ABSTRACT
Escherichia coli is a type of bacteria that has evolved yearly, such as resistance to various antibiotics. Honey consumption can improve the quality of individual health. It has the potential to inhibit the growth of Escherichia coli. This study aimed to characterize the Escherichia coli ATCC 33218, and to investigate the inhibitory activity of Ceiba' honey against this strain. We obtained Ceiba' honey from the honey farm of Ceiba petandra. The antibiotic sensitivity tests revealed that the strain used in this study is not ESBL strain. Even it is resistant to Ampicillin, Amoxycillin, Penicillin, and Oxacillin and is sensitive to Cefotaxime. This study runs inhibition zone, MIC, MBC, and time-kill growth experiments to evaluate the inhibitory activity of Ceiba' honey. It showed a strong inhibition zone. Its MIC and MBC were 30 and 40%, respectively. The time-kill growth experiments revealed its inhibition by lowering the lag phase, slowing the growth rate, and decreasing the biomass yield of strain. In conclusion, Escherrichia coli ATCC 33218 is not an ESBL strain. Furthermore, Ceiba' honey has inhibitory and killing power against Escherichia coli ATCC 33218.
ABSTRACT
Background: The Indonesian people were used the leaves of silk cotton tree (Gossypium arboreum L.) and cotton tree (Ceiba pentandra Gaertn.) as an antipyretic, empirically. There is no scientific evidences on the antipyretic activity of these plants. Objective: The aim of this study is to determine the better antipyretic activity of the leaves extract of G. arboreum and C. pentandra in Swiss mice as an animal model. Methods: The steps of the study consisted of extraction, phytochemical screening, and antipyretic activity assay on Swiss mice which induced by 20% yeast suspension. Results: The results showed that the leaves extract of G. arboreum and C. pentandra have antipyretic activity. The effective dose of antipyretic activity for the leaves extract of G. arboreum and C. pentandra is 1120 mg/kg and 189 mg/kg, respectively. Conclusion: The antipyretic activity of C. pentandra leaves extract better than G. arboreum leaves.
ABSTRACT
Aims: To evaluate the protective effects of methanol extract of Ceiba pentandra leaves on indomethacin and ethanol induced gastric ulcer and on oxidative stress indices of alloxan-induced diabetic rats. Study Design: Extraction and administration of graded doses of the extract Place and Duration of Study: Department of Biochemistry, University of Nigeria, Nsukka. Enugu State, Nigeria, between May, 2011 and October, 2011. Methodology: Extraction of Ceiba pentandra leaves was done using methanol. Twenty adult rats divided into five groups of four rats each were used for each of the ulcer studies. Gastric ulceration was induced in the rats by oral administration of indomethacin (50 mg/kg) and 95% ethanol (0.5 ml) thirty minutes after extract treatment, and the animals sacrificed 8 h later. For the diabetes study, thirty (30) albino rats divided into six (6) groups of five (5) rats each were used. Diabetes was induced by i.p injection of alloxan monohydrate (150 mg/kg) in overnight-fasted animals and the animals treated with varied doses (100, 200 and 400 mg/kg) of the extract for two weeks. Serum obtained from the diabetic rats was used for the determination of lipid profile and liver marker enzymes. Results: Significant and dose dependent ulcer inhibition (70, 82 and 84 %; 19, 53, and 58 % for 100, 200 and 400mg/kg of the extract respectively) was produced in all the extracttreated groups for the ulcer models used. There were significant decreases (p<0.05) in fasting blood glucose levels, liver marker enzymes, total cholesterol, low density lipoprotein and triacylglycerides in the serum of extract-treated groups compared with that of the diabetes-untreated group. Conclusion: The findings in this study show that methanol extract of Ceiba pentandra leaves possesses potent anti-ulcerogenic and anti-oxidative properties and has potential for use as an herbal remedy for the treatment of gastro-intestinal ulcer and management of diabetes.