ABSTRACT
Neurolaena lobata (L.) R.Br. ex Cass. (Asteraceae)Is a popular folk remedy for in Central America. The plant is of commercial value in Guatemala but so far there is not any monograph to guide regional laboratories on ensuring identity and chemical tests for this species. As identity test we here run macro and micro morphoanatomical studies of the characters of the vegetative organs. We also developed standard chemical tests for quality by both TLC and HPLC for infusions and tinctures of varying alcoholic strength. Their radical scavenging activities in DPPH and NO were also measured. Macro and micro morphoanatomical characters of the vegetative organs present a set of characteristics to facilitate the identification of dry powdered samples of this species. We developed optimal conditions for the TLC and HPLC phytochemical fingerprints of the 4 most common pharmacopoeial liquid herbal preparations from this herbal drug, namely infusion, 70%, 45% and 20% hydroalcoholic tinctures. Our work provides the Latin-American industry with a set of analyses to establish the identity and chemistry of N. lobata samples for quality control purposes.
Neurolaena lobata (L.) R.Br. ex cass. (Asteraceae) es un remedio popular popular en AmeÌrica Central. La planta tiene un valor comercial en Guatemala, pero hasta el momento no existe una monografiÌa que guiÌe a los laboratorios regionales para garantizar la identidad y las pruebas quiÌmicas para esta especie. Como prueba de identidad proponemos estudios macro y micro morfoanatoÌmicos de los caracteres de los oÌrganos vegetativos. TambieÌn desarrollamos pruebas quiÌmicas de calidad mediante CCF y CLAR para infusiones y tinturas de grado alcohoÌlico variable. TambieÌn se midieron sus actividades de captacioÌn de radicales en DPPH y NO. Los caracteres macro y micro morfoanatoÌmicos de los oÌrganos vegetativos presentan un conjunto de caracteriÌsticas para facilitar la identificacioÌn de muestras de polvo seco de esta especie. Desarrollamos condiciones oÌptimas para las huellas dactilares fitoquiÌmicas de CCF y CLAR de las 4 preparaciones herbales liÌquidas farmacopeÌicas maÌs comunes de esta droga herbal, a saber, infusioÌn, 70%, 45% y 20% tinturas hidroalcohoÌlicas. Nuestro trabajo proporciona a la industria latinoamericana un conjunto de anaÌlisis base para establecer la identidad y la quiÌmica de las muestras de N. lobata con fines de control de calidad.
Subject(s)
Asteraceae/anatomy & histology , Asteraceae/chemistry , Phytochemicals/analysis , Quality Control , Spectrophotometry, Ultraviolet , Free Radical Scavengers , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Asteraceae/ultrastructure , Guatemala , MicroscopyABSTRACT
Pharmaceutical cocrystals are a promising technology that can be used to improve the solubility of poor aqueous compounds. The objective of this study was to systematically investigate the solubility of myricetin (MYR) cocrystals, including their kinetic solubility, thermodynamic solubility, and intrinsic dissolution rate (IDR). The effects of pH, surfactant, ion concentration, and coformers on the cocrystal solubility were evaluated. Furthermore, single crystal structures of MYR, myricetin-isonicotinamide (MYR-INM) and myricetin-caffeine (MYR-CAF) cocrystals were analyzed to discuss the possible reasons for the enhancement of cocrystal solubility from the perspective of the spatial structure. The results indicated that the kinetic solubility of MYR cocrystals was modulated by pH and cocrystal coformer (CCF) ionization in buffer solution, while it primarily depended on the CCF solubility in pure water. In addition, the solubility of MYR cocrystals was increased in a concentration dependent fashion by the surfactant or ion concentration. The thermodynamic solubility of MYR-INM (1:3) cocrystals decreased with the increases of the pH value of the dissolution media. The IDR of MYR cocrystals was faster than that of MYR in the same medium and extremely fast in pH 4.5 buffer. The improved solubility of MYR cocrystals was probably related to the alternate arrangements of MYR and INM/CAF molecules and increased intermolecular distance. The present study provides some references to investigate the solubility behavior of pharmaceutical cocrystals.
