ABSTRACT
Objectives: The present study was designed to examine the efficacy of Cissus quadrangularis paste on fracture healing in artificially induced fractured rabbits. Methods: Fifteen rabbits were separated into three groups namely A, B, and C. Veldt grape paste was applied in groups B and C (Treatment Group) by close reduction and open reduction methods of fracture management; respectively, while group A was kept as control. The blood parameter and fracture healing properties in all animals have been monitored and examined routinely during the study period. Results: Both treated groups revealed lower serum calcium levels (SCL) than the control group after 24 hours of fracture that became within the normal range on the 14th day. Fracture healing in the treated groups has been commenced more rapidly than the control group with complete bridging of discontinuity by a distinct osseous callus in the fracture line on day 7 and complete effacing of fracture line on day 14. Conclusion: We did not find any type of anomalousness, clinical deviations, and alteration of serum calcium level on the 14th day of the fracture in treated animals hence Veldt Grape paste could be readily applicable to the management of the fracture in animals.
Objetivos: avaliar a eficácia da pasta de Cissus quadrangularis na consolidação de fraturas em coelhos fraturados artificialmente. Metodos: quinze coelhos foram separados em três grupos (A, B e C). A pasta foi aplicada nos grupos B e C (Grupo de Tratamento) pelos métodos de redução fechada e redução aberta de gerenciamento de fraturas; respectivamente. O grupo A foi mantido como controle. O parâmetro sanguíneo e as propriedades de cicatrização de fraturas em todos os animais foram monitorados e examinados, rotineiramente, durante o período do estudo. Resultados: ambos os grupos tratados revelaram níveis séricos de cálcio (SCL) mais baixos do que o grupo controle, após 24 horas de fratura que se tornou normal no 14º dia. A cicatrização da fratura nos grupos tratados foi iniciada mais rapidamente do que o grupo controle, com ponte completa de descontinuidade por um calo ósseo distinto na linha de fratura no dia 7 e apagamento completo da linha de fratura no dia 14. Conclusao: não encontramos nenhum tipo de anomalia, desvios clínicos e alteração do nível sérico de cálcio no 14º dia da fratura nos animais tratados, portanto, a pasta Veldt Grape pode ser aplicável ao manejo da fratura em animais.
Subject(s)
Fracture Healing , Cissus , Ointments , Therapeutics , Bony Callus , Fractures, Bone , Goals , Animals, Laboratory , MethodsABSTRACT
Cissus quadrangularis L.belongs to the family vitaceae and is an indigenous medicinal plant of India. The present study was aimed to investigate the effect of Cissus quadrangularis on histological changes of normal and cissus quadrangularis plant extract treated fresh water fish, Oreochromis mossambicus tissues samples (gill, liver and muscle ) of Oreochromis mossambicus, at 7 , 14 and 21days.The fish exposed with Cissus quadrangularis when compared with control fish. The present study concludes that Cissus quadrangularis is an beneficial to the growth and development of fishes
ABSTRACT
Herbal medicine has been regarded as a safer and more natural method to promote health and alleviate illness and has gained notable popularity. Plants continue to be the primary source of new chemicals and drugs and hence play a pivotal role in the continual improvement in therapeutic medicine. India has a huge asset of indigenous plants and minerals which have been broadly used for therapeutic claim. One such plant which has immense benefits derived from it is Cissus quadrangularis [CQ] colloquially known as hadjod or pirandai. CQ is one of the most significant plants. However, practically the entirety of its parts are utilized in medicine among which seeds, stems, roots, and shoots are the most significant parts. Concentrates and powders of Cissus quadrangularis have been utilized for a long time to aid bone and tissues healing, as an antioxidant, for relieving pain & swelling, to fight against infection, as an anabolic, and also to help in weight reduction. The most important usage has been for bone regeneration and fracture healing. Numerous studies have been done to prove its pharmacological effects and to substantiate its use clinically. The aim of this review is to elaborate on CQ, its ethnopharmacology, phytochemistry and principle constituents, pharmacological actions, formulations and to review previous literature such as animal studies, in vitro studies and clinical trials to understand in detail about the applications of this plant in medicine and dentistry
ABSTRACT
The healing of a fracture is one of the most remarkable of all the repair processes in the body since results not in a scar but in the actual reconstitution of the injured tissue in something very like its original form. It is not to be expected therefore that the mechanisms controlling such a process will be easily elucidated and indeed they involve problems of cellular homeostasis which are among the most fundamental in biology. If it is not quite thou cunning'st pattern of excelling nature, then it is something quite close to it and a great deal of that pattern. Present study shows experimental evaluation of effect of Asthishrunkhala (Cissus quadrangularis) on bone healing in fractured bone by histo-pathological study. During study concerned literature was reviewed critically. The study was divided into phytochemical, pharmacological and histopathological study. Drug was identified and authenticated from authorized institute and phytochemical screening done after successive extraction of extract. Two groups were made. First Group A (Treatment group) was further divided into six subgroups containing five animals each received extract of Cissus quadrangularis 500mg/kg body weight, Group B is control group. After completing the study it was observed that mature bone or bone repair process seen weakly at 2nd – 3rd week proving the acceleration of bone healing process with gradual re-modeling. Statistical analysis shows that histopathological study in group A found to be highly significant p<0.001 from 4th week onwards. Overall the bone healing process shows 10% faster healing period in group A than group B.
