ABSTRACT
La vacunación con la cepa China es utilizada para prevenir la Peste Porcina Clásica. Pero la presencia de anticuerpos pasivos y la edad de los lechones a la vacunación pueden originar una respuesta humoral activa no satisfactoria. Se vacunaron animales de 7, 21 y 56 días de edad con y sin inmunidad pasiva. Se tomaron muestra de sangre al momento de la vacunación y a los 15 y 45 días posteriores. Los animales respondieron de distintas maneras a la vacunación, según la presencia o no de anticuerpos pasivos. Se observó que a mayor edad de vacunación, mayor era el porcentaje de animales que respondieron a la vacuna en el último muestreo. La técnica de ELISA no permitió detectar anticuerpos a los 15 días de vacunados.
The vaccination with the Chinese strain is used to prevent the Classical Swine Fever. But the presence of passive antibodies and age of piglets at the time of vaccination can originate an unsatisfactory active humoral response. In this study 7, 21 and 56 days animals with and without passive immunity, were vaccinated. Blood samples were taken at the time of vaccination and then at 15 and 45 days later. The animals responded in different ways to vaccination, according to the presence or not of passive antibodies. It was observed that as greater the age of vaccination, greater was the percentage of animals that responded to the vaccine in the last sampling. The ELISA technique did not allow detecting antibodies 15 days after vaccination.
Subject(s)
Animals , Antibody Affinity , Classical Swine Fever , Vaccination/veterinaryABSTRACT
Objective To clone and express the cDNA encoding Schistosoma japonicum tropomyosin. Methods The cDNA was amplified by reverse transcription polymerase chain reaction (RT PCR). The PCR products were ligated with pGEM T vectors and then for transformations. After characterization of white clones by agarose gel electrophoresis, endonucleases digestion and PCR, some recombinant plasmids with inserts were used for sequencing. Then the gene was subcloned into prokaryotic expression vector pQE30 and expression was induced by IPTG. Results The PCR products was 823 bp judged by agarose gel electrophoresis and sequencing. A cDNA encoding S japonicum tropomyosin, except for 14 amino acids at the amino terminus and 2 at the carboxyl terminus, has been constructed and cloned successfully. The colony, designated pGSjcTM12, was sequenced and shown to be 91 1% identical at the nuclei acid level and 98.1% identical in deduced amino acid sequence to that of S mansoni tropomyosin. The gene was subcloned into pQE30 and an expressed protein of about 32 kDa was obtained.Conclusion The cloning and expression of the gene encoding S japonicum tropomyosin had been successfully made.