ABSTRACT
OBJECTIVE: In previous studies, it was found that sulfur fumigation had a destructive effect on chemical constituents of Chrysanthemum Flos. To explore the intestinal absorption of Chrysanthemum Flos after sulfur fumigated. METHODS: The everted rat intestinal sacs were used, and the absorbed was analyzed constituents by UPLC-QTof-MS and HPLC. RESULTS: For sulfur fumigated Chrysanthemum Flos, the contents of flavonoid glycosides were declined in intestinal absorption, while the contents of flavonoid aglycones were not obviously increased. CONCLUSION: Sulphur fumigation lead to the changes of Chrysanthemum Flos, which affects its intestinal absorption.
ABSTRACT
Objective: To investigate the oxidation resistance and removing chloasma function of the extract from Hangzhou Chrysanthemum Flos, Angelicae Sinensis Radix, and Salviae Mmiltiorrhizae Radix et Rhizoma in cholasma model induced by progesterone and ultraviolet radiation. Methods: The cholasma model was establised with progesterone (20 mg/kg) by im injection or ultraviolet radiation in female mice. The effects of each group on GSH-Px, SOD, tyrosinase (TYR), MDA, NO, T-AOC, and lipofuscin in skin and liver were detected, and hemorheology and dermatopathology were observed. Results: The result showed that the groups with the extract from Hangzhou Chrysanthemum Flos, Angelicae Sinensis Radix, and Salviae Mmiltiorrhizae Radix et Rhizoma could signifcantly increase the activity of GSH-Px and SOD and decrease the contents of MDA and TYR in skin and liver. Such effect conduced that a decrease of melanin production in the nude mice model was observed. Conclusion: All the extracts from Hangzhou Chrysanthemum Flos, Angelicae Sinensis Radix, and Salviae Mmiltiorrhizae Radix et Rhizoma have the effect of removing chloasma to various degrees, and the mechanism may be related to enhancing oxidation resistance, deccreasing TYR activity, inhibiting melanocyte proliferation, and improving hemorheology.