ABSTRACT
Objective To observe whether the polypeptide monoclonal antibody induced by human papillomavirus (HPV) major capsid protein (L1) C-terminal conserved sequence polypeptide can be applied to detect HPV in cervical exfoliated cells. Methods Two copies of cervical exfoliated cells specimens were collected from the gynecology clinic. The Second Clinical Medicine College. China Three Gorges University: one was subjected to detection via Human Papillomavirus Genotyping Diagnosis Kit in the clinical laboratory of hospital. and the other was subjected to detection of HPV L1 via immunohistochemistry (IHC) with the mouse monoclonal antibody induced by HPV L1 C-terminal conserved sequence polypeptide. The detection results of HPV of the two methods were analyzed. Results In a total of 384 cervical exfoliated cell samples. 63 were found positive for HPV by the Human Papillomavirus Genotyping Diagnosis Kit. with a positive rate of 16. 41% (63/384); while IHC identified 120 positive cases, with a positive rate of 31. 58% (120/380), being significantly higher than that of the former method (P<0. 05). Conclusion It is indicated that HPV L1 polypeptide mouse monoclonal antibody has a better detection performance for HPV infection in cervical exfoliated cells compared with Human Papillomavirus Genotyping Diagnosis Kit. and t may have a future in developing kit for the prevention screening of cervical cancer.
ABSTRACT
Objective To review the HPV infection status of the females in Shantou in the past years.Methods 3 664 speci-mens of cervical exfoliated cells of the female patients with gynecological disease from 2013 to 2014 were selected to ampli-fied HPV gene and conducted genetypes detection.The infection rate and various subtypes of HPV were analyzed.All these patients were divided into 5 groups according to their ages and the infection status of each group were also discussed.Results 805 patients were infected with HPV (22%,805/3 664).The single infection type and the mixed infection type were both visible,HPV-16 was the most common single infection type,followed by subtypes HPV-52,43,58 and 56.HPV16+52 was the most common mixed infection type,followed by HPV16+33 and HPV43+52.The infection rates of the each group were differences (χ2 =27.08,P 25),No.2 (25 ~ 34)and No.5 group (< 55)were the high-risk groups of HPV infection.Conclusion The main genotypes of these women whose infection with HPV were HPV-16,52,43,58 and 56 in Shantou region,respectively.The females aged less than 35 years old and more than 55 years old both had high-risk for HPV infection.It has important significance to developed the vaccines which can efficiently prevent HPV-16,52,43,58,56 and reduce the cervical cancer incidence.
ABSTRACT
Objective To determine whether the polypeptide antibody induced by human papillomavirus (HPV)L1 C-terminal conserved sequence polypeptide can be used to detect HPV in cervical exfoliated cell specimens. Methods Cervical exfoliated cell specimens were collected and divided into two groups: one was subjected to nested polymerase chain reaction (Nest-PCR) for detection of HPV DNA, and the other was subjected to sandwich enzyme-linked immunosorbent assay (ELISA), in which rabbit anti-polypeptide purified antibody and mice anti-polypeptide antiserum were used to determine HPV L1 in specimens. The detection rates of HPV were compared between the two groups. Part of the specimens were used for immunocytochemistry detection. Results Nest-PCR with primer MY09/11 detected 30 HPV DNA positive specimens out of a total of 296 ones, with a detection rate of 11.15%. Then the HPV DNA negative specimens were further amplified with primer GP5+/6, and 51 (21.34%) more positive cases were identified, making the total detection rate of Nest-PCR being 30.11% (totally 81 cases). ELISA identified 91 positive cases out of 263 specimens, with a detection rate of 34.60%, which was not significantly different from that by Nest-PCR. Immunocytochemistry detection results displayed that the polypeptide antibody could specifically reveal cervical exfoliated cells infected with HPV. Conclusion The HPV L1 polypeptide antibody can satisfactorily detect the HPV infection in cervical exfoliated cells, indicating that HPV L1 polypeptide antibody has great potential in developing HPV detection kit for cervical cancer preventive screening.