ABSTRACT
Objective To explore the proportion of CCR7loPD-1hi follicular helper T cell (Tfh) in peripheral blood of the patients with systemic lupus erythematosus (SLE) and its clinical role. Methods Peripheral blood samples were collected from 31 SLE patients, 29 rheumatoid arthritis (RA) patients, 12 Sjögren’s syndrome (SS) patients and 37 healthy controls. Flow cytometry was used to detect the expression levels of C-X-C chemokine receptor 3 (CXCR3), inducible costimulator (ICOS) and signaling lymphocytic activation molecule family member 5 (SLAMF5) on surface of Tfh, and the frequencies of CCR7loPD-1hi Tfh and CCR7hiPD-1lo Tfh in peripheral blood. The correlation between the proportion of CCR7loPD-1hi Tfh in peripheral blood of SLE patients and clinical indicators and the proportion of plasmablasts was analyzed. Results The expression levels of CXCR3, ICOS and SLAMF5 were significantly higher on the surface of the CCR7loPD-1hi Tfh compared with those of the CCR7hiPD-1lo Tfh (t=3.73, 5.06 and 8.27; all P0.05). The proportion of CCR7loPD-1hi Tfh in peripheral blood of the SLE patients was positively correlated with systemic lupus erythematosus disease activity index (SLEDAI), serum anti-double-stranded DNA (dsDNA) titers and the proportion of plasmablasts (r=0.447 1, 0.517 4 and 0.466 9; all P<0.05). Conclusion Increased proportion of CCR7loPD-1hi Tfh in peripheral blood of the SLE patients is associated with increased SLEDAI and increased proportion of plasmablasts; and detecting the Tfh subsets can indirectly reflect the functional status of germinal center and B lymphocytes, which is of great significance for diagnosis, monitoring and prognosis of SLE.
ABSTRACT
To observe the expression of CCL19 in colorectal carcinoma tissues and to investigate its effect on proliferation, migration and invasion of colorectal cancer cells. Methods: The expression of CCL19 in 85 confirmed colorectal carcinoma tissues and the corresponding adjacent normal tissues was detected by quantitative real-time PCR (qRT-PCR) and immunohistochemistry of tissue microarray. SW620 cell line highly expressing CCR7(CCL19 receptor) as screened by qRT-PCR and Western blotting analysis was stimulated by different concentrations of rh-CCL19 (0, 10, and 100 ng/mL). Then the cell proliferation, migration and invasion capacity were examined by CCK-8, wound healing assay, and Transwell assay, respectively. Results: Immunohistochemistry and qRT-PCR results demonstrated that CCL19 expression was significantly lower in the colorectal carcinoma tissues than in the normal tissues (P<0.05). CCL19 expression was associated with tumor size and invasion depth (P<0.01). Treatment with rh-CCL19 significantly decreased the proliferation, migration and invasion capacity of SW620 cells(P<0.05). Conclusion: CCL19 expression in colorectal cancer tissues is lower than that in the adjacent normal tissues, and the expression is associated with tumor size and invasion depth. CCL19 can inhibit the proliferation, migration and invasion capacity of SW620 cells.