ABSTRACT
El Tricoepitelioma Múltiple Familiar (TMF) constituye una rara enfermedad autosómica dominante, se caracteriza por la aparición de múltiples pápulas color piel, monomorfas, simétricas, ubicadas en la región central de la cara. El diagnóstico es histopatológico, donde se encuentran tricoepiteliomas, los cuales son neoplasias anexiales benignas que se originan en los folículos pilosos. La condición es de comportamiento indolente, pero con una importante repercusión estética y de difícil manejo. Al ser esta una entidad poco frecuente, el objetivo de este artículo es actualizar los aspectos más relevantes de esta enfermedad. Se presenta el caso de una paciente de 23 años con lesiones faciales típicas en quien se confirmó el diagnostico de TMF
Familial Multiple Trichoepithelioma (FMT) is a rare autosomal dominant disease, characte-rized by the appearance of multiple papules of skin color, monomorphic, symmetrical and located in the central region of the face. The diagnosis is based on histopathological features of trichoepitheliomas, which are benign adnexal neoplasms that originate in the hair follicles. The condition has an indolent behavior but it has an important aesthetic repercussion and it's difficult to treat. As this is a rare entity, the objective of this article is to update the most relevant aspects of this disease. We present the case of a 23 year old patient with typical facial lesions in whom the diagnosis of FMT was confirmed.
Subject(s)
Humans , Female , Young Adult , Skin Neoplasms/genetics , Skin Neoplasms/pathology , Neoplastic Syndromes, Hereditary/genetics , Neoplastic Syndromes, Hereditary/pathology , Facial Neoplasms/genetics , Facial Neoplasms/pathologyABSTRACT
Objective:To study the expression of tumor suppressor factors SEMA3F,CYLD and miR-454 in rectal cancer.Methods:21 patients with colorectal cancer admitted in our hospital from January to March in 2016 were selected as the study materials.The tumor inhibitoryfactor SEMA3F in rectal cancer tissue,cancerous surrounding tissue and normal rectum tissue were detected respectively,CYLD and miR-454,and to analyze the correlation between tumor suppressor factors SEMA3F,CYLD and miR-454,and to explore their roles in the carcinogenesis and progression of rectal cancer.Results:The expression of SEMA3F,CYLD and miR-454 in cancer tissue,paracancer tissue and normal tissue were statistically significant (P＜0.01).The levels of SEMA3F and CYLD were significantly lower in patients with metastasis than those without metastasis,and miR-454 was significantly higher than that in patients without metastasis (P＜0.01).The expression level of SEMA3F and CYLD in CRC tissues was significantly higher than that in moderately and poorly differentiated tissues,and the expression of miR-454 was significantly lower than that in moderately and poorly differentiated ones (P＜0.01).There was significant negative correlation between tumor suppressor CYLD and miR-454 (r=-0.971,P＜0.01).There was a significant negative correlation between tumor inhibitory factor SEMA3F and miR-454(r=-0.955,P＜0.01).Conclusion:The tumor suppressor factors SEMA3F and CYLD play an important role in inhibiting the formation and proliferation of cancer cells in colorectal cancer.The expression of tumor suppressor factors SEMA3F and CYLD in colorectal cancer is high in the early stage and gradually declines with the progression of the disease.MiR-454 can promote the growth of rectal cancer cells The expression of tumor suppressor factors SEMA3F,CYLD and miR-454 in colorectal carcinoma were significantly higher than those in SEMA3F and CYLD.The expression of tumor suppressor factors such as SEMA3F,CYLD and miR-454 were significantly correlated with the progression and prognosis of rectal cancer The evaluation has important reference value.
ABSTRACT
Objective To investigate the effect of miR-181b on the proliferation and apoptosis of thyroid papillary carcinoma cells by down-regulating CYLD protein and its mechanism. Methods qPCR was used to detect the expression and difference of miR-181b in papilla-ry thyroid carcinoma and normal thyroid tissue. Western blotting was used to detect the regulation between miR-181b and CYLD protein. Clone formation assay was used to detect the proliferation of thyroid cancer cells after inhibition of miR-181b. Flow cytometry was used to detect the apoptosis of thyroid carcinoma cells after inhibition of miR-181b. Results Compared with normal thyroid tissue,the expression of miR-181b in papillary thyroid carcinoma was up-regulated,the difference was statistically significant(P<0. 05). The expression level of miR-181b in FTC-133 cell line was relatively high. Western blotting confirmed that miR-181b could directly regulate the expression of CYLD protein. Inhi-bition of miR-181b expression can inhibit the proliferation of thyroid cancer cells,and to some extent promote its apoptosis behavior. Conclu-sion miR-181b can regulate the expression of CLYD and affect the proliferation and apoptosis of thyroid cancer cells.
ABSTRACT
Tumor suppressor CYLD is a deubiquitination enzyme that could regulate NF-?B and JNK signal pathways by deubiquitinating TRAFs,NEMO,Bcl-3 and p53,and in turn regulating the cell cycle and apoptosis.Loss or deficiency of CYLD would lead to the development of skin tumor,including multiple familial trichoepithelioma(MFT),familial cylindromatosis(FC),Brooke-Spiegler syndrome(BSS).Cancers like uterine cervix,kidney,and colon and hepatocellular carcinoma are related to the down-regulation of CYLD.