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Aim: This study was designed to evaluate the therapeutic potential of adipose tissue-derived mesenchymal stem cells (ATMSCs) compared to bone marrow-derived mesenchymal stem cells (BM-MSCs) on the regeneration of surgically createdcleft alveolus in dogs. Methods: Split mouth experimental study was performed on twelve healthy mongrel dogs. The dogswere divided into two groups (A and B): Ingroup A, the surgically created alveolus was transplanted with AT-MSCs,scaffold and growth factors at the experimental side (right side of the maxilla). Ingroup B, the surgically created alveoluswas transplanted with BM-MSCs, scaffold and growth factors at the experimental side (right side of the maxilla). In thecontrol side (left side of the maxilla), the surgically created alveolus was transplanted with scaffold and growth factors only.The flaps were replaced and sutured with resorbable sutures. Bone regeneration was evaluated clinically andradiographically after 1.5 and 3 months following dogs’ scarification. The data were evaluated with descriptive and t-testmethods (p=0.05). Results: Stem cells whether AT-MSCs or BM-MSCs accelerate the healing and regeneration of thedefected area by increasing the bone width and surface area; providing the bone quantity and quality as early as 1.5 and 3months. Conclusions: AT-MSCs and BM-MSCs are attractive tools in bone regeneration. AT-MSCs in experimental studiesshowed that their effectiveness is comparable to BM-MSCs, in addition to its low cost, ease of harvesting and saferprocedure to obtain stem cells as well as less risk of infection.
ABSTRACT
Objective To pursue a more efficient and effective treatment for cleft lip and palate deformities. Methods Patients with unilateral complete cleft lip and palate at their age of 9 years after were chosen for simultaneous cleft alveolar repair and nasal deformity correction. Muco-periosteal pocket and iliacgranular bone was prepared, and bone grafting was performed conventionally. At the same time of iliac cancellous bone harvesting, a cortical plate was taken and sculpted into a strut of 18 mm in length, 6 mm in width, and 1.5 mm in thickness. A flying bird incision was made at the alar ram and across columella in a V-shape. Then the alar cartilage was detached from the overlying skin, a socket was made at the site of anterior nasal spine. The strut was inserted into the socket between the two medial crura of the alar cartilage. The medial crura was lift 3 mm above the superior edge of the strut, and mattress suture technique was used to secure the bilateral medial crura to the strut graft. Results 24 patients were treated by this technique. All the patients healed uneventfully. Depressed alar base, tilted columella and lower nasal tip were corrected satisfactorily. Conclusion There is no interference in simultaneous cleft alveolar bone grafting and rhinoplasty. Septum strut can provide favorable support for tilted nasal structure and satisfactorily correct nasal deformities. Simultaneous with alveolar grafting, it is much easier in harvesting, and the time of anesthesia and operation is also decreased.[
ABSTRACT
Samsung Medical Center, Sungkyunkwan university, School of Medicine The cleft alveolus is one of three parts in cleft deformity. The purpose of cleft alveolus bone grafting is the recovery of normal esthetics, occlusion and speech. If a bony defect is extended to the nasal floor, especially wide bony defect at the ala base, it is difficult to condense the cancellous bone during bone transplantation and to reconstruct the normal anatomy at the alar base. We treated with above mentioned cleft alveolus patients using the autogenous cortical bone effectively. We report this technique with two cases and the literatures review.
Subject(s)
Humans , Bone Transplantation , Congenital Abnormalities , EstheticsABSTRACT