ABSTRACT
The present investigation involves antimicrobial and phytochemical evaluation of Cissus quadrangularis L. The antibacterial activity of Cissus quadrangularis was performed using disk diffusion method. The Results of study proved prompt efficacy of herbal extract against S. aureus and E. coli. The concentration dependent antibacterial activity of extract was observed against both organisms. Study also involves phytochemical investigation of herbal extract using HPTLC, IR and UV-Visible spectrophotometer. The result of study indicated that the methanolic extract possessed most potent antibacterial activity as compared to other extract. The antibacterial activity increases with the concentration and results indicated that the diameters of zone inhibition of the extract were comparable with the standard drug. The antimicrobial potential of plant extract may be attributed to the presence of specific phytoconstituents.
ABSTRACT
Background: Infants of the diabetic mother are known to have reduced bone mineral content and hypocalcemia. Earlier, it has been shown that petroleum ether extract of Cissus quadrangularis (PECQ) can enhance the fetal skeletal ossification in normal rats. The present study was designed to evaluate the effect of PECQ on skeletal growth in the neonatal rats of streptozotocin-induced diabetic rats. Methods: After confirmation of diabetes, the diabetic and non-diabetic female Wistar rats were kept for mating with healthy male rats. After positive vaginal smear test, the pregnant rats were divided into three groups; the normal (non-diabetic) control (NC), diabetic control (DC), and diabetic+CQ (D+CQ) groups. The rats in the D+CQ group were given PECQ (500 mg/kg B.Wt), whereas animals in NC and DC groups were given 0.5% carboxy methyl cellulose, throughout the gestational period. Femur from the 1 week old neonatal rats from each group was collected randomly and subjected to histological analysis. Results: Thickness of trabecular bone and periosteum was significantly reduced in the neonates of DC rats compared with the neonates of NC group. Pretreatment with PECQ significantly improved the thickness of trabecular bone and periosteum compared with neonatal rats of DC group. No significant differences were observed in the medullary cavity width of femur between the groups. Conclusion: Data from the present study suggest that the PECQ can effectively attenuate the diabetes-induced reduction in the early skeletal growth. However, further research is warranted to evaluate the exact mechanism of action of phytochemical constituents of PECQ that can cross the placental barrier.
ABSTRACT
Background: Delayed fetal skeletal ossification is one of the known complications of maternal diabetes. Objective: The present study was designed to evaluate the protective role of petroleum ether extract of Cissus quadrangularis (PECQ) on diabetes‑induced delayed fetal skeletal ossification. Materials and Methods: Female Wistar rats were rendered diabetic with streptozotocin (STZ, 40 mg/kg, intraperitonial) before mating. After confirmation of pregnancy, the pregnant rats were divided into three groups: normal control group, diabetic control group, and diabetic + CQ group. The diabetic + CQ group pregnant rats were treated with PECQ (500 mg/kg body weight) throughout their gestation period. Immediately after delivery, pups were collected from all three groups and processed for alizarin red S–alcian blue staining in order to examine the pattern of skeletal ossification. Results: Fewer ossification centers and decreased extent of ossification of forelimb and hindlimb bones were observed in the neonatal pups of diabetic control group as compared to those in the normal control group. PECQ pretreatment significantly restored the ossification centers and improved the extent of ossification of forelimb and hindlimb bones in the neonatal pups of diabetic + CQ group as compared to those in the diabetic control group. Conclusions: The results suggested that PECQ treatment is effective against diabetes‑induced delayed fetal skeletal ossification. However, further studies on the isolation and characterization of active constituents of PECQ, which can cross the placental barrier and are responsible for the bone anabolic activity are warranted.
ABSTRACT
OBJECTIVE: To evaluate the effects of the petroleum ether extract of Cissus quadrangularis on the proliferation rate of bone marrow mesenchymal stem cells, the differentiation of marrow mesenchymal stem cells into osteoblasts (osteoblastogenesis) and extracellular matrix calcification. This study also aimed to determine the additive effect of osteogenic media and Cissus quadrangularis on proliferation, differentiation and calcification. METHODS: MSCs were cultured in media with or without Cissus quadrangularis for 4 weeks and were then stained for alkaline phosphatase. Extracellular matrix calcification was confirmed by Von Kossa staining. marrow mesenchymal stem cells cultures in control media and osteogenic media supplemented with Cissus quadrangularis extract (100, 200, 300 µg/mL) were also subjected to a cell proliferation assay (MTT). RESULTS: Treatment with 100, 200 or 300 µg/mL petroleum ether extract of Cissus quadrangularis enhanced the differentiation of marrow mesenchymal stem cells into ALP-positive osteoblasts and increased extracellular matrix calcification. Treatment with 300 µg/mL petroleum ether extract of Cissus quadrangularis also enhanced the proliferation rate of the marrow mesenchymal stem cells. Cells grown in osteogenic media containing Cissus quadrangularis exhibited higher proliferation, differentiation and calcification rates than did control cells. CONCLUSION: The results suggest that Cissus quadrangularis stimulates osteoblastogenesis and can be used as preventive/alternative natural medicine for bone diseases such as osteoporosis.