ABSTRACT
Dipcadi erythraeum Webb & Berthel. is a wild edible species belonging to family Asparagaceae and commonlyused in folk medicine. The D. erythraeum bulbs extract was subjected to chemical investigation using LiquidChromatography-Electrospray Ionization Mass Spectrometry (LC-ESI-MS) technique to identify its polar activeconstituents and evaluated against four human carcinoma cell lines; MCF7, HEPG2, A549, and HCT116. The D.erythraeum bulbs extract revealed 22 phenolic compounds characterized for the first time from the studied species,14 of them were identified as C-glycosyl flavonoids. Moreover, the studied extract showed moderate activity againstMCF7 and HCT116 at 100 µg/ml with cell viability of 43.6% and 48.4%, respectively. From the chemotaxonomicpoint of view, the presence of C-glycosyl flavonoids supported that D. erythraeum has a closer relationship with thespecies of Asparagaceae family than Liliaceae.
ABSTRACT
Objective: An UPLC-LTQ-Orbitrap-MS spectrometry method has been developed for the efficient separation and detection of C-glycosyl flavonoids in the extract of Scutellaria baicalensis differentiation of isomers. Methods: Data were acquired by Orbitrap. MS2, MS3, and MS4 data were triggered by data-dependent acquisition mode. Isomers of C-glycosyl flavonoids were distinguished by a comparison study of mass distribution. Results: A total of 19 compounds were identified as C-glycosyl flavonoids, and several isomers were successfully discriminated. Conclusion: This method is proved to be powerful for the identification of C-glycosyl flavonoids and provides the important complementary information for other flavonoid analysis.