ABSTRACT
Objective: To provide molecular evidence for identification of medicinal plants in Clematis L., sequencing and comparison of ITS from C. uncinata, C. henryi, C. chinensis, C. finetiana, C. lasiandra, C. patens, C. apiifolia, and C. patens ssp. tientaiensis were performed. Methods: ITS sequences were amplified from leaf genomic DNA by PCR. Sequence features were compared and analyzed using Clustal X, MEGA, and DNASTAR softwares. Results: Between ITS1 and ITS2 of the eight medicinal plants in Clematis L., the length of ITS region varied from 534-561 bp with 50 variable sites and 22 parsimony information sites. The smallest genetic distance was observed between C. patens ssp. tientaiensis and C. patens, and the largest existed between C. patens ssp. tientaiensis and C. apiifolia revealing their farest genetic relationship. Sequences were submitted to NCBI database with the registry numbers of JF714638-JF714645. Conclusion: ITS sequences of the eight medicinal plants in Clematis L. are obtained, which could provide a foundation for molecular identification.
ABSTRACT
Objective To study the chemical constituents of Clematis manshurica. MethodsThe extract from the roots and rhizomes of C. manshurica was subjected to chromatography on silica gel and Sephadex LH-20 column. The compounds obtained were identified on the basis of their physicochemical and spectroscopic evidences. ResultsEleven compounds were isolated and their structures were elucidated as squalene (Ⅰ), friedelin (Ⅱ), hexacosoic acid (Ⅲ), ?-sitosterol (Ⅳ), stigmasterol (Ⅴ), oleanolic acid (Ⅵ), ?-daucosterol (Ⅶ), 5-hydroxymethyl-2-furaldehyde (Ⅷ), methyl 3, 4-dihydroxy-phenyl lactate (Ⅸ), 5R-dihydro-5-hydroxymethyl-2(3H)-furanone (Ⅹ), 5R-5-hydroxymethyl-2(5H)-furanone (Ⅺ). ConclusionAll the compounds except for ?-sitosterol are isolated from the plant for the first time.