ABSTRACT
Clara cell 10-kD protein (CC10) is one of the most abundant proteins in the respiratory secretions, and plays an important role in immune response, anti-infection and lung injury repair. Chronic obstructive pulmonary disease and bronchial asthma are common chronic lower respiratory tract inflammatory diseases, the pathogenesis of which are intimately related to the abnormal expression of CC10. Therefore, an in-depth research of the biological characteristics of CC10 are helpful for exploring for the important targets for the treatment of chronic respiratory inflammatory diseases in the future. This review summarizes the current research progress in this field.
ABSTRACT
PURPOSE : Human uteroglobin (hUG) is a cytokine-like multifunctional protein that possesses potent immunomodulatory and anti-tumor activity. And, the G38A polymorphism of uteroglobin exon 1 has been associated with the development of immunoglobulin A nephropathy, systemic lupus erythematosus and bronchial asthma. In addition, the 38AA genotype has been related to lower serum levels of uteroglobin than the GG or GA genotypes. Although the hUG gene is one of the candidate tumor suppressors in lung cancer, the uteroglobin gene polymorphism has not been reported upon in lung cancer. Therefore, we studied the frequencies of the G38A polymorphism of the hUG gene in patients with lung cancer and control subjects to investigate its relation with lung cancer. MATERIALS AND METHODS : A matched case control design study was adopted to investigate the possibility of an association between primary lung cancer and the G38A polymorphism. To exclude the possible influence of tobacco smoke exposure, or of age or gender on the development of lung cancer, these factors were matched between the 60 patients and the 60 controls. Genotypes were determined by polymerase chain reaction followed by restriction fragments length polymorphism analysis for the hUG gene. RESULTS : The frequency of the 38A allele in patients was 0.55, which was significantly different from its frequency of 0.37 in controls (p=0.007). Moreover, the frequency of the 38AA genotype was significantly higher in patients (35%) than in controls (15%) (p=0.01). Furthermore, two patients previously diagnosed as having prostate cancer were all genotyped as 38AA. CONCLUSION : The G38A polymorphism of the hUG gene is associated with the development of primary lung cancer in Koreans
Subject(s)
Humans , Alleles , Asthma , Case-Control Studies , Exons , Genotype , Glomerulonephritis, IGA , Lung Neoplasms , Lung , Lupus Erythematosus, Systemic , Polymerase Chain Reaction , Prostatic Neoplasms , Smoke , Nicotiana , UteroglobinABSTRACT
A mammalian expression plasmid pcDNA3.1-hCC10 was constructed and identified, then CC10 protein expression in A549 lung cancer cell line was detected. A 273 bp cDNA fragment was amplified from the total RNA of normal lung tissue by using RT-PCR and cloned into expression plasmid cDNA3.1, and the recombinant plasmid was identified by employing double digestion restriction enzymes HindⅢ and BamH Ⅰ and the cDNA sequence was assayed by the Sanger dideoxymediated chain termination method. The segment was then transfected into the A549 lung cancer cell line. The protein expression of CC10 was detected by immunofluorescence and Western blot.Our results showed that the cDNA fragment included the entire coding region (273 bp). The recombinant eukaryotic cell expression vector of pcDNA3.1-hCC10 was successfully constructed, and the sequence of the insert was identical to the published sequence. A549 cells line transfected with the pcDNA3.1-hCC10 expressed high level of CC10 protein. The recombinant plasmid cDNA3. 1hCC10 may serve as an effective tool for the study of tumorogenesis and tumor treatment.
ABSTRACT
BACKGROUND: Idiopathic interstitial pneumonia is characterized by chronic inflammation and pulmonary fibrosis. The clara cell 10 kD protein (CC10, also designated CC16) is synthesized by the bronchial epithelium and has been suggested to have a potent anti-inflammatory effect. Therefore, CC-10 might be a candidate for controlling the inflammatory events in patients with idiopathic interstitial pneumonia. The aim of this study was to determine if the degrees of pulmonary fibrosis in idiopathic interstitial pneumonia is associated with CC-10 in the BAL fluid. METHODS: The BAL fluid was collected from 29 patients and 10 controls. Densitometric analysis of the western blot assay for the CC-10 was subsequently performed. The RI (relative intensity) of each band was compared according to the diagnosis, the radiological degrees of pulmonary fibrosis and the relative proportion of inflammatory cells in the BAL fluid. RESULTS: There were no differences in the CC-10 expression levels in the BAL fluid between the patients (RI 77.5+/-75.8%) and the controls (70.7+/-39.8%) (p>0.05). In addition, the degrees of pulmonary fibrosis and airway inflammation in patients with usual interstitial pneumonia were not associated with CC-10 expression in the BAL fluid (p>0.05). CONCLUSION: This study suggests that CC-10 expression is not associated with the degrees of pulmonary fibrosis in patients with usual interstitial pneumonia.
ABSTRACT
0.05).The CC10 levels in lung homogenate of the allergic asthma group were significantly decreased than that of control group(P0.05).Conclusion The CC10 levels in lung homogenate is decreased,and has negative correlation with AIPs,which suggests that CC10 may take part in the onset of asthma,and work as an important endogenous anti-inflammatory factor.