ABSTRACT
Objective To explore the effect and injury mechanism of reactive oxygen species (ROS) after spinal cord injury (SCI) through detecting the dynamic changes of malonyldialdehyed (MDA)content in spinal cord and observing neurocyte apoptosis and correlation apoptosis factor expression after SCI. Methods Totally 132 adult SD male rats were randomly divided into three groups: sham group, SCI group, methylprednisolone (MPSS) group. The SCI of SD rats was performed by Allen's weight dropping way to impact on the posteriors of spinal cord T_(10). The contents of MDA were determined by chromatometry, the expression of Caspase-3 and Bcl-2 family in the injured spinal cord was detected by immunohistochemical staining;Apoptotic cells were detected by using fluorometric terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (fluorometric TUNEL) staining. Results The content of MDA in the injured cord increased significantly after SCI;R3eached the peak at 6 hours and 3 days post-injury, then dropped down gradually, then was back to the normal level after 7 days. The number of TUNEL labeling positive cells of SCI group increased at 6 hours post-injury;R3eached the peak at 3 days, then dropped down gradually;Bcl-2, Bax protein began to increase at 6 hours post-injury;R3eached the peak at 5 days after injury, then dropped down gradually. Caspase-3 protein began to increase at 6 hours post-injury;R3eached the peak at 3 days after injury, then dropped down gradually. The content of MDA, the number of TUNEL labeling positive cells, the expression of Caspase-3 and Bax of MPSS group decreased significantly than that of SCI group at the same time;R3espectively, while Bcl-2 protein was up-regulated after administration of MPSS.Conclusion ROS could promote the expression of Caspase-3 and degrade the ratio of Bcl-2/Bax to induce apoptosis of neurocyte, which might play significantly role in the process of secondary SCI. In addition, MPSS exerts neuroprotective effects against ROS toxicity, which might be of importance and might contribute to their clinical efficacy for the treatment of SCI.
ABSTRACT
ObjectiveTo examine the protective effects of 17-β estradiol on the experimental model of spinal cord injury (SCI) rats. Methods One hundred and eighty male Sprague Dawley (SD) rats, after Allen' s model, SD rats were divided into three groups: the sham group, the acute spinal cord injury (control groups) and the acute spinal cord injury supplying with 17-β estradiol treatment group. SCI was made by Allen's weight dropping, impacting on the posteriors of spinal cord T10. The content of malonyldialdehyed (MDA), glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined by chromatometry. The expressions of Caspase-3 and Bcl-2 family in the injured spinal cord were detected by immunohistochemical staining. Results The BBB scores at each time point in 17-β estradiol treatment group were significantly higher than that in SCI group (P<0.05). The contents of GSH, SOD, GSH-Px and the expression of Bcl-2 protein at the majority of time point in 17-β estradiol treatment group were significantly higher than that in SCI group(P<0.05), however, the MDA, Caspase-3 and Bax were markedly decreased (P<0.05). Conclusions This study suggests that 17-β estradiol administration might prevent the cells from SCI-induced apoptosis by triggering to reduce the oxidative stress.
ABSTRACT
Objective To establish the determination method of total polyphenols in vitis amurensis seed extract.Methods Using gallic acid as reference substance,the content of total polyphenols in Vitis amurensis seed extract was determined by the optimum conditions of Folin-Ciocalteu chromatometry.Results There was a good linearity between gallic acid concentration and absorbance in the range 0.002-0.010 g?L-1(Y=86.243X+0.007,r=0.9996);the precision RSD was 0.14%;the stability RSD was 1.77%;the repeatability RSD was 1.44%;the average recovery rate was 102.00%(RSD=1.37%).Conclusion This method is simple,fast and has high sensitivity and good reproducibility.
ABSTRACT
Objective To investigate the effect of age on activity of cholineacetyltransferase(ChAT) and acetylcholinesterase(AChE) in cerebral cholinergic nerve system of rat.Method According to age,male Sprague-Dawley rats were divided into the following 3 groups at random: 4～5 weeks old group,4～5 months old group,14～15 months old group(n=10,respectively).Chromatometry was used to assay the activity of ChAT and AChE in cerebral cortex,hippocampus and corpus striatum.Results In 14～15 months old rats,activity of ChAT and AChE in cerebral cortex,hippocampus and corpus striatum was prominantly reduced,as compared with that in 4～5 months old rats and in 4～5 weeks old rats(ChAT: P0.05).Conclusion There is a negative correlation between age and activity of ChAT and AChE in cerebral cholinergic nerve system.
ABSTRACT
OBJECTIVE: To determine the contents of flavonoids in different part and different harvesting seasons of Hypericum japonicum.METHODS: The total content of flavonoids in H.japonicum was determined by colorimetry and the contents of quercetin,rutin and isorhamnetin in H.japonicum were determined by HPLC.RESULTS: The H.japonicum harvested in different seasons showed no significant difference in terms of the contents of total flavonoids.H.japonicum from Guangxi province contained no isorhamnetin but which was rich in the contents of quercetin and rutin.In H.japonicum,the content of total flavonoids was rich in the seeds and leaves but poor in the roots,and hardly had isorhamnetin,quercetin and rutin been detected in the roots.CONCLUSION:H.japonicum harvested in the spring season is low and small in size with low-yield;therefore,fall is its optimal harvesting season.Furthermore,the specification in Chinese Pharmacopeia that the medicinal part of H.japonicum is the entire herb has been proved to be scientific.