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Natural colorants derived fom plant materials have gained increasing popularity due to their non toxic nature. pigment extraction from the florets is normally done by Soxhlet extraction, maceration, and hydro distillation are conventional methods that have been widely used in industry and laboratory .phytochemical analysis of safflower florets revealed the plant presence of high amount of Carthamin and carthamidin.
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Objective To study the effect of Carthamin Yellow (CY) on cell proliferation, apoptosis, migration and invasion ability of breast cancer and its related molecular mechanisms. Methods CCK-8 assay was used to detect cell viability of MDA-MB-231 human breast cancer cells by different concentrations of CY at different time;flow cytometry was used to test the apoptosis rate of MDA-MB-231 cells treated by different concentrations of CY and transwell assay was used to investigate the effect of various concentrations of CY on MDA-MB-231 cell migration and invasion.After the intervention of different concentrations of CY on MDA-MB-231 cells, apoptosis-related protein Cleaved-Caspase-3, survival protein p-Akt and metastasis-related protein MMP2 were detected by western blot. Results (1) CY could inhibit the proliferation of MDA-MB-231 cells in a dose-and-time-dependent manner. (2) CY significantly promoted the apoptosis of breast cancer cells ( <0.01) . (3) CY could decrease the expression of p-Akt and increase the expression of Cleaved-Caspase-3. (4) CY impaired migration and invasion of MDA-MB-231 cells ( <0.01), and can inhibit the expression of MMP2. Conclusion CY could promote the apoptosis of breast cancer cells through activation of apoptosis signaling, and can inhibit breast cancer cell metastasis by suppressing MMP2. And CY may be a potential therapeutic drug for human breast cancer.
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Objective: To establish microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS) method for the determination of 13 kinds of metals (Pb, Cd, Hg, As, Cu, Fe, Mn, Zn, Ba, Al, Co, Cr, and Ni) in 10 kinds of Chinese materia medica (CMM) injections which are Carthamin yellow for injection, Breviscapine for injection, Urokinase for injection, Lugua Polypeptide for injection, Xuesetong for injection, Shuanghuanglian for injection, Tanreqing Injection, Salvia miltiorrhiza Injection, Yinxing Damo Injection, and Danhong Injection). Methods: HNO3 and H2O2 are as acid digestion system; Samples were conducted pretreatment by microwave digestion instrument; Sc, In, Rh, and Re were used as internal standard elements, 13 kinds of elements were determined by ICP-MS at the same time. Results: Different kinds of the elements had a good linear relationship (r>0.999); The limit of detection was range from 0.008 to 0.069 μg/L, the average recovery rate was range from 93.61% to 100.79%; RSD values of the repeatability and precision were all less than 5%, The As and Cd in 10 kinds of CMM injections were all less than the limit of detection, Pb was ranged from 0.109 to 0.017 mg/kg, Cu was ranged from 0.016 to 0.135 mg/kg, Hg was ranged from 0.005 to 0.018 mg/kg, Fe was ranged from 0.335 to 2.081 mg/kg, Mn was ranged from 0.021 to 0.699 mg/kg, Ba was ranged from 0.009 to 0.028 mg/kg, Zn was ranged from 0.005 to 3.186 mg/kg, Al was ranged from 0.518 to 10.012 mg/kg, Co was ranged from 0.003 to 0.028 mg/kg, Cr was ranged from 0.006 to 0.179 mg/kg, Ni was ranged 0.005 to 1.187 mg/kg, five kinds of heavy metals which were needed to control were safe according to Chinese Pharmacopoeia (2010), the contents of Fe, Mn, Zn, and Al were not too high, but their safe limits were needed to be further in-depth researched and analyzed. Conclusion: This method is simple and fast, and has good accuracy and high precision, so it can be recommended for the determination of metal elements in CMM injections and provide the reference for its quality control.
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OBJECTIVE:To establish an RP-HPLC analysis of Carthamin in mice and to study its pharmacokinetics.METHODS:The serum concentration of Carthamin was determined by RP-HPLC.The blood concentration-time curve was established and the main pharmacokinetic parameters were computed.RESULTS:The linear range of Carthamin was 0.558～55.8 ?g?L-1(r=0.999 2),with the lowest limit of detection at 0.005 ?g?L-1Carthamin in vivo assumed two-compartment model and rapid absorption.CONCLUSION:The proposed method is simple,sensitive and reproducible,and it met the standard for pharmacokinetic study.
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Objective To provide the basis for determining the suitable collecting time of safflower. Methods To measure the contents of saflor yellow-A in safflower crude drug from different collecting time in one day by HPLC. Results Only saflor yellow-A has big change. By experimental analyzing samples of different year and region, A bright time collecting in the flok, the content of saflor yellow-A in medicine assured higher. Conclusion A bright time collecting has some scientific basis.