ABSTRACT
AlM:To investigate the changes of corneal endothelial cells and tear film in diabetic cataract patients after operation. METHODS: Totally 88 patients (88 eyes) with diabetic cataract (study group) treated in our hospital from June 2016 to June 2017 were retrospectively analyzed, and 100 patients (100 eyes) with senile cataract (control group) were selected. Patients of two groups underwent phacoemulsification and foldable intraocular lens implantation and followed up for 3mo. The corneal endothelial cell density, endothelial cell coefficient of variation and hexagonal cell proportion were measured by TOPCON SP - 3000P non- contact corneal endothelial tester before and after operation in two groups. The tear break-up time (BUT), basal tear secretion test (S︳t) and corneal fluorescein test (FL) in two groups were observed. RESULTS: There was no difference in corneal endothelial cell density between the two groups before treatment and 7d after treatment (P>0.05). The corneal endothelial cell density in the study group was significantly lower than that in the control group (3mo after treatment) (P < 0. 05). The density of corneal endothelial cells decreased significantly in two groups at each time points after treatment (P<0.05). The variation coefficient of corneal endothelial cells showed significant difference between groups at 7d and 3mo after treatment (P< 0. 05). The variation coefficient levels of corneal endothelial cells in both groups increased after treatment (P<0.05). The ratio of hexagonal cells decreased after treatment (P<0.05),and the ratio of hexagonal cells at 7d and 3mo after treatment in study group was significantly lower than that of the control group (P<0.05). Before treatment,there was no difference in BUT, S︳t and FL between the two groups (P>0.05). BUT and FL between the two groups at 7d after treatment had no significant difference (P>0.05), while the S︳t of the study group was significantly lower than that of the control group (P<0.05). BUT and S︳t of the study group were significantly lower than those of the control group at 3mo after treatment(P<0.05),with no difference in FL between the two groups (P>0.05). After treatment,BUT,S︳t and FL were improved in the two groups (P<0.05). CONCLUSION: Phacoemulsification has some damage to corneal endothelial cells and destroys the stability of tear film, corneal endothelial cells are damaged more severely and recover slowly after operation especially in diabetic cataract patients.
ABSTRACT
Abstract This study aimed to analyze the antiproliferative and genotoxic potential of synthetic food flavorings, nature identical passion fruit and artificial vanilla. This assessment used root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and analyzed with optical microscope at 400× magnification, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. Doses of 0.2 mL at ET 48 h; 0.4 and 0.6 mL at ET 24 and 48 h of passion fruit flavor, and the three doses of the vanilla flavor at ET 24 and 48 h significantly reduced the cell division rate in the meristems of roots, proving to be cytotoxic. Doses of 0.2; 0.4 and 0.6 mL of the passion fruit additive, and the three doses of vanilla tested, in the two exposure times, induced mitotic spindle changes and micronuclei formation in the cells of the test organism used, proving to be genotoxic. Therefore, under the studied conditions, flavoring solutions of vanilla and passion fruit, marketed nationally and internationally, significantly altered the functioning of the cell cycle in root meristem cells of A. cepa.
Resumo Neste trabalho teve-se por objetivo analisar o potencial antiproliferativo e genotóxico de aromatizantes alimentares sintéticos, idêntico ao natural de Maracujá, e artificial de Baunilha. Esta avaliação foi realizada por meio das células meristemáticas de raízes de Allium cepa L., nos tempos de exposição de 24 e 48 horas e nas doses de 0,2; 0,4 e 0,6 ml. As raízes foram fixadas em solução de Carnoy, hidrolisadas em ácido clorídrico e coradas com orceína acética. Analisou-se, em microscópio óptico em aumento de 400×, 5.000 células por grupo tratamento, e utilizou-se o teste estatístico Qui-quadrado a 5% para análise dos dados. Verificou-se que as doses de 0,2 ml, no TE 48 h; 0,4 e 0,6 ml, nos TE 24 e 48 h, do aromatizante de Maracujá, e as três doses analisadas, nos TE 24 e 48 h, do aditivo de Baunilha reduziram significativamente o índice de divisão celular dos meristemas de raízes, mostrando-se citotóxicas. As doses 0,2; 0,4 e 0,6 ml do aditivo de Maracujá, e a de 0,6 ml do aromatizante de Baunilha, nos dois tempos de exposição considerados, induziram alterações de fuso mitótico e micronúcleos as células do organismo de prova utilizado, mostrando-se genotóxicas. Portanto, nas condições analisadas, as soluções aromatizantes de Baunilha e Maracujá, comercializadas nacional e internacionalmente, alteraram significativamente o funcionamento do ciclo celular das células meristemáticas de raízes de A. cepa.
Subject(s)
DNA Damage , Meristem/drug effects , Micronuclei, Chromosome-Defective , Food Additives/toxicity , Cell Nucleus , Plant Roots/drug effects , Onions/drug effects , MitosisABSTRACT
Adenomatoid odontogenic tumor (AOT) has a limited biological profi le and been an attention-grabbing tumor for a century for its origin. Though described earlier, it was widely accepted after Harbitz from Norway reported about this uncommon benign tumor in 1915. There has been a long debate as whether this tumor is a hamartoma or a neoplasm. Here, we present a case of AOT in a 20-year-old female with details of clinical, radiological and histological features along with clear cell changes, signifying AOT to be more aggressive in nature than assessed from earlier literature. Thus, we did an extensive search of PubMed literature on AOT with all its histopathological features associated until date to fi nd the report of clear cell changes yet.