, 7, 14 days after injury. The results showed that MSBT increased significantly on days 3. 5 and 7 but decreased significantly on day 1. IL—2 increased significantly after injury and did not return to normal until day 14. MSBT and IL—2 production were significantly depressed by PGE_1 but on the other hand much enhanced by indomethacin. PGE_1 and indomethacin had no effect on MSBT and IL—2 production in the control group. These results indicate: 1. moderate burn—blast combined injuries can stimulate CMI in rats: 2. PGE_1 can significantly depress IL—2 production and MSBT in rats after injury: 3. Indomethacin can enhance IL—2 production in rats after injury and 4. PGE_1 and indomethacin have no regulatory effect on IL—2 production and MSBT in normal rats.