ABSTRACT
Infratentorial cerebral hemorrhage due to a direct carotid–cavernous fistula (CCF) is very rare. To our knowledge, only four such cases have been reported. Cerebellar hemorrhage due to a direct CCF has not been reported. We describe a 63-year-old female who presented with reduced consciousness 3 days after undergoing a maxillectomy for maxillary cancer. Computed tomography showed a cerebellar hemorrhage. Magnetic resonance angiography showed a left-sided direct CCF draining into the left petrosal and cerebellar veins through the left superior petrosal sinus (SPS). Her previous surgery had sacrificed the pterygoid plexus and facial vein. Increased blood flow and reduced drainage could have led to increased venous pressure in infratentorial veins, including the petrosal and cerebellar veins. The cavernous sinus has several drainage routes, but the SPS is one of the most important routes for infratentorial venous drainage. Stenosis or absence of the posterior segment of the SPS can also result in increased pressure in the cerebellar and pontine veins. We emphasize that a direct CCF with cortical venous reflux should be precisely evaluated to determine the hemodynamic status and venous drainage from the cavernous sinus.
Subject(s)
Female , Humans , Middle Aged , Cavernous Sinus , Cerebral Hemorrhage , Consciousness , Constriction, Pathologic , Drainage , Fistula , Hemodynamics , Hemorrhage , Magnetic Resonance Angiography , Veins , Venous PressureABSTRACT
Se caracterizaron los extractos etanólicos de hojas y cortezas de 13 especies de la familia Lauraceae mediante cromatografía en capa fina de dos dimensiones (2D-CCD). Los datos posteriores se analizaron mediante técnicas de análisis estadístico multivariado (cluster y análisis de componentes principales (PCA)). Lo anterior permitió hacer una distinción entre los extractos obtenidos de diferentes partes de la planta (hojas y cortezas). Se observó, además, que la metodología usada es capaz de diferenciar entre extractos obtenidos a partir de especies de Lauraceae y los de otras familias de plantas.
Leaves and barks ethanolic extracts from 13 Lauraceae species were characterized through two-dimensional thin layer chromatography (2D-TLC). The subsequent data was analized through multivariate statistical analysis techniques (cluster analysis and principal components analysis (PCA)). This allowed to do a distinction between extracts obtained from different parts of the plant (leaves and bark). In addition, it was observed that the implemented methodology is able to differentiate between extracts obtained from Lauraceae species and some obtained from other plant families.
Caracterizaram-se os extratos etanólicos de folhas e casca obtidos a partir de espécies da família Lauraceae por cromatografia em camada fina em duas dimensões (2D-CCF). Os dados obtidos foram analisados utilizando técnicas de análise estatística multivariada tipo análise de cluster e análise de componentes principais (PCA). As técnicas estadísticas permitiram fazer uma distinção entre os extratos obtidos a partir de diferentes partes da planta (folhas e casca). Além disso, observou-se que o método utilização é capaz de diferenciar entre os extratos provenientes de espécies de Lauraceae daqueles obtidos a partir de outras famílias de plantas.
ABSTRACT
ADPKD presenting as congestive cardiac failure due to dilated cardiomyopathy is rare. ADPKD is an inherited systemic disorder with major renal manifestation and in some cases extrarenal manifestation or combination of both. In this report 45 year male patient presented with complains of dyspnoea, abdominal distension, pain right hypochondriac region. He was hospitalized, examined clinically and advised various bio-chemical and imaging tests. The finding was suggestive of ADPKD with dilated cardiomyopathy with congestive cardiac failure. He was managed with diuretics, ACE inhibitors, digoxin, Moist oxygen inhalation and he responded to the treatment.
