ABSTRACT
To investigate the changes of expression location and amount of CUL7 and CCDC8 proteins in the growth plate of normal mice aged 0-4 weeks, and to clarify the roles of CUL7 and CCDC8 proteins in the proliferation and differentiation of tibia growth plate of mice. The expression location and levels of CUL7 and CCDC8 proteins in the tibial growth plate of normal mice aged 0-4 weeks were observed with immunohistochemical staining. CUL7 protein was expressed in the cell cytoplasm and membrane in three zones of tibial growth plate three bands at 0-4 weeks. The expression level and total expression level of CUL7 protein in each zone of growth plate decreased gradually with the increase of week of age( F=369.61, P=0.001). The expression of CUL7 protein decreased most significantly in the proliferative zone, followed by the stationary zone and the hypertrophic zone. CCDC8 protein was mainly expressed in proliferation zone and hypertrophic zone cell membrane and nuclear membrane of growth plate at 0-2 weeks, and mainly expressed in hypertrophic zone cell membrane and nuclear membrane at 3-4 weeks. The expression of CCDC8 protein in the proliferating zone changed inversely with week of age, and the expression of CCDC8 protein in the hypertrophic zone increased over 4 weeks( F=453.67, P<0.001). The total expression of CCDC8 protein in growth plates decreased with the increase of week of age.The expression levels of CUL7 and CCDC8 decreased with the increase of week of age, suggesting that CUL7 and CCDC8 may promote the proliferation and differentiation of growth plate chondrocytes.
ABSTRACT
Objective The aim of this study was to investigate the expression of CCDC8(coiled-coil domain containing 8, P90)and transforming growth factor β 1(TGF- β1)in none small cell lung cancer(NSCLC),and its relationship with clinicopatho-logical characteristics. Methods The expression of CCDC8 and TGF-β1 in NSCLC was analyzed in the TCGA database. The Kap-lan-Meier curve was plotted to analyze its correlation with survival of patient. The expression of CCDC8 and TGF-β1 in 204 cases of conventional paraffin-embedded NSCLC was detected by immunohistochemical staining and their relationship with clinicopatholog-ical features of NSCLC was analyzed. The relationship between the expression of CCDC8 and TGF-β1 and the overall survival(OS) of patients was analyzed by Cox regression. Results The expression of CCDC8 in NSCLC patients was positively correlated with TGF-β1 expression in the TCGA data(P<0. 05). OS was low in patients with the low expression of CCDC8(HR=1. 32,P=0. 0437). In 204 NSCLC tissues,CCDC8 expression was positively correlated with TGF-β1 expression(P=0. 023). Cox univariate analysis of CCDC8 expression in NSCLC was associated with OS(P=0. 013);whereas the expression of CCDC8 and TGF-β1 was not associat-ed with survival in patients with NSCLC(P=0. 967,P=0. 816). Cox multivariate analyses of TNM staging and CCDC8 were progno-sis factors in patients with NSCLC(P=0. 016). Conclusion The expression of CCDC8 is correlated the expression of TGF-β1 in NSCLC. CCDC8 may be a prognostic factor for patients with NSCLC.
ABSTRACT
Objective To determine the relationship between CCDC8 gene and breast cancer.Methods 40 cancerous breast tissue and 22 benign breast tissue were included.qRT-PCR was performed to investigate the expression level of CCDC8 in breast tissue.The correlation between CCDC8 level and the age of patients,tumor size,clinical staging,and the expression levels of estrogen and progesterone receptors,CerbB2,Ki-67,p53 and nm23 were analyzed.Results The expression level of CCDC8 in benign breast tissue (1685±755) was significantly higher than that in cancerous tissues (502.1 ±223.2).Tissues obtained from patients over age 50 showed an increased level of CCDC8 (789.8±367) in comparison to those from patients age 50 or younger (452.5±170.3).The level of CCDC8 expression was negatively correlated with nm23 level (Correlation Coefficient =-0.400,P =0.039),while no correlation was found between CCDC8 and cancer stages,estrogen and progesterone receptor,CerbB2,Ki-67and p53.Conclusion The negative correlations between CCDC8 and age,tumor size and nm23 indicate that CCDC8 is a potential tumor suppressor,influencing the occurrence and progression in breast cancer.