ABSTRACT
Objective: To establish an HPLC method for the simultaneous determination of cafferic acid and rosmarinic acid in Perilla frutescens leaves and Schizonepeta tenuifolia. Methods: The anaiysis was carried out on an Agilent C18 column (250 mm × 4.6 mm, 5 μm), and the mobile phase was composed of actonitrile and 0.1% phosphoric acid aqueous with gradient elution. The detection wavelength was 327 nm. The flow rate 1.0 mL/min at column temperature of 30 ℃. Results: Under the chromatographic condition, cafferic acid and rosmarinic acid were completely separated and other components had no effect on their determination. It showed a good linearity in the range of 0.69-22.12 and 3.65 -116.92 μg/mL. The average recoveries of P. frutescens leaves were 102.9% and 105.6%, and the RSD values were 1.8% and 1.9%. The average recoveries of S. tenuifolia were 101.2% and 101.0% and the RSD values were 2.4% and 1.9%. Conclusion: The established method is rapid, sensitive, accurate, and the test component peak separation degree is good, and could be used for the simultaneous determination of cafferic acid and rosmarinic acid in P. frutescens leaves and S. tenuifolia, which provides a scientific basis for the quality evaluation of P. frutescens leaves and S. tenuifolia.
ABSTRACT
Objective: To establish an HPLC method for the simultaneous determination of cafferic acid, forsythoside A, forsythoside B, rutin, hyperoside, forsythin, and arctigenin in Forsythia suspensa. Methods: The analysis was carried out on an Inertsil ODS-3 C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was composed of acetonitrile and 0.2% phosphorie acid aqueous with gradient elution. The detection wavelength was set at 275 nm. The flow rate was 1.0 mL/min at column temperature of 30 °C. Results: Cafferic acid, forsythoside A, forsythoside B, rutin, hyperoside, forsythin, and arctigenin were well separated by this method, and showed a good linearity in the ranges of 18.24-91.20, 5.88-29.40, 132.60-663.00, 8.34-41.70, 1.96-9.80, 7.60-38.00, and 11.34-56.70 μg/mL, respectively. The average recoveries of the seven components were 97.7%, 96.7%, 102.6%, 101.3%, 93.2%, 91.8%, and 96.7% and the RSD values were 2.3%, 1.4%, 2.4%, 2.2%, 1.0%, 1.0%, and 1.3%, respectively. Conclusion: The established method is accurate, reliable, and could be used for the simultaneous determination of the seven components in F. suspense, which provides a scientific basis for the quality evaluation of F. suspense.