ABSTRACT
Objective To investigate the chemical constituents in the root of Camellia nitidissima var. longistyla. Methods The chemical constituents were separated and purified by silica gel, sephadex LH-20 column chromatography, preparative HPLC and so on. Their structures were determined by physical constants and spectral analyses. Results Sixteen compounds were obtained and identified as kaempferol (1), 3,5,6,7,4'-pentahydroxyflavone (2), 6-hydroxykaempferol (3), (-)-epiafzelechin (4), (-)-epicatechin (5), kaempferol-3-O-β-D-glucopyranoside (6), α-spinasterol (7), β-daucosterol (8), 5,7,3',4'-tetramethoxyflavone (9), 7,3',4'-trimethoxy-5- hydroxyflavone (10), 5,7,3',4',5'-pentamethoxyflavone (11), 3-O-acetyl oleanolic acid (12), n-tetratriacontanol (13), β-amyrin (14), α-spinasterol-3-O-β-D-galactopyranoside (15), and rutin (16). Conclusion All compounds are isolated from the plant for the first time. Compounds 2, 3, and 9-11 are isolated from the plants of Camellia for the first time.