ABSTRACT
OBJECTIVE: To investigate the effects of Buyanghuanwu decoction on preventing atherosclerosis induced by Hey in rats in vivo. METHODS: The model of atherosclerosis in rats was induced by intragastric gavage of Hey. The intervention group was administered Buyanghuanwu decoction (5, 10, 20 g · kg-1 · d-1). After 8 weeks treatment, the area of atherosclerotic plaques, blood vessel walls and their ratio, and ROS contention in the aorta were analyzed. The positive cell ratio of NF-κB P65 in vascular was detected by immunohistochemistry, and the expression of I-κBα was determined by Western-blotting. RESULTS: The rats in the model group showed evident AS lesions which had significant difference with others groups. Compared with control group, the ROS content of model group was elevated (P < 0.01), the I-κBα protein in the vascular tissue was significantly reduced, and NF-κB was significantly activated. Different doses of Buyanghuanwutang decoction could decrease ROS content (P < 0.01), improve I-κBα protein and inhibit the activation of NF-κB. CONCLUSION: Buyanghuanwutang decoction can prevent atherosclerosis induced by Hcy via inhibiting NF-κB-dependent pathway. Copyright 2012 by the Chinese Pharmaceutical Association.
ABSTRACT
Objective To compare the chemical constituents between the dispensing granules and the decoction of Buyanghuanwutang. Methods TLC and HPLC methods were carried out for comparative analysis. Results TLC indicated the chemical constituents were similar between the dispensing granule and the decoction. The HPLC method was successfully established,and used for taking the contents of paeoniflorin in dispensing granule and the decoction of Buyanghuanwutang. The contents of paeoniflorin in dispensing granule were only one sixth of that in the decoction. Conclusion The chemical constituents of the two preparations are similar,but the contents of paeoniflorin are different.
ABSTRACT
Object To study the antithombotic portion and bioactive fraction of BUYANGHUANWUTANG (BHT), a decoction of astragalus in combination with peony and several other ingredients. Methods The EtOAc portion of BHT extract was repeatedly separated by chromatography and identified by means of spectral analysis. Results One pterocarpan, (6aR, 11aR) 9, 10-dimethoxypterocarpan-3-O-?-D-glucopyanoside (Ⅴ) and four isoflavones, formononetin (Ⅰ), calycosin (Ⅱ), formononetin-7-O-?-D-glucopyanoside (Ⅲ), and calycosin-7-O-?-D-glucopyranoside (Ⅳ) were identified. Conclusion All five compounds were isolated for the first time from BHT.