ABSTRACT
Cathepsin D (CTSD), the major lysosomal aspartic protease that is widely expressed in different tissues, potentially regulates the biological behaviors of various cells. Follicular granulosa cells are responsive to the increase of ovulation number, hence indirectly influencing litter size. However, the mechanism underlying the effect of CTSD on the behaviors of goat granulosa cells has not been fully elucidated. This study used immunohistochemistry to analyze CTSD localization in goat ovarian tissues. Moreover, western blotting was applied to examine the differential expression of CTSD in the ovarian tissues of monotocous and polytocous goats. Subsequently, the effects of CTSD knockdown on cell proliferation, apoptosis, cell cycle, and the expression of candidate genes of the prolific traits, including bone morphogenetic protein receptor IB (
ABSTRACT
Purpose To detect the expression of Cathepsin D(CTSD),heat shock protein 90α (HSP90α) and epidermal growth factor receptor (EGFR) in gastric cancer (GC) tissues and to analyze the association of their expression levels and clinical pathological features of GC,the role of CTSD,HSP90α,EGFR in the carcinogenesis,progression,invasion and metastasis of GC.Methods The expression of CTSD,HSP90α and EGFR in normal gastric mucosa,dysplasia adjacent to carcinoma,GC tissues and lymph node metastasis was measured by immunohistochemistry (IHC) and in situ hybridization (ISH).Results In 110 cases of normal gastric mucosa,83 cases of dysplasia adjacent to carcinoma,110 cases of GC and 78 cases of the lymph node metastasis,the positive rate of CTSD,HSP90α,EGFR protein was 0,18.7％,80.91％ and 92.31％,0.9％,15.66％,75.45％ and 89.74％,0,12.05％,69.09％ and 84.62％,respectively.Correlation analysis showed that the expression CTSD,HSP90α and EGFR in GC tissues was positively correlated (rs =0.853,P ＜ 0.05,rs =0.639,P＜0.05,rs =0.734,P＜0.05).Expression of CTSD,HSP90α and EGFR in GC was correlated with the degree of tumor differentiation,invasive depth,number of lymph node metastasis and TNM stages (P ＜ 0.05).There was no significant correlation with the patient's age,sex,tumor size (P ＞0.05).Conclusion CTSD,HSP90α and EGFR may be associated with malignant behavior,development and invasion and metastasis of GC.