ABSTRACT
Objective To evaluate the accuracy of using prothrombin time (PT ) derivative algorithm(PT‐Fib) method to detect fibrinogen(Fib)content in plasma samples with Sysmex CA7000 automatic coagulometer and its correlation with results of Clauss method .Methods Clauss method and PT‐Fib method were used to detect Fib concentration in plasma of patients′ blood samples by Sysmex CA7000 automated blood coagulation analyzer and supporting reagent .Data were randomly collected as the Fib content were in 1 .00 - 1 .99 ,2 .00 - 2 .99 ,3 .00 - 3 .99 ,4 .00 - 4 .99 g/L detected by PT‐Fib method ,each range with 21 patients .The difference and correlation of two methods were analyzed .Results There was significant statistical difference between PT‐Fib meth‐od and Clauss method(P< 0 .05) when the level of Fib detected by PT‐Fib method were 1 .00 - 1 .99 ,2 .00 - 2 .99 ,3 .00 - 3 .99 , 4 .00 - 4 .99 g/L (n= 21) .The result of the PT‐Fib method was obviously lower than those of the Clauss method on average 15 .2%( - 4 .9% - 38 .3% ) ,27 .2% ( - 0 .9% - 49 .4% ) ,24 .6% ( - 3 .7% - 35 .6% )and 32 .7% (15 .7% - 48 .2% )respectively ,and results of the two methods were positively correlated .Conclusion Fib value calculated by the PT‐Fib method in the fully automatic coagu‐lation Sysmex CA7000 analyzer is obviously lower than that of the Clauss method .It is suggested that the Fib detection should be changed to adopt Clauss method while detecting Fib content in plasma samples with Sysmex CA7000 automatic coagulometer .
ABSTRACT
Objective To comparatively analyze the results of the PT derivative method and the Clauss method for detecting fi-brinogen(Fib) by the automated coagulation analyzer and to explore their accuracy and clinical value .Methods Fib was detected in 156 blood samples selected from the patients in this hospital from March to May 2013 ,25 normal control samples and 13 positive samples of D-dimer (DD) by the TP derivative method and the Clauss method .Results The Clauss method and the PT derivative method by Sysmex CA-1500 coagulation analyzer had good repetitive rate in measuring Fib for 25 times ;when Fib0 .05);when Fib in the range of 4 .1 -5 .0 g/L ,the detection results had statistically significant difference (P5 .0 g/L ,the detection results had statistically significant difference (P<0 .05);in 13 DD positive samples ,the detection results of the PT derivative method were significantly higher than those of the Clauss method with statistically significant difference (t=8 .530 , P<0 .05) .Conclusion The difference exists between the PT derivative method and the Clauss method for detecting Fib ,but the ac-curacy of the Clauss method is higher than its specificity ,the Clauss method should be the first choice for the clinical determination of plasma Fib .