ABSTRACT
Objective To study the effect of curcumin on the expression of inflammatory factors in model rats with chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS) type Ⅲ.Methods The rats were randomly divided into following groups: sham-operated group(sham), CP/CPPS model group(model), curcumin 50 mg group(cur-50 mg) and curcumin 100 mg group(cur-100 mg) and p38 inhibitor group(SB203580).Drug was given to each treatment group for 12 days by intraperitoneal injection.The levels of TNF-α, p38, COX-2 mRNA was measured by Real-Time PCR.The expressions of TNF-α and COX-2 proteins were measured by immunohistochemistry.The levels of p38, p-p38 and NF-κB proteins were detected by Western blot.Results NF-κB, p-p38, TNF-α, COX-2 proteins and TNF-α, p38, COX-2 mRNA in model group were higher than those in the sham group (P<0.01);cur-100 mg group and SB203580 group could significantly relieve these change in the model group (P<0.01);COX-2 mRNA and protein in cur-100 mg group decreased more than that in SB203580 group;p-p38 was positively correlated with NF-κB in curcumin groups and SB203580 group compared with model group (P<0.01).Conclusions Curcumin can reduce the expression of inflammatory cytokines NF-κB, TNF-α, COX-2 and p-p38 in rats with CP/CPPS type Ⅲ.
ABSTRACT
<p><b>OBJECTIVE</b>To compare the clinical efficacy differences among acupuncture combined with western medicine, acupuncture alone and western medicine alone for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).</p><p><b>METHODS</b>Ninety patients were randomly assigned into a needle-medicine group, an acupuncture group and a western medicine group, 30 patients in each group. The patients in the needle-medicine group were treated with acupuncture combined with western medicine; the scalp points included Shenting (GV 24), Xinhui (GV 22), Qianding (GV 21), Baihui (GV 20), Chengguang (BL 6), Tongtian (BL 7), etc. The body points were Zhongji (CV 3), Guanyuan (CV 4), Pangguangshu (BL 28), Ciliao (BL 32), etc. The acupuncture was given 30 min per treatment, once a day. Besides, oral administration of 0.2g levofloxacin (twice per day) and 0.2 mg tamsulosin (once a day) was applied. The patients in the acupuncture group and western medicine group were treated by acupuncture and western medicine respectively. 12-d treatment was taken as one session, and totally 2 sessions were given. The clinical efficacy of the three groups after treatment was compared as well as the National Institutes of Health-Chronic Prostatitis Symptom Index (NIH-CPSI) total score and pain score, self-rating anxiety scale (SAS) and self-rating depression scale (SDS) before and after treatment.</p><p><b>RESULTS</b>During the trial two patients dropped out, as a result, 30 patients in the needle-medicine group, 29 patients in the acupuncture group and 29 patients in the western medicine group were included in the analysis. After treatment, 21 patients were cured, 6 patients were markedly effective, 2 patients were effective and 1 patient failed in the needle-medicine group;12 patients were cured, 10 patients were markedly effective, 5 patients were effective and 2 patients failed in the acupuncture group; 11 patients were cured, 12 patients were markedly effective, 4 patients were effective and 2 patients failed in the medicine group; the efficacy in the needle-medicine group was superior to those in the acupuncture group and medicine group (both<0.05). Each score was improved after treatment in each group (all<0.01); the total score of NIH-CPSI as well as SAS and SDS scores in the needle-medicine group were superior to those in the acupuncture group and medicine group (<0.05,<0.01); the pain scores of NIH-CPSI in needle-medicine group and acupuncture group were superior to that in the medicine group (<0.05,<0.01), but the difference between the needle-medicine group and acupuncture group was not significant (>0.05).</p><p><b>CONCLUSIONS</b>The efficacy of acupuncture combined with western medicine for CP/CPPS is superior to that of acupuncture alone and western medicine alone, which could improve the symptom of prostatitis as well as status of anxiety and depression.</p>