ABSTRACT
Objective: To investigate the secondary metabolites from Chinese marine Sponge Cinachyrella australiensis. Methods: Column chromatography techniques including HPLC were used for the separation and purification of the compounds, and extensive spectral analyses including various 2D NMR spectra were employed for structure elucidation. Results: Nineteen compounds were obtained ,including 2 methyoxy 6,12,15 trien 8 yne octadecanoic acid(1), 2 benzenedicarboxylic acid dibutyl ester(2), 1,2 benzenedicarboxylic acid bis(2 ethylhexyl)ester(3), ( ) (3S) 1,2,3,4 tetrahydro ? carboline 3 carboxylic acid(4), L Tryptophan (5), p hydroxylbenzaldehyde (6), p hydroxyl benzylethanol(7), p hydroxyl benzyl propanol(8), cholesta 4 en 3 ol(9), 2 methyl 6 amino 9 (2 deoxy ? D ribofuranosyl purine(10), 2' Deoxyadenosine (11), 6 amino 9 ? D ribofuranosyl 9H purine (12),uracil(13), thymine(14), thymidine(15), 1 (2 deoxy ? D Ribofuranosyl)uracil(16), 1 ethyl ? (2 deoxy) ? D ribofuranos(17),isolumichrome(18),and zarzissine(19).Conclusion:Compounds 1 and 18 are new natural products,and compounds 2 to 17 as well as 19 are isolated from this species for the first time.
ABSTRACT
Object To search for bioactive secondary metabolites from marine organisms, the marine sponge Cinachyrella australiensis was collected from Hainan Island, Southern China Sea to develop the efficient drug for human beings. Methods Silic gel flash chromatography in association with reverse phase semipreparative HPLC was performed for the isolation and purification of the ethanol extracts, and extensive spectroscopy including 2D NMR spectra as well as the comparison of the spectral data with those reported in literatures was applied for the structure elucidation. Results Seven nucleotidoids were isolated and identified. They are named as 2-methyl-6-amino-9-(2-deoxy-?-D-ribofuranosyl)-purine (Ⅰ), 2′-deoxyadenosne (Ⅱ), 6-amino-9-?-D-ribofuranosyl-9H-punne (Ⅲ), uracill (Ⅳ), thymine (Ⅴ), thymidine (Ⅵ), 1-(2-deoxy-?-D-ribofuranosyl) uracil (Ⅶ). Conclusion The sponge C. australiensis is systemically separated, and then two kinds of nucleotidoids compound are extracted.