ABSTRACT
Objective To study the anti-platelet aggregation activity of Dendrobium denneanum. Methods A total of 21 rats were randomly divided into the extract 1 group, the extract 2 group, the extract 3 group, the extract 4 group, the extract 5 group, the extract 6 group and the positive control group, with 3 in each group. While 9 guinea pigs were randomly divided into the extract 5 group, the extract 6 group and the positive control group, 3 guinea pigs per group. The platelet aggregation was induced by adenosine triphosphate (ADP) in vitro in rats and arachidonic acid (AA) in vitro in guinea pigs. Plate let rich plasma (PRP) was collected. Each group of PRP was added with different concentrations of Dendrobium denneanum extract 1, 2, 3, 4, 5, 6 or aspirin to intervene. The maximal aggregation rate were detected in 5 min, and to calculate the inhibition rate of platelet aggregation and half of each test substance inhibitory concentration (IC50). Asp was detected in 5 min. The above steps were repeated three times. The inhibition rate of platelet aggregation and the IC50 of each subject were calculated. The maximum aggregation rate of the drug delivery group and the blank control group was compared to evaluate the antithrombotic activity of the Dendrobium with two different induction methods. Results Compared with the blank control group, the maximal aggregation rate induced by ADP of the extract 12.399 mmol/L dose group, the extract 28.96 mmol/L dose group, the extract 317.505 mmol/L dose group , the extract 57.836, 3.918 mmol/L dose group, the extract 60.755, 0.377 mmol/L dose group (32.57% ± 0.28% vs. 45.20% ± 2.64%), (16.66% ± 3.67% vs. 39.37% ± 8.05%), (0.00% ± 0.00% vs. 32.52% ± 3.05%), (1.11% ± 1.93%, 7.49% ± 9.47% vs. 43.54% ± 10.68%), (8.66% ± 7.50%, 22.71% ± 8.24% vs. 40.65% ± 6.61%) significantly decreased (P<0.05 or P<0.01). Compared with the blank control group, the maximal aggregation rate induced by AA of the extract 57.836 mmol/L dose group, the extract 60.377 mmol/L dose group (0.00% ± 0.00% vs. 52.97% ± 5.49%), (0.00% ± 0.00% vs. 53.47% ± 6.04%) significantly decreased (P<0.05 or P<0.01). The IC50 of extracts 5 and 6 were 3.143 and 0.100 mmol/L, respectively. Conclusions There were many kinds of ingredients in Dendrobium denneanum which had anti-platelet aggregation activity. Dendrobium denneanum had positive effects on thrombotic diseases.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between transformation growth factor (TGF- B) polymorphisms and IgA nephropathy and the therapeutic effect of dendrobium on IgA nephropathy.</p><p><b>METHODS</b>Polymerase chain reaction- restriction fragment length polymorphism (PCR- RFLP) and direct sequencing were used for analysis of 118 patients with IgA nephropathy from core families in Guangxi Zhuang Autonomous Region. The imbalanced transfer of TGF iso1-509 C/T in the affected offsprings was observed by transfer imbalance test and HRR analysis. The TGF-B genotype of the patients and the core family members were detected. The therapeutic effects of Dendrobium candidum combined with hormone and ACEI/ARB treatments were evaluated by observing the patient's urine protein (24 hUpr), serum albumin (ALB), creatinine (Scr) and urea nitrogen (BUN) levels.</p><p><b>RESULTS</b>In the 118 patients with IgA nephropathy, we identified TGF-B 1 promoter -509C/T genotype CC in 32 (27.1%) cases, CT in 58 (49.2%) cases, and TT in 28 (23.7%) cases. In the core family of the patients, CC genotype was found in 33 (28.0%) cases, CT in 55 (46.6%) cases, and TT in 30 (28.0%) cases. The treatments significantly lowered 24 hUpr, Scr, and BUN levels ( > 0.05) in patients with CC genotype, significantly lowered 24 hUpr and BUN levels in patients with CT genotype ( < 0.05), and significantly lowered 24 hUpr and BUN level and increased ( < 0.05) ALB level ( < 0.01) in patients with TT genotype.</p><p><b>CONCLUSIONS</b>There is no significant correlation between TGF-B promoter - 509C/T polymorphism and IgA nephropathy. The patients with CC genotype are sensitive to the treatments with hormone and ACEI/ ARB and show a stronger response to combined treatments with dendrobium.</p>
ABSTRACT
OBJECTIVE: To study on the effect of different extracting methods on the content determination of polysaccharide from Caulis Dendrobii.METHODS: Polysaccharide was extracted from Caulis Dendrobii respectively by reflux extraction,improved reflux extraction,ultrasound wave extraction and ultrasonic-assisted thermal reflux extraction.The content of polysaccharide was determined by phenol-sulfuric acid method.RESULTS: The determined results using these methods were as follows:(13.473?0.634)%,(15.692?0.743)%,(11.87?0.536)% and (16.29?0.407)%.CONCLUSION: The content determination of polysaccharide from Caulis Dendrobii was associated with the extraction method.The polysaccharide from Caulis Dendrobii could be extracted more thoroughly and rapidly by ultrasonic-assisted extraction technology.Therefore this method could be used to pretreat the sample in content determination of Caulis Dendrobili polysaccharide.