ABSTRACT
Objective To investigate the function of mucin 2,mucin 4 on formation of lithogenic bile in patients with calculus of intrahepatic duct.Methods Bile duct mucosa,bile duct wall,bile and plasma were collected from 56 patients with calculus of intrahepatic duct (CID group) and 17 individuals without calculus of intrahepatic duct (control group).The bile duct wall was stained with mucin 2 (MUC2) and mucin 4 (MUC4).Reverse-transcription polymerase chain reaction (RT-PCR) was applied to study the mRNA expressions of MUC2 and MUC4 in the bile duct mucosa.The correlation of the bile duct and serum lipid index was analyzed.Results Serum lipid index in the CID group was significantly higher than control group (all P＜0.05).Biliary total bile acids and bile acids mol percentage were lower in the CID group than control group (both P＜0.05).The expressions of MUC2 was not increased significantly in CID group than the control group (all P＞0.05).The expressions of MUC4 were more significantly increased in CID group than the control group (P＜0.05).The mRNA of MUC4 in the CID group was also more significantly increased than in control group (P＜0.01).There were no correlations between MUC4 expression and the level of biliary total bile acid in the CID group (r=-0.374,P＜0.05).Conclusion The expression of MUC4 in patients with CID was enhanced,which promoted the absorption of bile acid by the mucosal epithelium of the bile duct,and caused a large amount of mucin to be secreted into bile,which may be related to the formation of stony bile.
ABSTRACT
ObjectiveTo explore the feasibility and efficacy of percutaneous transhepatic approach in the treatment of intrahepatic and extrahepatic bile duct stones. MethodsNineteen patients with bile duct stones who were treated in our hospital from January 2000 to October 2014 were enrolled as subjects. In the treatment of bile duct stones, all patients received percutaneous transhepatic approach combined with the application of cholangioscopy and lithotripsy equipment. ResultsIn the 19 patients, 12 had the stones successfully removed in a single session, 5 had the stones completely removed through the liver sinus in three sessions, and 2 had residual stones in intrahepatic tertiary bile ducts. The success rate of lithotomy reached 89.47%(17/19). ConclusionThe percutaneous transhepatic approach achieves minor surgical invasion and slight pain in the treatment of bile duct stones, which can be selectively used based on patients′ condition.
ABSTRACT
ObjectiveTo explore the feasibility and efficacy of percutaneous transhepatic approach in the treatment of intrahepatic and extrahepatic bile duct stones. MethodsNineteen patients with bile duct stones who were treated in our hospital from January 2000 to October 2014 were enrolled as subjects. In the treatment of bile duct stones, all patients received percutaneous transhepatic approach combined with the application of cholangioscopy and lithotripsy equipment. ResultsIn the 19 patients, 12 had the stones successfully removed in a single session, 5 had the stones completely removed through the liver sinus in three sessions, and 2 had residual stones in intrahepatic tertiary bile ducts. The success rate of lithotomy reached 89.47%(17/19). ConclusionThe percutaneous transhepatic approach achieves minor surgical invasion and slight pain in the treatment of bile duct stones, which can be selectively used based on patients′ condition.