MUC8 as a Ciliated Cell Marker in Human Nasal Epithelium / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: To examine the MUC8 mRNA expression patterns according to the mucociliary differentiation of the normal human nasal epithelial (NHNE) cells, and to investigate the localization of the MUC8 proteins in the nasal polyps. MATERIALS AND METHODS: The passage-2 NHNE cells were cultured using an air-liquid interface technique and nasal polyp specimens. On the 2, 7, 14, and 28 days after confluence, the ciliated cells were counted using cytospin slide immunostaining using H6C5 and beta-tubulin, and the MUC8 mRNA levels were determined using real-time quantitative PCR. After synthesizing the polyclonal anti-MUC8 peptide antibodies, MUC8 immunostaining was preformed using the nasal polyps. The MUC8 mRNA and protein levels were determined with the NHNE cells treated with IL-1beta (10 ng/ml for 24 hours) using RT-PCR and Western blot analysis. RESULTS: The increasing pattern of the number of ciliated cells as well as the MUC8 gene expression level with increasing culture time in the NHNE cells was quite similar. MUC8 was expressed in the ciliated cells of the human nasal polyps. The MUC8 protein level as well as the mRNA level was up-regulated as a result of the IL-1beta treatment. CONCLUSIONS: This study indicates that the MUC8 protein is expressed in ciliated cells from the human nasal epithelial cells and is up-regulated by the IL-1beta treatment. These results suggest that the MUC8 gene and protein expression levels might be used as a ciliated cell marker in the human nasal epithelium.

An Electron Microscopic Study on Cytoplasmic Organelles of the Cilicated Cell in Tracheal Epithelium in o-Chlorobenzylidene malononitrile (CS)gas exposed rat / 체질인류학회지

The effects of tear gas, o -chlorobenzylidene malononitrile (CS) on the cytoplasmic organelles were studied in the ciliated cell of rat tracheal epithelium. Albino rats (Sprague -Dawley strain), weighing about 150gm, were used as experimental animals. The experimental animals were exposed to 2.0 g/m 3 of CS gas for 20 minutes per day for the succesive 3 days. The experimental animals were sacrified at 1, 3, 6, 12 hours and 1, 3 and 5 days after final exposure to CS gas. Specimens obtained from the trachea were pre -fixed in 2% glutaraldehyde -2.5% paraformaldehyde and post -fixed in the 1% osmium tetroxide for electron microscopic study. The results obtained were as follows: 1. In 1 hour CS gas exposed group, rough endoplasmic reticulum with dilated cisternae, and mitochondria with disrupted double membrane in the ciliated cells are found. 2. In 3 hours and 6 hours CS gas exposed groups, dilated, segmented and sacculated cisterane of rough endoplasmic reticulum, mitochondria with dissolved cristae and disrupted double membrane, and Golgi complex with atrophied cisternae are observed in the ciliated cell. 3. In 12 hours CS gas exposed group, some mitochondria with swollen cristae is found in the ciliated cell. 4. In 1 day CS gas exposed group, mitochondria with dissolved cristae, Golgi complex with hypertrophied cisternae, and autophagic vacuole are found. 5. In 3 day and 5 day CS gas exposed groups, numerous mitochondria, well -developed rough endoplasmic reticulum, and supranuclear Golgi complex are found in ciliated cell. The results of the present study suggest that the o -chlorobenzylidene malononitrile (CS) gas is cytotoxic to the ciliated cells in tracheal epithelium inducing some degenerative changes, which are recovered with the lapse of time.

CYCLIC VARIATIONS OF SURFACE STRUCTURE IN THE HUMAN OVIDUCTAL EPITHELIUM DURING THE MENSTRUAL CYCLE / 解剖学报

Oviducts were removed from 12 reproductive women at specific times duringthe menstrual cycle.The surface structure of oviductal epithelium have mainlyexamined by scanning electron microscopy.At proliferative stage,the growing ciliaand microvilli were indeed detected.The typical pattern at midcycle was that thecilia and microvilli had well developed and reached their full length.At secretorystage,however,ciliation was much less extensive than that at midcycle.The surfacephenomenon of some cilia had lost from ciliated cells but some still retained.Nonciliated cells had more evident changes than ciliated cells.At early secretory stage,the cobblestone-like nonciliated cells bulged into the lumen.Then,the centerof these cells exhibited concave with some cytoplasmic buds which were probably asrepresentation of their apocrine secretory activity.At late secretory stage,manynonciliated cells showed cobblestone-like bulged into the lumen again.The epithe-lium also showed a tiny minority small cells with abundant and well developedmicrovilli like those at proliferative stage.The cyclic changes in the oviductalepithelium were very pronounced in the fimbriae and infundibulum,less distinct inthe ampulla and very slight in the isthmus.