ABSTRACT
Herein we describe the preparation, characterization and utilization of chitosan nanoparticles for the intracellular delivery of the poorly cell-penetrating antibiotic e.g. Ciprofloxacin, Chlortetracycline hydrochloride and Gentamycin sulfate to improve their treatment of bacterial infections. Chitosan nanoparticles were prepared via the ionic gelation of chitosan with tri polyphosphate anions. Several parameters were studied to optimize the particle size of chitosan nanoparticles, here we select the concentration of chitosan and the concentrations of sodium tri poly phosphate (TPP) as optimizing parameters and the other factors stay constant such as pH of solution and ultrasonication time. Chitosan nanoparticles formed characterized by using FT-IR and transmission electron microscope (TEM). Results show that chitosan nanoparticles and its loaded antibiotics kill and inhibits the growth of gram (+) and gram (-) bacteria tested due to nanoparticles structures, and the antibacterial activity increased with increasing the anti biotic content.
ABSTRACT
OBJECTIVE:To determine the contents of chlortetracycline hydrochloride and tetracaine hydrochloride in compound chlortetracycline hydrochloride ointment.METHODS:Dual wavelength spectrophotometry was used to determine the two components.The absorbance of the two components was determined at wavelength of 311 nm,368 nm and 387.5 nm,respectively;and the contents of which were computed as well.RESULTS:The linear ranges for chlortetracycline hydrochloride and tetracaine hydrochloride were 9.90～29.71 ?g?mL-1 and 5.99～17.98 ?g?mL-1(r=0.999 9),respectively,with average recovery rates at 99.37%(RSD=0.31%,n=9) and 98.31%(RSD=0.23%,n=9),respectively.CONCLUSION:The method is simple,rapid and accurate,and suitable for the determination of compound chlortetracycline hydrochloride ointment.