ABSTRACT
[Objective] To study the mechanism of Ruangan Granule (RG) in counteracting the experimental liver fibrosis and cirrhosis in SD rats. [Methods] As compared with colchicines, effect of RG in small and large dosages on serum transforming growth factor ? (TGF ?1 ), hydroxyproline (Hyp), collagen Ⅰ and Ⅲ , hepatic function and hepatic pathology were observed in rats. [ Results ] Both RG and colchicines could decrease serum levels of alanine aminotransferase (ALT) and alkaline phosphatase (AKP) and increase serum levels of total protein and albumin in rats with liver fibrosis induced by bovine serum albumin (P 0.05). Effect of large-dosage RG in improving hepatic pathology was better than that of colchicines. [Conclusion] RG has an inhibitory effect on the formation of liver fibrosis and cirrhosis. Its mechanism may be related to the improvement of hepatic function, decrease of serum TGF ft , collagen Ⅰ and Ⅲ and Hyp levels.
ABSTRACT
[Objective] To investigate the effect of Ruangan Kangxian Prescription (RKP), a prescription mainly composed of Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Catechu, Radix Stephaniae Tetrandrae and Polyporus, for rats liver fibrosis induced by carbon tetrachloride (CC14) and to explore its mechanism. [Methods] Rats with liver fibrosis were induced by CC14 and were randomized to RKP group, normal group, Biejia Ruangan Tablet (BRT) group and model group. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN) , procollagen Ⅲ (PCⅢ ) were detected; hepatic histopathology and immunohistochemical result of transforming growth factor ?1 (TGF ?1 ) were also examined. [ Results ] Death rate of rats was reduced; serological parameters of hepatic function and liver fibrosis were improved and liver fibrosis showed by immunohistochemical method, HE staining and Masson staining was inhibited in RKP and BRT groups (P 0.05). [ Conclusion ] RKP and BRT have the similar effect in preventing CC14-induced liver fibrosis in rats and its mechanism may be related to the counteraction of expression of TGF ?1 .