1.
Chinese Pharmaceutical Journal
;
(24): 1426-1431, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-859918
ABSTRACT
OBJECTIVE: To model and optimize the reaction conditions of enzymatic esterification of cholesterol with diethenyl decanedioate in isooctane by response surface metodology (RSM).
2.
Chinese Traditional and Herbal Drugs
;
(24): 3289-3295, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-855010
ABSTRACT
Objective: To synthesize cholesteryl vinyl hemi-sebacate from cholesterol and divinyl sebacate in non aqueous phase. Methods: TLC, MS, and NMR were used to identify the structure of production; the technological conditions of enzymatic esterification were determined through orthogonal test. Results: The best condition: isooctane 5 mL, reaction temperature 35°C, reaction time 24 h, Candida rugosa Lipase 10 mg/mL, molar ratio of cholesterol to divinyl sebacate 1:6. Conclusion: The highest esterification rate is above 95%.