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OBJECTIVE@#This study was aimed at investigating the carrier rate of, and molecular variation in, α- and β-globin gene mutations in Hunan Province.@*METHODS@#We recruited 25,946 individuals attending premarital screening from 42 districts and counties in all 14 cities of Hunan Province. Hematological screening was performed, and molecular parameters were assessed.@*RESULTS@#The overall carrier rate of thalassemia was 7.1%, including 4.83% for α-thalassemia, 2.15% for β-thalassemia, and 0.12% for both α- and β-thalassemia. The highest carrier rate of thalassemia was in Yongzhou (14.57%). The most abundant genotype of α-thalassemia and β-thalassemia was -α 3.7/αα (50.23%) and β IVS-II-654/β N (28.23%), respectively. Four α-globin mutations [CD108 (ACC>AAC), CAP +29 (G>C), Hb Agrinio and Hb Cervantes] and six β-globin mutations [CAP +8 (C>T), IVS-II-848 (C>T), -56 (G>C), beta nt-77 (G>C), codon 20/21 (-TGGA) and Hb Knossos] had not previously been identified in China. Furthermore, this study provides the first report of the carrier rates of abnormal hemoglobin variants and α-globin triplication in Hunan Province, which were 0.49% and 1.99%, respectively.@*CONCLUSION@#Our study demonstrates the high complexity and diversity of thalassemia gene mutations in the Hunan population. The results should facilitate genetic counselling and the prevention of severe thalassemia in this region.
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Humans , beta-Thalassemia/genetics , alpha-Thalassemia/genetics , Hemoglobinopathies/genetics , China/epidemiology , High-Throughput Nucleotide SequencingABSTRACT
Objective@#To develop a system for rapid detection of JAK2 V617F mutation among patients with myeloproliferative diseases.@*Methods@#Specific primers and TagMan probes were designed for the mutant and wild type alleles based on the principle of real-time PCR. A complete system including the method for detection and product for quality control were established through the evaluation of sensitivity and accuracy of the method, double-blind trial, and preparation of negative and positive controls through site-directed mutagenesis and molecular cloning.@*Results@#A system for rapid detection of the JAK V617F mutation has been developed. Compared with Sanger sequencing, the sensitivity and specificity of the method have both reached 100%. Meanwhile, 1000 normal samples and 1 case with the JAK2 V617F mutation were detected, which gave a population rate of 1‰.@*Conclusion@#The system was fast, accurate, cheap, high throughput, and easy to use. It can be utilized as a routine test. Although the JAK2 V617F mutation is rare in the population, it should be screened among myeloproliferative neoplasm patients.
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Objective To provide evidences which are for clinicians to formulate effective prevention and treatment measures by analyzing the perinatal pregnant women vaginal group B streptococcus (GBS)infection and adverse pregnancy outcomes. Methods From January 2013 to February 2015,the vaginal secretions were sampled from 795 cases of perinatal pregnant women for culture identification of GBS,the final results were statistical analyzed.Results In the 795 pregnant women ca-ses,there were 256 GBS carriers cases,the carrier rate was 32.2%.There was statistically significance (χ2 = 19.095,P <0.01)between less than 30 years old group (28.9%)and greater than or equal to 30 years old group (42.3%).The differ-ent incidence rate of clinical symptoms between GBS negative cases and GBS positive cases (18.8% vs 8.0%)was also had statistically significance (χ2 =39.514,P <0.01).The antimicrobial resistant rates of ten kinds of antibacterial drugs (vanco-mycin,linezolid,penicillin,ampicillin,ceftriaxone,nitrofurantoin,levofloxacin,clindamycin,erythromycin and tetracycline) was 0,0,0.6%,3.1%,6.6%,9.6%,21.9%,23.8%,29.9% and 58.1% respectively.The positive rate of D-bacteriostatic ring experiment was 23.9%.Conclusion The carrier rate of perinatal pregnant women GBS was higher in this region,and the elderly were easier to be infected.Perinatal pregnant women infected with GBS to vancomycin and rina thiazole amine and penicillin and ampicillin and ceftriaxone and with nitrofurantoin because of the sensitive rate is high.
