ABSTRACT
AIM: To establish a method for determining baicalin, jasminoidin, cholalic acid and hyodeoxycholic acid in Qingkailing Granules(chololic acid, hyodeoxycholic acid, baicalin, Fructus Gardeniae, Cornu Bubali, Flos Longicerae Japonicae, Radix lsatidis, Concha Margaritifera). METHODS: Part 1 (determination of baicalin and jasminoidin):The HPLC method was carried out on kromasil~(TM)C_(18) column(4.6 mm×150 mm,5 μm) with acetonitrile-water(10: 90)as mobile phase, gradient elution; the UV detection wavelength was at 238 nm. Part 2 (determination of cholalic acid and hyodeoxycholic acid):The HPLC method was carried out on kromasil~(TM)C_(18) column(4.6 mm×150 mm,5 μm) with acetonitrile-water-phosphonic acid(35:65:0.1) as mobile phase,gradient elution;the UV detection wavelength was at 192 nm. RESULTS: The average recoveries were 99.30% with RSD of 0.2% for baicalin; 99.50% with RSD of 0.4% for jasminoidin; 99.04% with RSD of 0.2% for hyodeoxycholic acid; 99.06% with RSD of 0.4% for cholalic acid; respectively. CONCLUSION: The assay demonstrats that the method is simple,it has the adequate accuracy and selectivity to quantify the four active components in Qingkailing Granules.
ABSTRACT
OBJECTIVE:To study the effects of different extraction methods on the content of cholalic acid from Artificial Bezoar. METHODS: Artificial Bezoar from 3 different habitats was used to extract cholalic acid by hydrolization vs. hydrolization-free method, and the content of cholalic acid was determined by HPLC-ELSD(high performance liquid chromatography with evaporative light scattering detection). RESULTS: The content of cholalic acid in the hydrolyzed sample was higher than that in the non-hydrolyzed sample of the same batch. CONCLUSION: Through hydrolization, the binding cholalic acid in the artificial bezoar was transformed into free cholalic acid so that the content of cholalic acid was increased. Therefore, it is more reasonable to control the quality of Artificial Bezoar by taking the contents of total cholalic acid and free cholalic acid as indexes.
ABSTRACT
OBJECTIVE:To determine the contents of cholalic acid and hyodeoxycholic acid in Qingkailing suppository by HPLC-ELSD(high performance liquid chromatography with evaporative light scattering detection). METHODS: The analytical column was Diamonsil C18(250 mm?4.6 mm, 5 ?m) and the mobile phase consisted of 0.1% formic acid-acetonitrile(gradient elution) with the temperature of the drift tube of the light scattering detector set at 110 ℃ and the flow rate of the nitrogen gas at 2.0 L?min-1. RESULTS: The linear ranges of cholalic acid and hyodeoxycholic acid were 0.59～7.4 ?g(r=0.999 6)and 0.38～4.8 ?g(r=0.999 7),respectively,with their average recoveries at 99.20%(RSD=1.4%,n=6)and 98.57%(RSD=1.8%,n=6), respectively. CONCLUSION: The method is simple, accurate and well-separated, and it is applicable for the quality control of Qingkailing suppository.
ABSTRACT
AIM: To establish a method for determining baicalin,jasminoidin,cholalic acid and hyodeoxycho-lic acid in Qingkailing Granules(chololic acid,hyodeoxycholic acid,baicalin,Fructus Gardeniae,Cornu Bubali, Flos Longicerae Japonicae,Radix Isatidis,Concha Margaritifera). METHODS: Part 1(determination of baicalin and jasminoidin):The HPLC method was carried out on kromasilTMC_18 column(4.6 mm?150 mm,5 ?m) with acetonitrile-water(10∶90)as mobile phase,gradient elution;the UV detection wavelength was at 238 nm.Part 2(determination of cholalic acid and hyodeoxycholic acid):The HPLC method was carried out on kromasilTMC_18 column(4.6 mm?150 mm,5 ?m) with acetonitrile-water-phosphonic acid(35∶65∶0.1) as mobile phase,gradient elution;the UV detection wavelength was at 192 nm. RESULTS: The average recoveries were 99.30% with RSD of 0.2% for baicalin;99.50% with RSD of 0.4% for jasminoidin;99.04% with RSD of 0.2% for hyodeoxycholic acid;99.06% with RSD of 0.4% for cholalic acid;respectively.CONCLUSION: The assay demonstrats that the method is simple,it has the adequate accuracy and selectivity to quantify the four active components in Qingkailing Granules.