Modeling and optimization of lipase-catalyzed synthesis of cholesteryol vinyl hemi-sebacate by response surface methodology / 中国药学杂志

OBJECTIVE: To model and optimize the reaction conditions of enzymatic esterification of cholesterol with diethenyl decanedioate in isooctane by response surface metodology (RSM).

Lipase-catalyzed synthesis of cholesteryl vinyl hemi-sebacate for selective targeting of liposomes in organic media / 中草药

Objective: To synthesize cholesteryl vinyl hemi-sebacate from cholesterol and divinyl sebacate in non aqueous phase. Methods: TLC, MS, and NMR were used to identify the structure of production; the technological conditions of enzymatic esterification were determined through orthogonal test. Results: The best condition: isooctane 5 mL, reaction temperature 35°C, reaction time 24 h, Candida rugosa Lipase 10 mg/mL, molar ratio of cholesterol to divinyl sebacate 1:6. Conclusion: The highest esterification rate is above 95%.