Chemical constituents from fruits of Tribulus terrester / 中草药

Objective: To study the chemical constituents from the fruits of Tribulus terrester. Methods: The constituents were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, preparative HPLC and TLC methods. The structures were identified by HR-ESI-MS and spectral analysis methods. Results: Fourteen compounds were isolated from the fruits of Tribulus terrester and the structures were identified as N-p-feruloyltyramine (1), N-trans-p-coumaroyl-3-O- methyldopamine (2), methyl ferulic acid (3), caffeic acid methyl ester (4), protocatechuic acid methyl ester (5), trifilines A (6), 5β-spirost-25(27)-en-3β-ol-12-one 3-O-{β-D-glucopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranoside} (7), tribulosaponin B (8), isoterrestrosin B (9), 25(R)-spirostane-3,5-diene-12-one (10), 25 (R)-spirostane-24β-ol-4-ene-3,12-dione (11), 26-O-β-D-glucopyranosyl-(25S)-5α-furostane-20 (22)-en-12-one-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl- (1→4)]-β-D-galactopyranoside (12), terrestrinone A1 (13), and terrestrinone A2 (14). Conclusion: Compounds 1-7 are obtained from the Zygophllyaceae for the first time.

Determination of six polyphenols in different parts of Queshe tea extracted by different processes by HPLC / 中草药

Objective: To establish HPLC method for the determination of 6'-O-caffeoylarbutin (1), arbutin (2), robustaside A (3), p-hydroxybenzoic acid (4), caffeic acid (5) and caffeic acid methyl ester (6) of Queshe tea. Methods: Using the SunFire® C18 (250 mm×4.6 mm, 5 μm) chromatographic column, mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution, the flow rate at 1.0 mL/min, and column temperature at 30 ℃ to optimum the extraction method of Queshe tea and investigate the linearity, stability and repeatability of the method. Results: With distilled water as the extraction solvent, the highest extraction rate can be obtained by ultrasonic extraction for 20 min. Under the above chromatographic conditions, 6'-O-caffeoylarbutin (1), arbutin (2), robustaside A (3), p-hydroxybenzoic acid (4), caffeic acid (5) and caffeic acid methyl ester (6) have good separation effect, and the experiment has good linearity, stability and repeatability. Conclusion: The method is simple, sensitive and accurate, and can be used for the detection of the above six main chemical components.