ABSTRACT
Objective To compare the clinical value of alpha-fetoprotein (AFP), chemokine (C-C motif) ligand 2 (CCL2) and haptoglobin (HP) in the diagnosis of liver metastasis of colorectal cancer. Methods 256 patients with colorectal cancer in the 983rd Hospital of the Joint Service Support Force of PLAl from Jan. 2016 to Dec. 2018 and 92 healthy subjects (control group) were enrolled. The patients were grouped according to liver metastasis, including liver metastasis group (72 cases) and non-liver metastasis group (184 cases). The serum levels of AFP, CCL2 and HP in different groups were compared. The diagnostic values of AFP, CCL2 and HP in liver metastasis of colorectal cancer were analyzed by ROC curve. Results The serum concentrations of AFP, CCL2 and HP were increased in the control group, non-liver metastasis group and liver metastasis group in order, and the diffences between any two groups were statistically significant (all P<0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The results of diagnostic efficacy for colorectal cancer liver metastases showed that AFP+CCL2+HP had the highest sensitivity (93.05％), HP had the highest specificity (92.75％), CCL2 +HP had the hightest Judden index (0.80), HP had the highest positive predictive rate (75.60％), and AFP + CCL2+HP had the hightest negative predictive rate (97.92％). Conclusions Early diagnosis and prevention of liver metastasis of colorectal cancer can improve the long-term survival rate of patients with colorectal cancer. HP, CCL2 and AFP have important clinical value in early diagnosis of liver metastasis of colorectal cancer.
ABSTRACT
Objective@#To compare the clinical value of alpha-fetoprotein (AFP), chemokine (C-C motif) ligand 2 (CCL2) and haptoglobin (HP) in the diagnosis of liver metastasis of colorectal cancer.@*Methods@#256 patients with colorectal cancer in the 983rd Hospital of the Joint Service Support Force of PLAl from Jan. 2016 to Dec. 2018 and 92 healthy subjects (control group) were enrolled. The patients were grouped according to liver metastasis, including liver metastasis group (72 cases) and non-liver metastasis group (184 cases). The serum levels of AFP, CCL2 and HP in different groups were compared. The diagnostic values of AFP, CCL2 and HP in liver metastasis of colorectal cancer were analyzed by ROC curve.@*Results@#The serum concentrations of AFP, CCL2 and HP were increased in the control group, non-liver metastasis group and liver metastasis group in order, and the diffences between any two groups were statistically significant (all P<0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The results of diagnostic efficacy for colorectal cancer liver metastases showed that AFP+CCL2+HP had the highest sensitivity (93.05%), HP had the highest specificity (92.75%), CCL2+HP had the hightest Judden index (0.80), HP had the highest positive predictive rate (75.60%), and AFP+CCL2+HP had the hightest negative predictive rate (97.92%).@*Conclusions@#Early diagnosis and prevention of liver metastasis of colorectal cancer can improve the long-term survival rate of patients with colorectal cancer. HP, CCL2 and AFP have important clinical value in early diagnosis of liver metastasis of colorectal cancer.
ABSTRACT
Objective To study the role of OIf-1/EBF associated zinc finger protein (OAZ),a transcription factor encoded by a positional systemic lupus erythematosus (SLE) candidate gene,in the function of mesenchymal stem cells (MSC) of SLE patients by silencing this gene.Methods OAZ mRNA levels of bone marrow MSC obtained from 5 SLE patients and 5 healthy controls were detected by real-time PCR.Bone marrow MSC obtained from 6 SLE patients were incubated with specific siRNAs for 3 days,then cells were harvested for OAZ measurement,Idl-3 and CCL2 mRNA levels were tested by real-time PCR,and levels of CCL2 were detected in culture supernatants using ELISA.Differences between groups were analyzed using t-test or MannWhitney test.Results ① OAZ mRNA levels of bone marrow MSC were significantly elevated in SLE patients (0.013±0.016) compared to healthy controls (0.001±0.000,P=0.009).② After OAZ silencing,the expression levels of OAZ,Id1,Id2 and ld3 mRNA were significantly decreased (△Ct 10.3±0.7,15.2±1.6,8.1±1.4,10.5±0.6 vs 8.7±0.7,14.1±1.2,7.1±1.5,9.8±0.6) (P all ＜0.05).③ Both the expression levels of CCL2 mRNA (△Ct 2.2±1.1 vs 3.0±1.1 ) and the levels of CCL2 protein in culture supernatants [(341±29) pg/ml vs (304±19) pg/ml] were significantly increased in OAZ silencing group comparing to those in the control group (P all ＜0.05).Conclusion OAZ gene expression is significantly elevated in bone marrow MSC of SLE patients.OAZ may affect autoantibody production in SLE patients by regulating CCL2 expression.