Angiosarcoma de cuero cabelludo. revisión de la literatura actual a propósito de un caso / Angiosarcoma of the scalp. a case report and review of the literature

RESUMEN El angiosarcoma es un tumor de células neoplásicas de origen vascular, cuya presentación clínica es variada y su diagnóstico es tardío. Se presenta el caso de una paciente de 83 años, con múltiples úlceras dolorosas en cuero cabelludo, con diagnóstico incierto, por lo que se realiza biopsiaincisional de la lesión, detectándose un angiosarcoma. La inmunohistoquímica de la lesión fue positiva para CD34 y vimentina, negativo para S-100 y Melan-A. Este tumor se presenta en adultos mayores, siendo altamente invasivo y de mal pronóstico. Su diagnóstico requiere un alto índice de sospecha y a la fecha, no existe un tratamiento definido para esta patología.

SUMMARY Angiosarcoma is an aggressive malignant tumor of the vascular endothelial cells, and its clinical presentation is varied and of late diagnosis. The present case has a female patient, aged 83 who has multiple painful ulcers in scalp, whose lack of treatment response required a biopsy, where it was found an angiosarcoma. The immunohistochemistry of the lesion was positive for CD34 and vimentin, and negative for S-100 and Melan-A. This tumor develops in older people and is highly invasive withpoor prognosis. Its diagnosis requires high rates of suspicion and todate there is not a defined treatment for this pathology.

CD34+ bone marrow stem cells homing to embolized rabbit lung tissue / 第二军医大学学报

Objective To observe the homing of bone marrow stem cells (BMSCs) to the embolized lung tissues after mobilization and to investigate the potential mechanisms. Methods Thirty healthy Chinese big-ear rabbits were randomized into two groups regardless of gender: pulmonary thromboembolism (PTE) group (model group), PTE+granulocyte colony-stimulating factor (G-CSF) mobilization group (experimental group), with each group having 15 animals. Stable PTE models were established by injecting autologous thrombus into the femoral veins of the animals (model group). The animals in the experimental group received daily hypodermic injection of G-CSF (10 μg/[kg��d]) for five days, which was started 4 days before the establishing PTE models and ended at one day after the models were established. The animals were sacrificed 24 hours after modeling in both groups for general sample observation. Hematoxylin-eosin (HE) staining was done for the samples. Immunohistochemical analysis was done to detect the expressions of CD34 and SDF-1 in the embolized area, edge area, and normal area. A medical image processing software was used to analyze the results of immunohistochemical results to calculate the relative contents of CD34 and SDF-1. Results (1)General sample observation: the animals exhibited the damage of lung tissues in both groups, including splinter hemorrhage sites, local pale region and atelectasis. (2)Microscopic pathological observation: the embolized areas of all animals exhibited interstitial edema and hyperemia, and many red blood cells entered the pulmonary alveoli. Increased monocyte infiltration was detected in the experimental group. (3)Results of immunohistochemical analyses and image analyses of CD34: Most CD34 expression was found in the embolized area, very weak expression was found in the edge area, and hardly any was detected in the normal area. CD34 expression in embolized area was significantly higher in the experimental group than that in the model group (P<0.01). More monocytes expressing CD34 were seen in the experimental group and only a small number of them were found in the model group. (4) Results of immunohistochemical analyses and image analyses of SDF-1: Most SDF-1 expression was found in the embolized area, and no expression was found in the edge area and normal area. SDF-1 expression in embolized area was significantly higher in experimental group than in the model group (P<0.01). Conclusion BMSCs expressing CD34 can home to the embolized lung tissues after PTE. The increased SDF-1 expression may be one of the mechanisms of BMSCs homing to embolized lung tissues after PTE. Mobilization with G-CSF can increase SDF-1 expression in embolized area, which can attract more BMSCs to home to embolized lung tissues.

Expressão imunohistoquímica do marcador tumoral CD34 e P27 como fator prognóstico em adenocarcinoma de próstata clinicamente localizado após prostatectomia radical / Immunohistochemistry expression of tumor markers CD34 and P27 as a prognostic factor of clinically localized prostate adenocarcinoma after radical prostatectomy

OBJETIVO: Analisar a expressão imunoistoquímica do marcador CD34 e p27, como fator prognóstico em pacientes com neoplasia de próstata localizada. MÉTODOS: Análise de 100 casos de pacientes portadores de neoplasia prostática localizada submetida à cirurgia curativa. Realizou-se o preparo histológico habitual, seguido da reação imunoistoquímica para a detecção do acúmulo da proteína CD34 e p27 seguida de análise estatística. RESULTADOS: Na avaliação do marcador P27 e na correlação com as variáveis, observou-se diferença significativa no escore de Gleason com expressão positiva (P27 positivo) relacionada com PSA médio mais baixo (p=0,091), escore de Gleason mais baixo (p<0,0001) e menor área de tumor no CD34 (p=0,036). Correlacionando-se o marcador CD34 na área tumoral observou-se quanto menor o CD34 positivo menor é o valor do PSA (p<0,0001), e menor é o escore de Gleason (r=0,5726 ; p<0,0001) e quanto maior o CD34 positivo maior é o estadiamento (r=0,3305 ; p<0,0001) e a chance de recidiva (p=0,002). Os pacientes com estadiamento mais alto, também tinham maior área CD34 positivo (p<0,0001). CONCLUSÃO: Os marcadores P27 e CD34 estão associados com os eventos próprios ao câncer de próstata; contudo, apenas o CD34 foi capaz de determinar a possibilidade de recidiva bioquímica.

OBJECTIVE: to analyze the immunohistochemical expression of P27 and CD34 markers as prognostic factors in patients with localized prostate cancer. METHODS: analysis of 100 patients with localized prostate cancer submitted to curative surgery. We carried out the usual histological preparation, followed by immunohistochemistry to detect the accumulation of P27 and CD34 protein followed by statistical analysis. RESULTS: in the evaluation of P27 marker and on the correlation with the variables we found significant difference in Gleason score with positive expression (positive P27) related to lower mean PSA (p = 0.091), lower Gleason score (p < 0.0001) and smaller tumor area in CD34 (p = 0.036). Regarding the CD34 marker at the tumor area, it was observed that the smaller the positive CD34, the lower the PSA value (p < 0.0001) and lower the Gleason score (r = 0.5726, p < 0.0001), and the higher the positive CD34, the higher the staging (r = 0.3305, p <0.0001) and the chance of recurrence (p = 0.002). Patients with higher stage also displayed larger positive CD34 areas (p < 0.0001). CONCLUSION: the markers CD34 and P27 are associated with events specific to prostate cancer, however, only CD34 was able to determine the possibility of biochemical recurrence.

Effect of radiotherapy on neointima of prosthetic vascular graft after prosthetic vessel replacement of abdominal aorta / 中国普通外科杂志

0.05) between the radiotherapy and control groups,and the coverage of vascular endothelial cell was incomplete in each group;8 weeks after surgery,the intimal thickness of radiotherapy group was statistically thinner than that of control group(P