ABSTRACT
Quality evaluation of Chinese medicinal decoction pieces is vital for the development of the downstream industries, and is an important channel for implementing the strategy of "higher quality, higher price, and priority for the high quality" for traditional Chinese medicine. At the moment, the quality of Chinese medicinal decoction pieces is mainly evaluated based on chemical component examination. Considering the weak preliminary research foundation and poor research conditions, traditional experience-based evaluation is undervalued in the quality rating of Chinese medicinal decoction pieces. However, traditional experience is a summary of the quality of Chinese medicinal materials based on clinical experience, which thus can be a potential basis for the quality evaluation of the decoction pieces. It is a challenge in the evaluation of Chinese medicinal decoction pieces to objectify the traditional experience-based evaluation from multiple aspects such as chemistry, effect, and characterization via modern techniques. Therefore, this study developed the "experience-ingredients-activity-electronic sensing" evaluation system for Chinese medicinal decoction pieces on the basis of experience-based assessment, chemical ingredients that can truly reflect the traditional experience, biological effect assessment, and electronic sensory evaluation, which is expected to quantify the traditional experience of quality evaluation of Chinese medicinal decoction pieces via chemistry, biology, and sensory simulation. The evaluation system can serve as a reference for clinical experience-based quality evaluation of Chinese medicinal decoction pieces.
Subject(s)
China , Drugs, Chinese Herbal , Electronics , Medicine, Chinese Traditional , Restraint, PhysicalABSTRACT
Objective: To establish an HPLC wavelength switching method for the simultaneous content determi- nation of verbascoside,paederosidic acid methyl ester,asperulosidic acid,asperuloside,calycosin7-O-β-D-glucopyran- oside,ononin,calycosin,formononetin and batatasinin Shenkangling granules(Radix Rehmanniae,Herba Hedyotis Diffusae,Radix Astragali,Rhizoma Dioscoreae,etc.). Methods: The Shenkangling granules were extracted with 50% aqueous methanol to prepare the test solution. The HPLC analysis was performed on thermostatic Agilent Eclipse XDB-C18 (250 mm×4.6 mm,5 μm)a 30℃,with the mobile phase consisting of acetonitrile-0.2% phosphoric acid solution at 0.9 ml/min flow rate in a gradient elution manner,and the detection wavelength was set at 334,238,254 and 270 nm, respectively. Results The above mentioned nine compounds showed good linearity in turn within the range of 3.09- 61.80,3.67-73.40,1.98-39.60,2.51-50.20,1.39-27.80,1.06-21.20,0.87-17.40,2.79-55.80,and 2.16-43.20 μg/ml, respectively,and their average recovery was 99.34%,100.03%,97.67%,98.63%,97.96%,96.93%,97.48%,99.87%, and 98.95% with the RSD of 0.88%,1.15%,1.28%,1.49%,0.99%,1.41%,1.37%,0.72%,and 1.25%,respectively.Conclusion: The method is easy to operate,repeatable,and can be used for quality control of Shenkangling granules.
ABSTRACT
Natural Calculus Bovis,one of the precious traditional Chinese medicines,is the dried gallstone in bovine gallbladder,bile duct or hepatic duct.It is one of the essential components of the formulation of a variety of traditional Chinese medicines.Due to high price and rareness of natural Calculus Bovis,Chinese researchers have developed artificial Calculus Bovis,cultured Calculus Bovis and Calculus Bovis Sativus as substitutes of it.They are mainly composed of bilirubin,bile acids,amino acids and inorganic elements.Due to the co-existence of natural Calculus Bovisa and its substitutes in the market,and the complex composition of them,identification,quality control and material basis study of them are important issues in recent years.Currently,the quality control of them mainly focus on cholic acid and bilirubin.In order to fully solve the quality control problem of natural Calculus Bovis and its substitutes,researches on testing techniques of other bile acids or other components have already started.In this paper,research progress on the chemical compositions,quality control and the test technology of bilirubin,bile acids,amino acids and other components of Calculus Bovis are reviewed,in order to provide reference for further research of Calculus Bovis.