ABSTRACT
OBJECTIVE: To establish an RP-HPLC analytical method for simultaneous determination of crysoeriol and centaureidin in the aeries parts of Echinops integrifolius. METHODS: The separation was achieved on a Kromasil C18 column (4.6 mm×250 mm, 5 μm)at 30℃ using acetonitrile-water-acetic acid solution (35∶65∶2) as mobile phase at a flow rate of 1.0 mL·min-1 and 350 nm as the detection wavelength. RESULTS: The linear ranges of crysoeriol and centaureidin were 0.4-2.4 mg·L-1(r=0.999 8), 0.6-2.6 mg·L-1 (r=0.999 9), respectively. The average recoveries (n=6) were 91.6% and 92.7%, and RSDs were 1.37% and 1.08% respectively. CONCLUSION: The methodology validation shows that this method is accurate, simple and reliable, which is applicable for the simultaneous determination of two flavonols crysoeriol and centaureidin in the aeries parts of Echinops integrifolius.