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Objective To analyze the positive rate of HBsAg in different population at different periods after widely using Hepatitis B Vaccine and provide strategy for Hepatitis B Control.Methods Using ELISA method to detect the HBsAg carrier in polulation under 18 years old in local area.Results After six year investigating,the HBsAg carrier rate in tested population decreased.For 0-4,4-8,8-12,12-15,15-18 age group,the carrier rate were 3.76% vs 0.30%,4.74% vs 1.39%,5.59% vs 2.52%,8.51% vs 3.03%,and 12.58% vs 2.98% in 2000 and 2006.Conclusion The reduction in carrier rate had a direct relationship with Hepatitis B Vaccine inoculation.Hepatitis B Vaccine had important effect to Hepatitis B control.We should gradually intensify Hepatitis B Vaccine inoculation.It is not only for newborn but also for adult.
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0.05).The results differentiated by Sysmex XS-800i showed excellent correlation with those by handwork.Conclusion The performance of the Sysmex XS-800i automated hematology analyzer is satisfied.
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In this paper,the candida carrier rate of 1996 healthy infants and juveniles of the Han,Dai and Zang nationalities was reported.The specimens were taken from the tongue,cheek mucosa,stool,urine,finger web and vagina.The carrier rate of C.albicans in the mouth was significantly higher than that in the other regions. C.albicans at the tongue of the Han,Dai and Zang people was 4.5%,5.3%, 1.07% respectively.Therefore,C.albicans' residence in the mouth was probably an important source of the internal infection of candidiasis.Of the 1996 healthy individuals none had C.albicans isolated from the urine.The carrier rate of C. albicans isolated from the vagina was only 0.15% in the Han and zero in the Dai and Zang.The carrier rate of candida was least in the newborn.Our data also suggested that the carrier rate of candida at the tongue was much higher than that at the cheek mucosa.Regard must be paid when isolating fungi from the mouth.
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The Campylobacter carrier rate of the bile was 23. 64%,14. 29%,7. 14%,6. 64%,5.88%, 5.55% and 3. 63% in the quails, cats, dogs, pigs, chickens, ducks and oxen respectively. The survival time of Campylobacter in the bile in vitro ranged from 4 to 7 weeks. The Campylobacter carrier rate of the intestinal content was higher than that of the bile in all the domestic animals and fowls. It is believed that the domestic animals and fowls may be an important source of Campylobacter infection in human beings according to our findings.
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Research study of Hepatitis B infection has been much advanced recently and the incidence of the Hepatitis B infection, such as acute viral hepatitis, chronic active hepatitis, chronic persistant hepatitis, acute fulminating hepatitis and carrier states are increasing significantly. The authors evaluated HBsAg and AntiHBs in the child age group and the results are summarized as follows: Of 271 children who were admitted during the period of June 1, 1981 to August 31, 1981, 15(5.9%) were HBsAg positive and 24(8.86%) were antiHBs positive. The highest incidence of HBs Ag among the children was school age group. The highest incidence of AntiHBs was newborn age group. Of 92 children who were living in the orphanage, 12(13.0%) were HBsAg positive and 8 (8.71%) were AntiHBs positive. There was no significant difference in comparison by each room.
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Child , Humans , Infant, Newborn , Australia , Carrier State , Hepatitis , Hepatitis B , Hepatitis B Surface Antigens , Hepatitis, Chronic , Incidence , Orphanages , PrevalenceABSTRACT
Research study of Hepatitis B infection has been much advanced recently and the incidence of the Hepatitis B infection, such as acute viral hepatitis, chronic active hepatitis, chronic persistant hepatitis, acute fulminating hepatitis and carrier states are increasing significantly. The authors evaluated HBsAg and AntiHBs in the child age group and the results are summarized as follows: Of 271 children who were admitted during the period of June 1, 1981 to August 31, 1981, 15(5.9%) were HBsAg positive and 24(8.86%) were antiHBs positive. The highest incidence of HBs Ag among the children was school age group. The highest incidence of AntiHBs was newborn age group. Of 92 children who were living in the orphanage, 12(13.0%) were HBsAg positive and 8 (8.71%) were AntiHBs positive. There was no significant difference in comparison by each room.