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The study uses combining allergen preparation process of Coca and Sutherland method to prepare feather allergen with efficiency of 1.87g%. The allergen composes 16 amino acids, among that cyclic ones are of 19.25% and total nitrogen protein levels are 11,940 PNU (1/500 solution). The produced allergen has low toxicity, so it is safe for use
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Allergens , Candida albicansABSTRACT
Objective:To find the influence of the immuno-suppressive drug on the incidence and genotype of oral Candida albicans.Methods:51 patients using immuno-suppressive drug for 1 to 3 months were enrolled,the controls were 26 health subjects.Oral rinse samples from the patients and the controls were assessed for the growth of yeast.The liquid samples were plated on CHROMagar Candida.The isolates were defined to the species level and the genotypic subgroups by PCR method.PCR results were proved by sequencing.Results:The incidence of Candida albican in the patients and controls was 20% and 7.69% respectively.The genotype of the Candida albican in the controls is A subgroup,that in the patients included A,B and C subgroups.Conclusion:Long term of immuno-suppressive drug use can influence the genotype and incidence of oral Candida albican.
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0.05 ),the O- 2 production increased significantly (P
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Objective:To study the cytotoxic effects of granulysin on Candida albicans.Methods: Candida albicans were cultured with different concentrations of granulysin peptides and the colonies of Candida albicans on Sabouraud dextrose agar were calculated.Broth microdilution method was used to determine the minimum inhibitory concentrations(MIC) of granulysin and fluconazole on Candida albicans.Results: When granulysin peptides 2 and 3(corresponding to G2 and G3 peptides) were at 20 ?g/ml,the average colonies of Candida albicans decreased from 838 and 927 to 203 and 218,respectively;G1,G4 and G5 did not reduce the average colony of Candida albicans even at 40 ?g/ml.Three strains of Candida albicans were sensitive to granulysin,with their MICs being 26,22,29 ?g/ml,and their MICs to fluconazole were 4,20 and 128 ?g/ml.Conclusion: Granulysin has cytotoxic effects on Candida albicans and is one of the natural anti-fungi proteins in human body;it has a promising future for anti-fungi drug development.