ABSTRACT
Objective: To study the inhibitory effect of rubusoside on the growth of Streptococcus mutans. Methods: S. mutans was added to each group, cultured in anaerobic incubator with constant temperature, and the pH value was measured and compared. Microbial adhesion to hydrocarbons method was used to test the adhesion and cell surface hydrophobicity. Centrifugation was followed, Bradford method and Neson-samogyi method were used to measure the total contents of protein and reducing sugar and to calculate the viability of glucosyltransferase (GTF). Anihrone method was used to measure the contents of water-insoluble glucans (WIG). Results: There were highly significant differences among the sweet tea groups, the experiment groups, and control groups on the pH value, adhesion, cell surface hydrophobicity, GTF activity, and total content of WIG (P < 0.01). Conclusion: Rubusoside has the strong inhibition on the ability of acid production, adhesion, cell surface hydrophobicity, GTF activity, and the synthesis of WIG of S. mutans.
ABSTRACT
Aim: To evaluate if dietary fermentable carbohydrate consumption associates with caries experience of youths and adults when assessed by a scoring system of putative cariogenicity of the foods. Methods: Two hundred and seventy five subjects (12 to 67 years-old) were examined to assess caries experience by the DMFT Index (decay missing and filled teeth) and radiographs. Subjects filled a weekly diet diary registering the type, frequency and time of consumption of foods. An arbitrary scoring system was used to determine cariogenic potential of the diet based on food consistency frequency and occasion of consumption. Results: DMFT index significantly increased with age (p<0.001). Females showed higher cariogenic potential than males (p=0.04). When subjects were divided into four DMFT categories, no differences were detected in cariogenic potential of the diet in any caries group. No correlation between caries experience and cariogenic potential of the diet could be found. Conclusions: Arbitrarily assigned cariogenicity of dietary fermentable carbohydrates does not seem to associate with caries experience, when information was obtained through a cariogenicity scoring system.
Objetivo: Evaluar si el consumo de carbohidratos fermentables se asocia con la experiencia de caries en jóvenes y adultos, cuando es medido por un sistema de asignación de puntajes a los alimentos según su presunta cariogenicidad. Metodología: 275 sujetos (12 a 67 anos) fueron examinados para determinar su experiencia de caries mediante el índice COPD (dientes cariados, obturados y perdidos) y radiografías. Los sujetos llenaron un diario dietético semanal. Se utilizó un sistema arbitrario para asignar puntajes y determinar el potencial cariogénico de la dieta basado en la consistencia, frecuencia y ocasión de consumo. Resultados: Las mujeres mostraron mayor potencial cariogénico que los hombres (p=0,04). Cuando los sujetos fueron divididos en 4 categorías de COPD, no se detectaron diferencias en su potencial cario-génico en ningún grupo de caries. No se observó correlación entre la experiencia de caries y el potencial cariogénico de la dieta (p>0,05). Conclusiones: La cariogenicidad arbitrariamente asignada a los carbohidratos fermentables de la dieta no parece asociarse con la experiencia de caries en adultos.
Subject(s)
Humans , Dietary Carbohydrates , Adolescent , Adult , Dental Caries , Diet, Cariogenic , Sugars , Fermented Foods , ChileABSTRACT
Consumption of liquid oral medicine by frequently ill children, significantly increases the risk of tooth demineralization. In this study a survey was conducted wherein the pediatricians, pharmacist and medical representatives of Bhopal city were enquired about the most commonly prescribed liquid oral medicines for children. The four categories of medicines reported were: antipyretics/analgesics, antibiotics, antihistaminics/antitussives and multivitamins. Three samples from each category were taken. Sugar concentration was evaluated by Lane-Eynon general volumetric method and pH was measured by using digital pH meter (EU-TECH). High concentration of sugar was found in 50% of samples and 90% samples showed low pH. Sugar concentration was in a range of 20.6 to 68.2% and pH in a range of 3.8 to 8.5. As sugar in medication is a potential threat to teeth, therefore, alternatives including sugar substitute should be considered.