ABSTRACT
Conventional endovascular treatment for carotid cavernous fistula (CCF) involves a direct delivery of either coils, detachable balloon or both to the fistula with end point of CCF resolution and carotid artery preservation. But in few cases with severe laceration of carotid artery, the feasible endovascular technique applicable is by blocking the filling of fistula from cerebral circulation. This method known as trapping technique which implicates carotid artery occlusion, was performed in our present case with good result.
ABSTRACT
Dural carotid-cavernous fistula (CCF) is characterized by arteriovenous communications between the meningeal branches of the internal or external carotid arteries and the cavernous sinus. Although the triad of chemosis, proptosis, and ocular bruit has been regarded as a classic sign of CCF, dural CCF often lacks these features. Dural CCF is a rare cause of ophthalmoplegia, and so it may be overlooked when the classic symptoms are absent. We report herein a case of bilateral dural CCF that presented as unilateral isolated abducens nerve palsy.
Subject(s)
Abducens Nerve , Abducens Nerve Diseases , Carotid Artery, External , Cavernous Sinus , Caves , Diplopia , Exophthalmos , Fistula , OphthalmoplegiaABSTRACT
The species Alpinia purpurata is scarcely cited as to ethnopharmacology and phytochemistry. This study aimed to analyze bioactive compounds through high-performance liquid chromatography (HPLC). Hydroalcoholic crude extract was obtained from A. purpurata dried leaves. Folin-Ciocalteau method was used to quantify total phenols, using gallic acid as standard. The obtained result was 15.6 mg GAE g-1. The crude extract was partitioned with the solvents ethyl acetate and butanol, followed by thin-layer chromatography (TLC) and HPLC. The flavonoids kaempferol-3-O-glucuronide and rutin were detected at a higher concentration in ethyl acetate and butanolic extracts. The butanolic extract contains the highest flavonoid percentage (94.3 percent). A. purpurata presents important flavonoids of therapeutic use, already verified for A. zerumbet. This is the first study verifying the presence of flavonoids in A. purpurata extracts.
A espécie Alpinia purpurata apresenta poucas citações referentes a etnofarmacologia e fitoquímica. Este estudo propõe a análise de substâncias bioativas através da técnica de cromatografia líquida de alta eficiência (CLAE). O extrato bruto hidroalcóolico foi obtido a partir de folhas secas de A. purpurata. A quantificação de fenóis totais foi realizada pelo método de Folin-Ciocalteau, usando ácido gálico como padrão. Como resultado, foi verificado 15,6 mg EAG g-1. O extrato bruto foi particionado com os solventes acetato de etila e butanol e depois analisado por cromatografia em camada delgada e CLAE. Nos extratos acetato de etila e butanólico foi detectada a presença dos flavonóides kaempferol-3-O-glicuronídeo e rutina, em maior concentração. O extrato butanólico contém a maior porcentagem de flavonóides (94,3 por cento). Esta espécie possui flavonóides importantes no uso terapêutico, já antes verificados para a espécie A. zerumbet. Este é o primeiro trabalho que verifica a presença de flavonóides em extratos de A. purpurata.
Subject(s)
Flavonoids/analysis , Chromatography, High Pressure Liquid/methods , Alpinia/physiology , Rutin/analysis , /analysis , Zingiberaceae , Polyphenols/pharmacokineticsABSTRACT
Objective To explore the feasibility and value of special carotid cavemous fistula(CCF). Metheds In 28 cases with traumatic history, cerebral angiographies were performed via percutaneous transfemoral route before endovascular embolization. Results Bilateral CCF in 6 cases,unilateral CCFs including mutileakage in 2 cases and small fistulae in 4 cases,CCF combined with fracture segment in 11 cases,traumatic aneurysms in 3 cases,NPPB after embolizationin 2 cases. The fistulas of 9 cases were occluded with patency of internal carotid artery(ICA),and ICA were occluded in 19cases.Conclusions Endovascular embolization of carotid cavernous fistula was safe and effective.This methed should be the first choice for the treatment.Different techniques of endovascular embolization should be undertaken for different CCF.
ABSTRACT
Objective To evaluate the application value of detachable balloon in today's interventional neuroradiology. Methods 38 cases with vascular lesion in the area of cranial cervical region ( 26 cases with CCF, 3 with giant aneurysm in the cavernous segment of internal carotid artery, one with fusiform aneurysm in internal carotid artery, and 8 with AVF in the area of cranial cervical) were treated by detachable balloon for embolizing the shunt or scrificing the lesion ipsilateral internal carotid artery. Results All 38 cases were cured without complication and sequeulae. Of them, anatomical cure was aquired in 21 cases, lesion cure in 17 cases. Conclusions Detachable balloon is still the first choice of embolization material in today's endovascular therapy of some vascular lesions in the area of cranial cervical region.
ABSTRACT
Traumatic carotid-cavernous fistula(CCF) is an uncommon situation resulting from trauma. The blood shunted from the internal carotid artery to the cavernous sinus results in pulsation exophthalmos,orbital headache, bruit, loss of visual acuity, diplopia, ophthalmoplegia. We were recently faced with such a case. The patient sustained fracture of the mandible during sports activity. Five months later, all the classic signs and symptoms of CCF had appeared. A carotid angiography confirmed the presence of a CCF. The management was achieved intravascularly by platinum electrothrombosis embolization. The main signs and symptoms of the CCF had resolved, but the patient remained in a visual loss of affected eye.
Subject(s)
Humans , Angiography , Carotid Artery, Internal , Cavernous Sinus , Diplopia , Fistula , Headache , Mandible , Mandibular Fractures , Ophthalmoplegia , Platinum , Sports , Visual AcuityABSTRACT
We analyzed 20 cases of traumatic carotid cavernous fistula(CCF) during the recent 10 years The results are summarized as follows: 1) In 18 cases(90%), the clinical symptoms & signs of CCF occurred within 2 months after trauma. 2) The sites of fistulae were common in horizontal segment(40%) and at the junction(30%) between horizontal segment and posterior ascending segment of cavernous portion of internal carotid artery. 3) The main draining veins of CCF were the superior ophthalmic vein(90%) and the inferior petrosal sinus(70%). 4) The methods of treatment were occlusion of fistula with balloon(9 cases), occlusion of cavernous ICA with balloon(2 cases), ligation of cervical ICA with Poppen's clamp(4 cases) and trapping(2 cases). Two patients were not treated and another patient was healed spontaneously. 5) The frequency and severity of complication was significantly decreased in cases treated by detachable balloon occlusion than by direct cervical ICA ligation or trapping procedures. 6) The procedure using the self-sealed goldvalve balloon was simple, but had a risk of premature separation and premature deflation.
Subject(s)
Humans , Balloon Occlusion , Carotid Artery, Internal , Fistula , Ligation , VeinsABSTRACT
Many different type of embolic material, such as muscles Gelfoam, porcelain, and detachable balloon, etc, has been used for the treatment of carotid-cavernous fistula. In an effort to achieve better results the authors used laminaria, a sea-weeds root, as a newly-tried embolus which has a unique characteristic of expanding gradually within 4 hours 3-4 times from its original width, but not in length, when in contact with any type of fluid. A very special feature of the laminaria is that the initial hard and coarserness is slowly transformed while expanding into a rounded softness as to allow no menhanical injury to the surrounding vessels of tissue. The laminaria was formed into a reversed bowling-pin shape measuring 8x1.5mm with tapered tail and a round head in which contains a sliver clip as a radiographic marker. An extension of standard angiographic technique ahs been used to complete an alternative method of treatment. This technique involves introducing the cather harboring the laminaria by the percutaneous route either transfemoral or directly transcarotid and releasing the terminal internal carotid artery at the site of the fistula. Releasing a few of the shaped laminaria piece from the catheter tip has resulted in a successful obliteration of the fistula in 7 cases out of total 8 of carotid-cavernous fistula.